(Circular) (Six-base) MAPSORT of: pFN21AE6070.seq Check: 3284 from: 1 to: 5668 pFN21AE6070 5668 bp With 182 enzymes: SgfI * December 8, 2014 13:51 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2431 2431 Size: 5668 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3478 278 Size: 53 83 186 936 1942 2468 Fragments arranged by size: 2468 1942 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3011 1937 Size: 1074 4594 Acc65I G'GTAC_C Cuts at: 2857 2989 2857 Size: 132 5536 AclI AA'CG_TT Cuts at: 1563 3795 4168 1563 Size: 2232 373 3063 Fragments arranged by size: 3063 2232 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2519 3731 5115 5383 1632 Size: 887 1212 1384 268 1917 Fragments arranged by size: 1917 1384 1212 887 268 AfeI AGC'GCT Cuts at: 2504 2504 Size: 5668 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5471 AflIII A'CryG_T Cuts at: 1203 4567 5544 1203 Size: 3364 977 1327 Fragments arranged by size: 3364 1327 977 AgeI A'CCGG_T Cuts at: 4499 4499 Size: 5668 AhdI GACnn_n'nnGTC Cuts at: 4397 4397 Size: 5668 AleI CACnn'nnGTG Cuts at: 1166 2950 1166 Size: 1784 3884 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2414 1184 Size: 1230 4438 AlwNI CAG_nnn'CTG Cuts at: 3411 4983 3411 Size: 1572 4096 ApaLI G'TGCA_C Cuts at: 3725 4881 3725 Size: 1156 4512 ApoI r'AATT_y Cuts at: 1493 2904 2977 3203 3355 5464 1493 Size: 1411 73 226 152 2109 1697 Fragments arranged by size: 2109 1697 1411 226 152 73 AseI AT'TA_AT Cuts at: 160 1032 4222 160 Size: 872 3190 1606 Fragments arranged by size: 3190 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5668 AvaI C'yCGr_G Cuts at: 1870 1943 2993 1870 Size: 73 1050 4545 Fragments arranged by size: 4545 1050 73 BamHI G'GATC_C Cuts at: 2998 3365 2998 Size: 367 5301 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2857 2989 4444 618 Size: 325 217 627 1070 132 1455 1842 Fragments arranged by size: 1842 1455 1070 627 325 217 132 BanII G_rGCy'C Cuts at: 1391 2610 2722 2987 1391 Size: 1219 112 265 4072 Fragments arranged by size: 4072 1219 265 112 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2325 2540 928 Size: 971 426 215 4056 Fragments arranged by size: 4056 971 426 215 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3859 3893 1903 Size: 34 1922 34 3678 Fragments arranged by size: 3678 1922 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3560 4770 3560 Size: 1210 4458 BclI T'GATC_A Cuts at: 1548 1548 Size: 5668 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4279 136 Size: 107 122 71 631 605 2607 1525 Fragments arranged by size: 2607 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5663 5663 Size: 5668 BlpI GC'TnA_GC Cuts at: 2447 2687 3085 2447 Size: 240 398 5030 Fragments arranged by size: 5030 398 240 Bme1580I G_kGCm'C Cuts at: 1790 3729 4885 1790 Size: 1939 1156 2573 Fragments arranged by size: 2573 1939 1156 BmgBI CAC'GTC Cuts at: 1318 2152 1318 Size: 834 4834 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3399 4357 442 Size: 416 53 83 399 24 1982 958 1753 Fragments arranged by size: 1982 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2210 1056 Size: 1154 4514 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2576 4328 1239 Size: 1337 1752 2579 Fragments arranged by size: 2579 1752 1337 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3793 4658 4956 3793 Size: 865 298 4505 Fragments arranged by size: 4505 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2081 4331 882 Size: 617 582 2250 2219 Fragments arranged by size: 2250 2219 617 582 BsaAI yAC'GTr Cuts at: 493 1204 2380 3459 493 Size: 711 1176 1079 2702 Fragments arranged by size: 2702 1176 1079 711 BsaBI GATnn'nnATC Cuts at: 2288 5662 2288 Size: 3374 2294 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3475 3857 4492 275 Size: 53 83 186 936 1942 382 635 1451 Fragments arranged by size: 1942 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4101 4499 4773 4920 5331 4101 Size: 398 274 147 411 4438 Fragments arranged by size: 4438 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2504 2534 1178 Size: 30 165 30 1101 30 4312 Fragments arranged by size: 4312 1101 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2462 1186 Size: 1276 4392 BseYI C'CCAG_C Cuts at: 2254 4871 5367 2254 Size: 2617 496 2555 Fragments arranged by size: 2617 2555 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2608 1163 Size: 573 872 4223 Fragments arranged by size: 4223 872 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3118 3879 4907 1540 Size: 275 186 1117 761 1028 2301 Fragments arranged by size: 2301 1117 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2470 2688 3729 3814 4885 2470 Size: 218 1041 85 1071 3253 Fragments arranged by size: 3253 1071 1041 218 85 BsmI GAATG_Cn' Cuts at: 3179 3272 3179 Size: 93 5575 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2470 2610 2688 2722 2987 3729 3814 Size: 399 680 140 78 34 265 742 85 Cuts at: 3814 4885 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 680 399 265 140 85 78 34 BspEI T'CCGG_A Cuts at: 5331 5331 Size: 5668 BspHI T'CATG_A Cuts at: 2562 3557 5287 2562 Size: 995 1730 2943 Fragments arranged by size: 2943 1730 995 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2431 3023 844 Size: 1587 592 3489 Fragments arranged by size: 3489 1587 592 BsrBI CCG'CTC Cuts at: 1394 1823 3555 1394 Size: 429 1732 3507 Fragments arranged by size: 3507 1732 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2691 4163 4337 65 Size: 1183 1443 1472 174 1396 Fragments arranged by size: 1472 1443 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4312 4499 1700 Size: 110 2502 187 2869 Fragments arranged by size: 2869 2502 187 110 BsrGI T'GTAC_A Cuts at: 96 2824 96 Size: 2728 2940 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5668 BssSI C'ACGA_G Cuts at: 3728 4740 3728 Size: 1012 4656 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5668 BstBI TT'CG_AA Cuts at: 4472 4472 Size: 5668 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5668 BstXI CCAn_nnnn'nTGG Cuts at: 2254 2254 Size: 5668 BstYI r'GATC_y Cuts at: 1972 2088 2998 3365 3750 3767 5219 5305 5317 Size: 116 910 367 385 17 1452 86 12 Cuts at: 5317 5663 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 910 385 367 346 116 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2080 2176 2628 513 Size: 552 1015 96 452 3553 Fragments arranged by size: 3553 1015 552 452 96 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2780 487 Size: 683 56 1554 3375 Fragments arranged by size: 3375 1554 683 56 BtsI GCAGTG_nn' Cuts at: 2240 2396 3184 3978 3998 2240 Size: 156 788 794 20 3910 Fragments arranged by size: 3910 794 788 156 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5668 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2138 2173 529 Size: 35 1574 35 4024 Fragments arranged by size: 4024 1574 35 35 DraI TTT'AAA Cuts at: 2972 2972 Size: 5668 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2411 4675 809 Size: 507 1095 2264 1802 Fragments arranged by size: 2264 1802 1095 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2382 3115 4004 Size: 54 1425 50 275 18 552 733 889 Cuts at: 4004 5633 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 733 552 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3115 1537 Size: 275 1303 4090 Fragments arranged by size: 4090 1303 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2318 2765 2837 3598 1353 Size: 965 447 72 761 3423 Fragments arranged by size: 3423 965 761 447 72 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4255 4629 4775 387 Size: 1064 138 2666 374 146 1280 Fragments arranged by size: 2666 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2519 2576 3731 4328 5115 5383 1239 Size: 393 887 57 1155 597 787 268 1524 Fragments arranged by size: 1524 1155 887 787 597 393 268 57 EcoRI G'AATT_C Cuts at: 2977 3355 2977 Size: 378 5290 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2058 2058 Size: 5668 FspI TGC'GCA Cuts at: 4174 4174 Size: 5668 HaeII r_GCGC'y Cuts at: 1678 2506 4815 1678 Size: 828 2309 2531 Fragments arranged by size: 2531 2309 828 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4309 4341 4383 4415 1373 Size: 32 135 32 2737 32 42 32 2626 Fragments arranged by size: 2737 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3012 3258 669 Size: 988 281 1074 246 3079 Fragments arranged by size: 3079 1074 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5668 HpaI GTT'AAC Cuts at: 3258 3258 Size: 5668 KpnI G_GTAC'C Cuts at: 2861 2993 2861 Size: 132 5536 MfeI C'AATT_G Cuts at: 3267 3267 Size: 5668 MluI A'CGCG_T Cuts at: 5544 5544 Size: 5668 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4782 4966 1305 Size: 3477 184 2007 Fragments arranged by size: 3477 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3846 Fragments arranged by size: 3846 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2425 2523 2601 2745 2771 2950 3626 Size: 648 1259 98 78 144 26 179 676 Cuts at: 3626 3985 4144 518 Size: 359 159 2042 Fragments arranged by size: 2042 1259 676 648 359 179 159 144 98 78 26 MspA1I CmG'CkG Cuts at: 1606 3084 3761 4909 5154 1606 Size: 1478 677 1148 245 2120 Fragments arranged by size: 2120 1478 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2080 2176 513 Size: 552 1015 96 4005 Fragments arranged by size: 4005 1015 552 96 NdeI CA'TA_TG Cuts at: 387 387 Size: 5668 NheI G'CTAG_C Cuts at: 1052 2206 1052 Size: 1154 4514 NotI GC'GGCC_GC Cuts at: 3115 3115 Size: 5668 NspI r_CATG'y Cuts at: 1132 1229 2206 2430 3026 4571 1132 Size: 97 977 224 596 1545 2229 Fragments arranged by size: 2229 1545 977 596 224 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5668 PciI A'CATG_T Cuts at: 4567 4567 Size: 5668 PflMI CCAn_nnn'nTGG Cuts at: 1108 2530 1108 Size: 1422 4246 PmeI GTTT'AAAC Cuts at: 2972 2972 Size: 5668 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3737 3769 5250 5282 3737 Size: 32 1481 32 4123 Fragments arranged by size: 4123 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5668 PsiI TTA'TAA Cuts at: 2939 3238 2939 Size: 299 5369 PstI C_TGCA'G Cuts at: 830 3020 830 Size: 2190 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5668 SalI G'TCGA_C Cuts at: 1936 3010 1936 Size: 1074 4594 SapI GCTCTTCn'nnn_ Cuts at: 2318 2765 2318 Size: 447 5221 SbfI CC_TGCA'GG Cuts at: 3020 3020 Size: 5668 ScaI AGT'ACT Cuts at: 3916 5628 3916 Size: 1712 3956 SfcI C'TryA_G Cuts at: 826 1045 2884 3016 4151 4832 5023 826 Size: 219 1839 132 1135 681 191 1471 Fragments arranged by size: 1839 1471 1135 681 219 191 132 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5668 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5668 SmaI CCC'GGG Cuts at: 1872 2995 1872 Size: 1123 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3772 4673 4935 820 Size: 197 926 1829 901 262 1553 Fragments arranged by size: 1829 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3459 493 Size: 2966 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5668 SphI G_CATG'C Cuts at: 1132 2206 3026 1132 Size: 1074 820 3774 Fragments arranged by size: 3774 1074 820 SspI AAT'ATT Cuts at: 5 52 3592 5 Size: 47 3540 2081 Fragments arranged by size: 3540 2081 47 StuI AGG'CCT Cuts at: 1517 2226 1517 Size: 709 4959 StyI C'CwwG_G Cuts at: 513 1065 2062 2080 2176 3107 513 Size: 552 997 18 96 931 3074 Fragments arranged by size: 3074 997 931 552 96 18 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2621 3695 3712 3865 4050 4495 325 Size: 1255 1041 1074 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1074 1041 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2159 2187 2752 Size: 274 80 33 51 1443 182 28 565 Cuts at: 2752 2824 3914 5626 96 Size: 72 1090 1712 138 Fragments arranged by size: 1712 1443 1090 565 274 182 138 80 72 51 33 28 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5628 5660 5628 Size: 32 5636 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5668 XbaI T'CTAG_A Cuts at: 2068 3004 2068 Size: 936 4732 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5668 XmaI C'CCGG_G Cuts at: 1870 2993 1870 Size: 1123 4545 XmnI GAAnn'nnTTC Cuts at: 2504 3797 2504 Size: 1293 4375 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3476 276 Size: 53 83 186 936 1942 2468 Fragments arranged by size: 2468 1942 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI