################################## # NO CUT ENZYMES ( 175 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AflII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BalI BamHI BanI BbeI BbvCI BcgI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FseI FspI HaeII HapII HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII KasI KpnI KspI MfeI MluI MluNI MroI MscI MspA1I MspI MunI MvnI NaeI NarI NciI NcoI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmeI PpuMI PshBI Psp1406I PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI Sse8387I SspBI StuI StyI SwaI TaqI Tth111I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AfaI 171 GTAC GT^AC AflIII 517 ACACGT A^C[GA][CT]GT AluI 60 AGCT AG^CT AlwI 71 GGATC GGATC(4/5) BanII 163 GAGCCC G[GA]GC[CT]^C BbrPI 518 CACGTG CAC^GTG BbsI 155 GTCTTC GAAGAC(2/6) BciVI 454 GTATCC GTATCC(6/5) BclI 629 TGATCA T^GATCA BmyI 163 GAGCCC G[AGT]GC[ACT]^C BpmI 52 CTGGAG CTGGAG(16/14) BpuAI 155 GTCTTC GAAGAC(2/6) BsaAI 518 CACGTG [CT]AC^GT[GA] BsaBI 72 GATCTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaJI 46 CCAGGG C^C[ACGT][ACGT]GG BseRI 127 CTCCTC GAGGAG(10/8) BsiYI 339 CCTGGCAATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 339 CCTGGCAATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmAI 549 GAGAC GTCTC(1/5) Bsp1286I 163 GAGCCC G[AGT]GC[ACT]^C BstYI 71 GGATCT [GA]^GATC[CT] Cfr13I 5 GGCCC G^G[ACGT]CC DdeI 442 CTAAG C^T[ACGT]AG FbaI 629 TGATCA T^GATCA HaeIII 5 GGCC GG^CC HgaI 477 GCGTC GACGC(5/10) MaeII 519 ACGT A^CGT MamI 72 GATCTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 71 GGATCT [GA]^GATC[CT] MslI 813 CATTATCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NdeI 350 CATATG CA^TATG NlaIV 331 GGTTCC GG[ACGT]^[ACGT]CC NspI 122 GCATGC [GA]CATG^[CT] PmaCI 518 CACGTG CAC^GTG PmlI 518 CACGTG CAC^GTG PshAI 148 GACCACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 17 CTGCAG CTGCA^G RsaI 171 GTAC GT^AC Sau96I 5 GGCCC G^G[ACGT]CC SphI 122 GCATGC GCATG^C SspI 10 AATATT AAT^ATT Tsp45I 211 GTGAC ^GT[GC]AC XhoII 71 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 14 enzymes) # ################################### AciI 372 CCGC CCGC(-3/-1) 906 CCGC BfaI 410 CTAG C^TAG 619 CTAG BssKI 46 CCAGG ^CC[ACGT]GG 339 CCTGG BstNI 46 CCAGG CC^[AT]GG 339 CCTGG EarI 637 CTCTTC CTCTTC(1/4) 688 GAAGAG EcoRII 46 CCAGG ^CC[AT]GG 339 CCTGG Ksp632I 637 CTCTTC CTCTTC(1/4) 688 GAAGAG MaeI 410 CTAG C^TAG 619 CTAG MvaI 46 CCAGG CC^[AT]GG 339 CCTGG MwoI 272 GCAGTGTGGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 899 GCATTATCCGC PleI 622 GACTC GAGTC(4/5) 413 GAGTC ScrFI 46 CCAGG CC^[ACGT]GG 339 CCTGG TfiI 255 GAATC G^A[AT]TC 325 GAATC TspRI 67 CACTG CA[GC]TG[ACGT][ACGT]^ 273 CAGTG ######################################### # 3 OR MORE CUT ENZYMES ( 26 enzymes) # ######################################### BbvI BspHI BsrDI BsrI BstF5I Cac8I DpnII Fnu4HI FokI HinfI HphI ItaI MaeIII MboI MboII MnlI MseI NdeII NlaIII RcaI Sau3AI SfaNI SfcI Tru9I TseI Tsp509I