Restriction map for
pFN21AE6070
(Linear) (Six-base) MAPSORT of: KIEE6070.seq Check: 9840 from: 1 to: 963
KIEE6070.orf
With 182 enzymes: SgfI *
December 5, 2014 13:22 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 426 963
Size: 426 537
Acc65I G'GTAC_C
Cuts at: 0 852 963
Size: 852 111
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 514 963
Size: 514 449
AfeI AGC'GCT
Cuts at: 0 499 963
Size: 499 464
AleI CACnn'nnGTG
Cuts at: 0 945 963
Size: 945 18
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 409 963
Size: 409 554
ApoI r'AATT_y
Cuts at: 0 899 963
Size: 899 64
BanI G'GyrC_C
Cuts at: 0 852 963
Size: 852 111
BanII G_rGCy'C
Cuts at: 0 605 717 963
Size: 605 112 246
Fragments arranged by size:
605 246 112
BbsI GAAGACnn'nnnn_
Cuts at: 0 320 535 963
Size: 320 215 428
Fragments arranged by size:
428 320 215
BlpI GC'TnA_GC
Cuts at: 0 442 682 963
Size: 442 240 281
Fragments arranged by size:
442 281 240
BmgBI CAC'GTC
Cuts at: 0 147 963
Size: 147 816
BmtI G_CTAG'C
Cuts at: 0 205 963
Size: 205 758
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 571 963
Size: 571 392
BsaI GGTCTCn'nnnn_
Cuts at: 0 76 963
Size: 76 887
BsaAI yAC'GTr
Cuts at: 0 375 963
Size: 375 588
BsaBI GATnn'nnATC
Cuts at: 0 283 963
Size: 283 680
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 499 529 963
Size: 499 30 434
Fragments arranged by size:
499 434 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 457 963
Size: 457 506
BseYI C'CCAG_C
Cuts at: 0 249 963
Size: 249 714
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 603 963
Size: 603 360
BsiHKAI G_wGCw'C
Cuts at: 0 465 683 963
Size: 465 218 280
Fragments arranged by size:
465 280 218
Bsp1286I G_dGCh'C
Cuts at: 0 465 605 683 717 963
Size: 465 140 78 34 246
Fragments arranged by size:
465 246 140 78 34
BspHI T'CATG_A
Cuts at: 0 557 963
Size: 557 406
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 426 963
Size: 426 537
BsrDI GCAATG_nn'
Cuts at: 0 686 963
Size: 686 277
BsrGI T'GTAC_A
Cuts at: 0 819 963
Size: 819 144
BstXI CCAn_nnnn'nTGG
Cuts at: 0 249 963
Size: 249 714
BstYI r'GATC_y
Cuts at: 0 83 963
Size: 83 880
BtgI C'CryG_G
Cuts at: 0 75 171 623 963
Size: 75 96 452 340
Fragments arranged by size:
452 340 96 75
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 775 963
Size: 775 188
BtsI GCAGTG_nn'
Cuts at: 0 235 391 963
Size: 235 156 572
Fragments arranged by size:
572 235 156
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 133 168 963
Size: 133 35 795
Fragments arranged by size:
795 133 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 406 963
Size: 406 557
EaeI y'GGCC_r
Cuts at: 0 377 963
Size: 377 586
EarI CTCTTCn'nnn_
Cuts at: 0 313 760 832 963
Size: 313 447 72 131
Fragments arranged by size:
447 313 131 72
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 514 571 963
Size: 514 57 392
Fragments arranged by size:
514 392 57
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 53 963
Size: 53 910
HaeII r_GCGC'y
Cuts at: 0 501 963
Size: 501 462
KpnI G_GTAC'C
Cuts at: 0 856 963
Size: 856 107
MslI CAynn'nnrTG
Cuts at: 0 420 518 596 740 766 945 963
Size: 420 98 78 144 26 179 18
Fragments arranged by size:
420 179 144 98 78 26 18
NcoI C'CATG_G
Cuts at: 0 75 171 963
Size: 75 96 792
Fragments arranged by size:
792 96 75
NheI G'CTAG_C
Cuts at: 0 201 963
Size: 201 762
NspI r_CATG'y
Cuts at: 0 201 425 963
Size: 201 224 538
Fragments arranged by size:
538 224 201
PflMI CCAn_nnn'nTGG
Cuts at: 0 525 963
Size: 525 438
PsiI TTA'TAA
Cuts at: 0 934 963
Size: 934 29
SapI GCTCTTCn'nnn_
Cuts at: 0 313 760 963
Size: 313 447 203
Fragments arranged by size:
447 313 203
SfcI C'TryA_G
Cuts at: 0 879 963
Size: 879 84
SphI G_CATG'C
Cuts at: 0 201 963
Size: 201 762
StuI AGG'CCT
Cuts at: 0 221 963
Size: 221 742
StyI C'CwwG_G
Cuts at: 0 57 75 171 963
Size: 57 18 96 792
Fragments arranged by size:
792 96 57 18
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 616 963
Size: 616 347
TatI w'GTAC_w
Cuts at: 0 154 182 747 819 963
Size: 154 28 565 72 144
Fragments arranged by size:
565 154 144 72 28
XbaI T'CTAG_A
Cuts at: 0 63 963
Size: 63 900
XmnI GAAnn'nnTTC
Cuts at: 0 499 963
Size: 499 464
Enzymes that do cut:
AarI Acc65I AcuI AfeI AleI AlfI ApoI BanI
BanII BbsI BlpI BmgBI BmtI BpmI BsaI BsaAI
BsaBI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspHI
BspMI BsrDI BsrGI BstXI BstYI BtgI BtgZI BtsI
CspCI DrdI EaeI EarI Eco57MI FalI HaeII KpnI
MslI NcoI NheI NspI PflMI PsiI SapI SfcI
SphI StuI StyI TaqII TatI XbaI XmnI
Enzymes that do not cut:
AatII AccI AclI AflII AflIII AgeI AhdI AloI
AlwNI ApaI ApaLI AscI AseI AsiSI AvaI AvrII
BaeI BamHI BbeI BbvCI BcgI BciVI BclI BfrBI
BglI BglII Bme1580I BmrI BplI Bpu10I BpuEI BsaHI
BsaWI BsiEI BsiWI BsmI BsmBI BspEI BsrBI BsrFI
BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I ClaI
DraI DraIII EagI EciI EcoICRI EcoNI EcoO109I EcoRI
EcoRV FseI FspI FspAI Hin4I HincII HindIII HpaI
KasI MfeI MluI MmeI MscI MspA1I NaeI NarI
NdeI NgoMIV NotI NruI NsiI PacI PasI PciI
PfoI PmeI PmlI PpiI PpuMI PshAI PspOMI PspXI
PsrI PstI PvuI PvuII RsrII SacI SacII SalI
SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SmlI SnaBI SpeI SrfI SspI SwaI TstI
Tth111I XcmI XhoI XmaI ZraI