################################## # NO CUT ENZYMES ( 146 enzymes) # ################################## AatII AccI AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI ApaI ApaLI AscI AseI AsnI AspEI AspI AvaI AviII AvrII BalI BamHI BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BsaHI BsaWI BseAI BsiEI BsiWI BsmBI BsmFI BsmI BsoBI BspDI BspEI BspLU11I BsrBI BsrFI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I Cfr10I ClaI CpoI DraI DraII DraIII EagI Eam1105I EclXI Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HapII HgaI Hin1I HincII HindII HindIII HpaI HpaII KasI KspI MfeI MluI MluNI MroI MscI MspA1I MspI MunI NaeI NarI NciI NdeI NgoMIV NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RsrII SacI SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI Sse8387I SspI SwaI TaqI TfiI Tth111I TthHB8I XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### Acc65I 852 GGTACC G^GTACC AccII 814 CGCG CG^CG AcsI 899 AAATTC [GA]^AATT[CT] AlwI 83 GGATC GGATC(4/5) Aor51HI 497 AGCGCT AGC^GCT ApoI 899 AAATTC [GA]^AATT[CT] Asp700I 495 GAAGCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 852 GGTACC G^GTACC AvaII 175 GGACC G^G[AT]CC BanI 852 GGTACC G^G[CT][GA]CC BbvI 20 GCAGC GCAGC(8/12) BpmI 550 CTGGAG CTGGAG(16/14) BsaAI 373 TACGTG [CT]AC^GT[GA] BsaBI 279 GATTGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 70 GGTCTC GGTCTC(1/5) BseRI 466 CTCCTC GAGGAG(10/8) BsgI 618 CTGCAC GTGCAG(16/14) Bsp1407I 819 TGTACA T^GTACA BspHI 557 TCATGA T^CATGA BspMI 417 ACCTGC ACCTGC(4/8) BsrDI 687 CATTGC GCAATG(2/0) BsrGI 819 TGTACA T^GTACA BstUI 814 CGCG CG^CG BstXI 242 CCACTGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 83 GGATCT [GA]^GATC[CT] Cfr13I 175 GGACC G^G[ACGT]CC DrdI 400 GACTCCACCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 377 TGGCCG [CT]^GGCC[GA] Eco47III 497 AGCGCT AGC^GCT FauI 812 CCCGC CCCGC(4/6) Fnu4HI 20 GCAGC GC^[ACGT]GC HaeII 497 AGCGCT [GA]GCGC^[CT] ItaI 20 GCAGC GC^[ACGT]GC KpnI 852 GGTACC GGTAC^C MamI 279 GATTGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 83 GGATCT [GA]^GATC[CT] MvnI 814 CGCG CG^CG MwoI 240 GCCCACTGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NheI 201 GCTAGC G^CTAGC PflMI 519 CCATGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG RcaI 557 TCATGA T^CATGA Sau96I 175 GGACC G^G[ACGT]CC SfcI 879 CTACAG C^T[GA][CT]AG SphI 197 GCATGC GCATG^C SspBI 819 TGTACA T^GTACA StuI 219 AGGCCT AGG^CCT TseI 20 GCAGC G^C[AT]GC Van91I 519 CCATGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 63 TCTAGA T^CTAGA XhoII 83 GGATCT [GA]^GATC[CT] XmnI 495 GAAGCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AciI 369 CCGC CCGC(-3/-1) 813 CCGC AspHI 461 GTGCTC G[AT]GC[AT]^C 679 GTGCTC BanII 601 GGGCTC G[GA]GC[CT]^C 713 GAGCCC BbsI 327 GTCTTC GAAGAC(2/6) 542 GTCTTC BlpI 441 GCTAAGC GC^T[ACGT]AGC 681 GCTCAGC Bpu1102I 441 GCTAAGC GC^T[ACGT]AGC 681 GCTCAGC BpuAI 327 GTCTTC GAAGAC(2/6) 542 GTCTTC BsiHKAI 461 GTGCTC G[AT]GC[AT]^C 679 GTGCTC BssKI 549 CCTGG ^CC[ACGT]GG 699 CCAGG BstNI 549 CCTGG CC^[AT]GG 699 CCAGG CelII 441 GCTAAGC GC^T[ACGT]AGC 681 GCTCAGC CfoI 498 GCGC GCG^C 909 GCGC DpnII 84 GATC ^GATC 555 GATC EcoRII 549 CCTGG ^CC[AT]GG 699 CCAGG HhaI 498 GCGC GCG^C 909 GCGC HinP1I 498 GCGC G^CGC 909 GCGC MaeII 146 ACGT A^CGT 374 ACGT MaeIII 208 GTCAC ^GT[ACGT]AC 939 GTCAC MboI 84 GATC ^GATC 555 GATC MseI 14 TTAA T^TAA 917 TTAA MvaI 549 CCTGG CC^[AT]GG 699 CCAGG NcoI 75 CCATGG C^CATGG 171 CCATGG NdeII 84 GATC ^GATC 555 GATC NspI 197 GCATGC [GA]CATG^[CT] 421 GCATGT SapI 306 GCTCTTC GCTCTTC(1/4) 753 GCTCTTC Sau3AI 84 GATC ^GATC 555 GATC ScrFI 549 CCTGG CC^[ACGT]GG 699 CCAGG Tru9I 14 TTAA T^TAA 917 TTAA Tsp45I 208 GTCAC ^GT[GC]AC 939 GTCAC Tsp509I 6 AATT ^AATT 900 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 31 enzymes) # ######################################### AfaI AluI BfaI BmyI BsaJI BsiYI BslI BsmAI Bsp1286I BstF5I Cac8I DdeI DsaI EarI EcoT14I FokI HaeIII HinfI HphI Ksp632I MaeI MboII MnlI MslI NlaIII NlaIV PleI RsaI SfaNI StyI TspRI