(Circular) (Six-base) MAPSORT of: pFN21AE6090.seq Check: 3608 from: 1 to: 5680 pFN21AE6090 5680 bp With 182 enzymes: SgfI * December 8, 2014 13:52 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3490 278 Size: 53 83 186 936 1954 2468 Fragments arranged by size: 2468 1954 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3023 1937 Size: 1086 4594 Acc65I G'GTAC_C Cuts at: 3001 3001 Size: 5680 AclI AA'CG_TT Cuts at: 1563 3807 4180 1563 Size: 2244 373 3063 Fragments arranged by size: 3063 2244 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3743 5127 5395 1632 Size: 2111 1384 268 1917 Fragments arranged by size: 2111 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5483 AflIII A'CryG_T Cuts at: 1203 2726 2737 4579 5556 1203 Size: 1523 11 1842 977 1327 Fragments arranged by size: 1842 1523 1327 977 11 AgeI A'CCGG_T Cuts at: 4511 4511 Size: 5680 AhdI GACnn_n'nnGTC Cuts at: 4409 4409 Size: 5680 AleI CACnn'nnGTG Cuts at: 1166 2219 2740 1166 Size: 1053 521 4106 Fragments arranged by size: 4106 1053 521 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5680 AlwNI CAG_nnn'CTG Cuts at: 3423 4995 3423 Size: 1572 4108 ApaLI G'TGCA_C Cuts at: 3737 4893 3737 Size: 1156 4524 ApoI r'AATT_y Cuts at: 1493 2989 3215 3367 5476 1493 Size: 1496 226 152 2109 1697 Fragments arranged by size: 2109 1697 1496 226 152 AseI AT'TA_AT Cuts at: 160 1032 4234 160 Size: 872 3202 1606 Fragments arranged by size: 3202 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5680 AvaI C'yCGr_G Cuts at: 1870 1943 3005 1870 Size: 73 1062 4545 Fragments arranged by size: 4545 1062 73 BamHI G'GATC_C Cuts at: 2058 3010 3377 2058 Size: 952 367 4361 Fragments arranged by size: 4361 952 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2854 3001 4456 618 Size: 325 217 627 1067 147 1455 1842 Fragments arranged by size: 1842 1455 1067 627 325 217 147 BanII G_rGCy'C Cuts at: 1391 2999 1391 Size: 1608 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2681 928 Size: 971 782 3927 Fragments arranged by size: 3927 971 782 BbvCI CC'TCA_GC Cuts at: 2720 2720 Size: 5680 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3871 3905 1903 Size: 34 1934 34 3678 Fragments arranged by size: 3678 1934 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3572 4782 3572 Size: 1210 4470 BclI T'GATC_A Cuts at: 1548 2154 1548 Size: 606 5074 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2207 4291 136 Size: 107 122 71 631 605 535 2084 1525 Fragments arranged by size: 2084 1525 631 605 535 122 107 71 BglII A'GATC_T Cuts at: 2901 5675 2901 Size: 2774 2906 BlpI GC'TnA_GC Cuts at: 3097 3097 Size: 5680 Bme1580I G_kGCm'C Cuts at: 1790 2814 3741 4897 1790 Size: 1024 927 1156 2573 Fragments arranged by size: 2573 1156 1024 927 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5680 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2020 3411 4369 Size: 416 53 83 399 24 603 1391 958 Cuts at: 4369 442 Size: 1753 Fragments arranged by size: 1753 1391 958 603 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5680 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4340 1239 Size: 3101 2579 Bpu10I CC'TnA_GC Cuts at: 2720 2720 Size: 5680 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2696 3805 4670 4968 2696 Size: 1109 865 298 3408 Fragments arranged by size: 3408 1109 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2231 4343 882 Size: 617 732 2112 2219 Fragments arranged by size: 2219 2112 732 617 BsaAI yAC'GTr Cuts at: 493 1204 3471 493 Size: 711 2267 2702 Fragments arranged by size: 2702 2267 711 BsaBI GATnn'nnATC Cuts at: 5674 5674 Size: 5680 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3487 3869 4504 275 Size: 53 83 186 936 1954 382 635 1451 Fragments arranged by size: 1954 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4113 4511 4785 4932 5343 4113 Size: 398 274 147 411 4450 Fragments arranged by size: 4450 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2024 2054 1178 Size: 30 165 30 621 30 4804 Fragments arranged by size: 4804 621 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2029 1186 Size: 843 4837 BseYI C'CCAG_C Cuts at: 2053 4883 5379 2053 Size: 2830 496 2354 Fragments arranged by size: 2830 2354 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5107 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3130 3891 4919 1540 Size: 275 186 1129 761 1028 2301 Fragments arranged by size: 2301 1129 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2553 3741 3826 4897 2553 Size: 1188 85 1071 3336 Fragments arranged by size: 3336 1188 1071 85 BsmI GAATG_Cn' Cuts at: 3191 3284 3191 Size: 93 5587 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2553 2814 2999 3741 3826 4897 1391 Size: 399 763 261 185 742 85 1071 2174 Fragments arranged by size: 2174 1071 763 742 399 261 185 85 BspEI T'CCGG_A Cuts at: 5343 5343 Size: 5680 BspHI T'CATG_A Cuts at: 2698 3569 5299 2698 Size: 871 1730 3079 Fragments arranged by size: 3079 1730 871 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3035 844 Size: 2191 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3567 1394 Size: 429 1744 3507 Fragments arranged by size: 3507 1744 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2367 2646 4175 4349 65 Size: 1183 1119 279 1529 174 1396 Fragments arranged by size: 1529 1396 1183 1119 279 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4324 4511 1700 Size: 110 2514 187 2869 Fragments arranged by size: 2869 2514 187 110 BsrGI T'GTAC_A Cuts at: 96 2112 96 Size: 2016 3664 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5680 BssSI C'ACGA_G Cuts at: 3740 4752 3740 Size: 1012 4668 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5680 BstBI TT'CG_AA Cuts at: 4484 4484 Size: 5680 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5680 BstXI CCAn_nnnn'nTGG Cuts at: 2221 2221 Size: 5680 BstYI r'GATC_y Cuts at: 1972 2058 2901 3010 3377 3762 3779 5231 5317 Size: 86 843 109 367 385 17 1452 86 Cuts at: 5317 5329 5675 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 843 385 367 346 109 86 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5128 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2777 487 Size: 683 56 1551 3390 Fragments arranged by size: 3390 1551 683 56 BtsI GCAGTG_nn' Cuts at: 3196 3990 4010 3196 Size: 794 20 4866 Fragments arranged by size: 4866 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5680 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5645 DraI TTT'AAA Cuts at: 2984 2984 Size: 5680 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2642 4687 809 Size: 507 1326 2045 1802 Fragments arranged by size: 2045 1802 1326 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2199 2208 2382 Size: 54 1425 50 275 18 369 9 174 Cuts at: 2382 2800 3127 4016 5645 8 Size: 418 327 889 1629 43 Fragments arranged by size: 1629 1425 889 418 369 327 275 174 54 50 43 18 9 EagI C'GGCC_G Cuts at: 1537 1812 3127 1537 Size: 275 1315 4090 Fragments arranged by size: 4090 1315 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2949 3610 1353 Size: 1596 661 3423 Fragments arranged by size: 3423 1596 661 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4267 4641 4787 387 Size: 1064 138 2678 374 146 1280 Fragments arranged by size: 2678 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3743 4340 5127 5395 1239 Size: 393 2111 597 787 268 1524 Fragments arranged by size: 2111 1524 787 597 393 268 EcoRI G'AATT_C Cuts at: 2989 3367 2989 Size: 378 5302 FspI TGC'GCA Cuts at: 4186 4186 Size: 5680 HaeII r_GCGC'y Cuts at: 1678 2412 4827 1678 Size: 734 2415 2531 Fragments arranged by size: 2531 2415 734 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2271 2303 4321 4353 4395 Size: 32 135 32 699 32 2018 32 42 Cuts at: 4395 4427 1373 Size: 32 2626 Fragments arranged by size: 2626 2018 699 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3024 3270 669 Size: 988 281 1086 246 3079 Fragments arranged by size: 3079 1086 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5680 HpaI GTT'AAC Cuts at: 3270 3270 Size: 5680 KpnI G_GTAC'C Cuts at: 3005 3005 Size: 5680 MfeI C'AATT_G Cuts at: 3279 3279 Size: 5680 MluI A'CGCG_T Cuts at: 5556 5556 Size: 5680 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4794 4978 1305 Size: 3489 184 2007 Fragments arranged by size: 3489 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2201 2210 2384 10 Size: 54 1425 343 369 9 174 3306 Fragments arranged by size: 3306 1425 369 343 174 54 9 MslI CAynn'nnrTG Cuts at: 518 1166 2219 2309 2336 2740 2742 3638 3997 Size: 648 1053 90 27 404 2 896 359 Cuts at: 3997 4156 518 Size: 159 2042 Fragments arranged by size: 2042 1053 896 648 404 359 159 90 27 2 MspA1I CmG'CkG Cuts at: 1606 3096 3773 4921 5166 1606 Size: 1490 677 1148 245 2120 Fragments arranged by size: 2120 1490 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5128 NdeI CA'TA_TG Cuts at: 387 387 Size: 5680 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5680 NotI GC'GGCC_GC Cuts at: 3127 3127 Size: 5680 NspI r_CATG'y Cuts at: 1132 1229 2730 2741 3038 4583 1132 Size: 97 1501 11 297 1545 2229 Fragments arranged by size: 2229 1545 1501 297 97 11 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5680 PciI A'CATG_T Cuts at: 2726 2737 4579 2726 Size: 11 1842 3827 Fragments arranged by size: 3827 1842 11 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5680 PmeI GTTT'AAAC Cuts at: 2984 2984 Size: 5680 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3749 3781 5262 5294 3749 Size: 32 1481 32 4135 Fragments arranged by size: 4135 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2713 1613 Size: 1100 4580 PsiI TTA'TAA Cuts at: 3250 3250 Size: 5680 PstI C_TGCA'G Cuts at: 830 3032 830 Size: 2202 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5680 SalI G'TCGA_C Cuts at: 1936 3022 1936 Size: 1086 4594 SbfI CC_TGCA'GG Cuts at: 3032 3032 Size: 5680 ScaI AGT'ACT Cuts at: 3928 5640 3928 Size: 1712 3968 SfcI C'TryA_G Cuts at: 826 1045 3028 4163 4844 5035 826 Size: 219 1983 1135 681 191 1471 Fragments arranged by size: 1983 1471 1135 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2207 2207 Size: 5680 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5680 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5680 SmaI CCC'GGG Cuts at: 1872 3007 1872 Size: 1135 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2711 3784 4685 4947 820 Size: 197 926 768 1073 901 262 1553 Fragments arranged by size: 1553 1073 926 901 768 262 197 SnaBI TAC'GTA Cuts at: 493 3471 493 Size: 2978 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5680 SphI G_CATG'C Cuts at: 1132 3038 1132 Size: 1906 3774 SspI AAT'ATT Cuts at: 5 52 2596 2925 3604 5 Size: 47 2544 329 679 2081 Fragments arranged by size: 2544 2081 679 329 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5680 StyI C'CwwG_G Cuts at: 513 1065 3119 513 Size: 552 2054 3074 Fragments arranged by size: 3074 2054 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2039 2321 3707 3724 3877 4062 4507 Size: 1255 459 282 1386 17 153 185 445 Cuts at: 4507 325 Size: 1498 Fragments arranged by size: 1498 1386 1255 459 445 282 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2112 3926 5638 Size: 274 80 33 51 1443 135 1814 1712 Cuts at: 5638 96 Size: 138 Fragments arranged by size: 1814 1712 1443 274 138 135 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2024 2056 5640 5672 2024 Size: 32 3584 32 2032 Fragments arranged by size: 3584 2032 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5680 XbaI T'CTAG_A Cuts at: 3016 3016 Size: 5680 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2218 2218 Size: 5680 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5680 XmaI C'CCGG_G Cuts at: 1870 3005 1870 Size: 1135 4545 XmnI GAAnn'nnTTC Cuts at: 2076 3809 2076 Size: 1733 3947 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3488 276 Size: 53 83 186 936 1954 2468 Fragments arranged by size: 2468 1954 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfoI SrfI SwaI