################################## # NO CUT ENZYMES ( 165 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AflII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspI AvaI AvaII AviII AvrII BanII BbeI BbrPI BcgI BciVI BcnI BfrI BlnI BlpI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaWI BseAI BsgI BsiEI BsiWI BsmBI BsmI BsoBI BspDI BspEI BspMI BsrBI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I CelII Cfr10I Cfr13I ClaI CpoI DraI DraII DraIII DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT14I EcoT22I FseI FspI HapII HgaI Hin1I HincII HindII HindIII HpaI HpaII KasI KpnI KspI MaeII MaeIII MamI MfeI MluI MroI MspA1I MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PstI PvuI PvuII RsrII SacI SacII SalI SapI Sau96I SbfI ScaI SexAI SfcI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I StuI StyI SwaI TaqI Tsp45I Tth111I TthHB8I Van91I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AciI 810 CCGC CCGC(-3/-1) Asp700I 67 GAAACACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 544 GAGCAC G[AT]GC[AT]^C BamHI 53 GGATCC G^GATCC BanI 849 GGTGCC G^G[CT][GA]CC BbsI 683 GTCTTC GAAGAC(2/6) BbvCI 714 CCTCAGC CCTCAGC(-5/-2) BbvI 164 GCAGC GCAGC(8/12) BclI 149 TGATCA T^GATCA BglI 196 GCCATGTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 896 AGATCT A^GATCT BpuAI 683 GTCTTC GAAGAC(2/6) BsaI 220 GGTCTC GGTCTC(1/5) BsaJI 57 CCCAGG C^C[ACGT][ACGT]GG BseRI 33 CTCCTC GAGGAG(10/8) BsiHKAI 544 GAGCAC G[AT]GC[AT]^C BsmAI 221 GTCTC GTCTC(1/5) BsmFI 711 GTCCC GGGAC(10/14) Bsp1407I 107 TGTACA T^GTACA BspHI 693 TCATGA T^CATGA BsrGI 107 TGTACA T^GTACA BsrI 21 CCAGT ACTGG(1/-1) BstXI 209 CCACTGATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CfoI 404 GCGC GCG^C DrdI 631 GACATCACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 949 GAAGAG CTCTTC(1/4) FauI 809 CCCGC CCCGC(4/6) FbaI 149 TGATCA T^GATCA Fnu4HI 164 GCAGC GC^[ACGT]GC HaeII 403 AGCGCC [GA]GCGC^[CT] HhaI 404 GCGC GCG^C HinP1I 404 GCGC G^CGC ItaI 164 GCAGC GC^[ACGT]GC Ksp632I 949 GAAGAG CTCTTC(1/4) MseI 463 TTAA T^TAA PshAI 704 GACTCAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 693 TCATGA T^CATGA SfiI 195 GGCCATGTTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmlI 706 CTCAAG C^T[CT][GA]AG SspBI 107 TGTACA T^GTACA Tru9I 463 TTAA T^TAA TseI 164 GCAGC G^C[AT]GC TspRI 210 CACTG CA[GC]TG[ACGT][ACGT]^ XcmI 206 CCACCACTGATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 67 GAAACACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AfaI 108 GTAC GT^AC 954 GTAC AflIII 721 ACATGT A^C[GA][CT]GT 732 ACATGT AluI 231 AGCT AG^CT 370 AGCT BmyI 544 GAGCAC G[AGT]GC[ACT]^C 805 GTGCCC Bsp1286I 544 GAGCAC G[AGT]GC[ACT]^C 805 GTGCCC BspLU11I 721 ACATGT A^CATGT 732 ACATGT BsrDI 642 CATTGC GCAATG(2/0) 355 GCAATG BssKI 58 CCAGG ^CC[ACGT]GG 192 CCTGG BstF5I 237 CATCC GGATG(2/0) 408 CATCC BstNI 58 CCAGG CC^[AT]GG 192 CCTGG BstYI 53 GGATCC [GA]^GATC[CT] 896 AGATCT Cac8I 292 GCCTGC GC[ACGT]^[ACGT]GC 574 GCCAGC EcoRII 58 CCAGG ^CC[AT]GG 192 CCTGG FokI 237 CATCC GGATG(9/13) 408 CATCC MflI 53 GGATCC [GA]^GATC[CT] 896 AGATCT MvaI 58 CCAGG CC^[AT]GG 192 CCTGG MwoI 196 GCCATGTTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 852 GCCACCAATGC NlaIV 53 GGATCC GG[ACGT]^[ACGT]CC 849 GGTGCC NspI 721 ACATGT [GA]CATG^[CT] 732 ACATGT RsaI 108 GTAC GT^AC 954 GTAC ScrFI 58 CCAGG CC^[ACGT]GG 192 CCTGG SfaNI 246 GATGC GCATC(5/9) 578 GCATC SspI 589 AATATT AAT^ATT 918 AATATT TfiI 254 GAATC G^A[AT]TC 516 GAATC Tsp509I 465 AATT ^AATT 525 AATT XhoII 53 GGATCC [GA]^GATC[CT] 896 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 22 enzymes) # ######################################### AlwI BalI BfaI BsiYI BslI DdeI DpnII EaeI HaeIII HinfI HphI MaeI MboI MboII MluNI MnlI MscI MslI NdeII NlaIII PleI Sau3AI