################################## # NO CUT ENZYMES ( 162 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BalI BbeI BbrPI BbvCI BcgI BclI BcnI BlnI BlpI BpmI Bpu1102I BsaAI BsaHI BsaI BsaWI BseAI BsiEI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraIII DrdI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRV FauI FbaI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HpaI HpaII KasI KpnI Ksp632I KspI MaeII MaeIII MfeI MluI MluNI MroI MscI MspA1I MspI MunI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI Tsp45I Tth111I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AspHI 739 GTGCTC G[AT]GC[AT]^C AvaII 360 GGACC G^G[AT]CC BamHI 47 GGATCC G^GATCC BanI 843 GGTGCC G^G[CT][GA]CC BbvI 772 GCTGC GCAGC(8/12) BciVI 546 GGATAC GTATCC(6/5) BfaI 410 CTAG C^TAG BglI 190 GCCATGTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 902 AGATCT A^GATCT BsaBI 42 GATTGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseRI 225 CTCCTC GAGGAG(10/8) BsgI 126 CTGCAC GTGCAG(16/14) BsiHKAI 739 GTGCTC G[AT]GC[AT]^C BsmI 455 GCATTC GAATGC(1/-1) BspHI 165 TCATGA T^CATGA BspMI 104 ACCTGC ACCTGC(4/8) BsrI 57 ACTGG ACTGG(1/-1) BstF5I 84 CATCC GGATG(2/0) BstXI 551 CCATGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DraII 704 AGGCCCT [GA]G^G[ACGT]CC[CT] DsaI 266 CCATGG C^C[GA][CT]GG EcoO109I 704 AGGCCCT [GA]G^G[ACGT]CC[CT] EcoRI 301 GAATTC G^AATTC EcoT14I 266 CCATGG C^C[AT][AT]GG EcoT22I 778 ATGCAT ATGCA^T FokI 84 CATCC GGATG(9/13) HindIII 28 AAGCTT A^AGCTT MaeI 410 CTAG C^TAG MamI 42 GATTGGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NcoI 266 CCATGG C^CATGG NsiI 778 ATGCAT ATGCA^T NspI 63 ACATGC [GA]CATG^[CT] PleI 882 GACTC GAGTC(4/5) PshAI 579 GACCAATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 165 TCATGA T^CATGA ScaI 339 AGTACT AGT^ACT SspI 500 AATATT AAT^ATT StyI 266 CCATGG C^C[AT][AT]GG TaqI 885 TCGA T^CGA TseI 772 GCTGC G^C[AT]GC TthHB8I 885 TCGA T^CGA XcmI 758 CCATCATCACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AciI 363 CCGC CCGC(-3/-1) 847 CCGC AcsI 272 GAATTT [GA]^AATT[CT] 301 GAATTC AluI 29 AGCT AG^CT 557 AGCT ApoI 272 GAATTT [GA]^AATT[CT] 301 GAATTC BanII 154 GAGCCC G[GA]GC[CT]^C 169 GAGCCC BsmAI 202 GTCTC GTCTC(1/5) 721 GTCTC BsmFI 432 GGGAC GGGAC(10/14) 219 GTCCC BsrDI 603 CATTGC GCAATG(2/0) 678 CATTGC BssKI 52 CCAGG ^CC[ACGT]GG 768 CCTGG BstNI 52 CCAGG CC^[AT]GG 768 CCTGG BstYI 47 GGATCC [GA]^GATC[CT] 902 AGATCT EcoRII 52 CCAGG ^CC[AT]GG 768 CCTGG Fnu4HI 772 GCTGC GC^[ACGT]GC 846 GCCGC ItaI 772 GCTGC GC^[ACGT]GC 846 GCCGC MflI 47 GGATCC [GA]^GATC[CT] 902 AGATCT MslI 321 CACTGTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 727 CACATCTGTG MvaI 52 CCAGG CC^[AT]GG 768 CCTGG NlaIV 47 GGATCC GG[ACGT]^[ACGT]CC 843 GGTGCC ScrFI 52 CCAGG CC^[ACGT]GG 768 CCTGG SfaNI 682 GCATC GCATC(5/9) 780 GCATC TspRI 321 CACTG CA[GC]TG[ACGT][ACGT]^ 632 CACTG XhoII 47 GGATCC [GA]^GATC[CT] 902 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 32 enzymes) # ######################################### AfaI AflII AlwI BbsI BfrI BmyI BpuAI BsaJI BsiYI BslI Bsp1286I Cac8I Cfr13I DdeI DpnII HaeIII HinfI HphI MboI MboII MnlI MseI MwoI NdeII NlaIII RsaI Sau3AI Sau96I SmlI TfiI Tru9I Tsp509I