################################## # NO CUT ENZYMES ( 157 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AcyI AfaI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BanII BbeI BbrPI BbvCI BcgI BciVI BclI BfrI BglII BlnI BpmI BsaAI BsaHI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I Cfr10I ClaI CpoI DraI DraII DraIII DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoO109I EcoO65I EcoRI EcoRV FauI FbaI FseI FspI HaeII HgaI Hin1I HindIII HpaI KasI KpnI Ksp632I KspI MfeI MluI MroI MunI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshBI PstI PvuI RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SspI SwaI TaqI Tth111I TthHB8I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AclI 268 AACGTT AA^CGTT AcsI 918 AAATTT [GA]^AATT[CT] ApoI 918 AAATTT [GA]^AATT[CT] BamHI 633 GGATCC G^GATCC BanI 852 GGCACC G^G[CT][GA]CC BbsI 339 GTCTTC GAAGAC(2/6) BcnI 858 CCCGG CC^[GC]GG BfaI 742 CTAG C^TAG BglI 691 GCCACCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 700 GCTGAGC GC^T[ACGT]AGC Bpu1102I 700 GCTGAGC GC^T[ACGT]AGC BpuAI 339 GTCTTC GAAGAC(2/6) BsaBI 84 GATCTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 220 GGTCTC GGTCTC(1/5) BsmI 942 GCATTC GAATGC(1/-1) BspMI 845 ACCTGC ACCTGC(4/8) CelII 700 GCTGAGC GC^T[ACGT]AGC CfoI 177 GCGC GCG^C DsaI 767 CCATGG C^C[GA][CT]GG EcoNI 543 CCTCCACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT14I 767 CCATGG C^C[AT][AT]GG EcoT22I 940 ATGCAT ATGCA^T HapII 859 CCGG C^CGG HhaI 177 GCGC GCG^C HinP1I 177 GCGC G^CGC HincII 364 GTTGAC GT[CT]^[GA]AC HindII 364 GTTGAC GT[CT]^[GA]AC HpaII 859 CCGG C^CGG MaeI 742 CTAG C^TAG MaeII 269 ACGT A^CGT MaeIII 10 GTGAC ^GT[ACGT]AC MamI 84 GATCTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MseI 916 TTAA T^TAA MspA1I 591 CAGCTG C[AC]G^C[GT]G MspI 859 CCGG C^CGG NciI 858 CCCGG CC^[GC]GG NcoI 767 CCATGG C^CATGG NsiI 940 ATGCAT ATGCA^T PshAI 214 GACCTGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC Psp1406I 268 AACGTT AA^CGTT PvuII 591 CAGCTG CAG^CTG SfaNI 32 GCATC GCATC(5/9) SmlI 493 CTCAAG C^T[CT][GA]AG SphI 804 GCATGC GCATG^C StyI 767 CCATGG C^C[AT][AT]GG Tru9I 916 TTAA T^TAA Tsp45I 10 GTGAC ^GT[GC]AC Tsp509I 919 AATT ^AATT XcmI 113 CCATCTCCATCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AciI 561 CCGC CCGC(-3/-1) 469 GCGG BalI 377 TGGCCA TGG^CCA 833 TGGCCA BbvI 252 GCAGC GCAGC(8/12) 455 GCTGC BmyI 684 GGGCAC G[AGT]GC[ACT]^C 713 GTGCCC BsmAI 104 GTCTC GTCTC(1/5) 221 GTCTC Bsp1286I 684 GGGCAC G[AGT]GC[ACT]^C 713 GTGCCC BsrI 436 ACTGG ACTGG(1/-1) 816 ACTGG EaeI 377 TGGCCA [CT]^GGCC[GA] 833 TGGCCA Fnu4HI 252 GCAGC GC^[ACGT]GC 455 GCTGC ItaI 252 GCAGC GC^[ACGT]GC 455 GCTGC MluNI 377 TGGCCA TGG^CCA 833 TGGCCA MscI 377 TGGCCA TGG^CCA 833 TGGCCA MslI 243 CACAGTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 762 CATTCCCATG NlaIV 633 GGATCC GG[ACGT]^[ACGT]CC 852 GGCACC NspI 179 GCATGT [GA]CATG^[CT] 804 GCATGC StuI 68 AGGCCT AGG^CCT 290 AGGCCT TfiI 278 GAATC G^A[AT]TC 323 GATTC TseI 252 GCAGC G^C[AT]GC 455 GCTGC ######################################### # 3 OR MORE CUT ENZYMES ( 34 enzymes) # ######################################### AluI AlwI AvaII BsaJI BsiYI BslI BsmFI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII EcoRII FokI HaeIII HinfI HphI MboI MboII MflI MnlI MvaI MwoI NdeII NlaIII PleI Sau3AI Sau96I ScrFI TspRI XhoII