################################## # NO CUT ENZYMES ( 162 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BamHI BanI BanII BbeI BbrPI BbvCI BcgI BciVI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiWI BsmBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraII DraIII EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HpaI KasI KpnI KspI MaeI MamI MfeI MluI MroI MunI MvnI MwoI NaeI NarI NdeI NgoMIV NheI NlaIV NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfaNI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI Sse8387I SspBI StuI SwaI TaqI Tth111I TthHB8I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AfaI 755 GTAC GT^AC AvaI 353 CCCGGG C^[CT]CG[GA]G AvaII 507 GGTCC G^G[AT]CC BalI 914 TGGCCA TGG^CCA BpmI 415 CTGGAG CTGGAG(16/14) BseRI 124 CTCCTC GAGGAG(10/8) BsgI 824 GTGCAG GTGCAG(16/14) BsmFI 681 GGGAC GGGAC(10/14) BsmI 133 GCATTC GAATGC(1/-1) BsoBI 353 CCCGGG C^[CT]CG[GA]G BsrDI 195 CATTGC GCAATG(2/0) BsrI 175 CCAGT ACTGG(1/-1) BssSI 601 CACGAG CACGAG(-5/-1) BstYI 164 GGATCT [GA]^GATC[CT] DraI 367 TTTAAA TTT^AAA DrdI 499 GACAGGATGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 914 TGGCCA [CT]^GGCC[GA] EarI 877 CTCTTC CTCTTC(1/4) HapII 354 CCGG C^CGG HindIII 371 AAGCTT A^AGCTT HpaII 354 CCGG C^CGG HphI 513 GGTGA GGTGA(8/7) Ksp632I 877 CTCTTC CTCTTC(1/4) MaeII 716 ACGT A^CGT MflI 164 GGATCT [GA]^GATC[CT] MluNI 914 TGGCCA TGG^CCA MscI 914 TGGCCA TGG^CCA MspI 354 CCGG C^CGG NspI 399 GCATGC [GA]CATG^[CT] PflMI 656 CCAAACCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG RsaI 755 GTAC GT^AC SfcI 903 CTGTAG C^T[GA][CT]AG SmaI 353 CCCGGG CCC^GGG SphI 399 GCATGC GCATG^C TfiI 33 GAATC G^A[AT]TC Tsp509I 40 AATT ^AATT Van91I 656 CCAAACCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 194 CCATTGCCCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoII 164 GGATCT [GA]^GATC[CT] XmaCI 353 CCCGGG C^CCGGG XmaI 353 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AspHI 152 GTGCTC G[AT]GC[AT]^C 898 GTGCTC BbvI 286 GCAGC GCAGC(8/12) 1002 GCTGC BclI 638 TGATCA T^GATCA 851 TGATCA BcnI 353 CCCGG CC^[GC]GG 354 CCGGG BmyI 152 GTGCTC G[AGT]GC[ACT]^C 898 GTGCTC BsiHKAI 152 GTGCTC G[AT]GC[AT]^C 898 GTGCTC BsmAI 246 GAGAC GTCTC(1/5) 185 GTCTC Bsp1286I 152 GTGCTC G[AGT]GC[ACT]^C 898 GTGCTC BstF5I 613 CATCC GGATG(2/0) 503 GGATG Cfr13I 507 GGTCC G^G[ACGT]CC 774 GGCCC DsaI 80 CCATGG C^C[GA][CT]GG 437 CCATGG FbaI 638 TGATCA T^GATCA 851 TGATCA Fnu4HI 286 GCAGC GC^[ACGT]GC 1002 GCTGC FokI 613 CATCC GGATG(9/13) 503 GGATG ItaI 286 GCAGC GC^[ACGT]GC 1002 GCTGC MslI 381 CATTCAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 817 CACATCTGTG MspA1I 287 CAGCTG C[AC]G^C[GT]G 1000 CAGCTG NciI 353 CCCGG CC^[GC]GG 354 CCGGG NcoI 80 CCATGG C^CATGG 437 CCATGG PleI 248 GACTC GAGTC(4/5) 418 GAGTC PvuII 287 CAGCTG CAG^CTG 1000 CAGCTG Sau96I 507 GGTCC G^G[ACGT]CC 774 GGCCC SspI 87 AATATT AAT^ATT 919 AATATT TseI 286 GCAGC G^C[AT]GC 1002 GCTGC ######################################### # 3 OR MORE CUT ENZYMES ( 31 enzymes) # ######################################### AciI AluI AlwI BbsI BpuAI BsaJI BsiYI BslI BssKI BstNI Cac8I DdeI DpnII EcoRII EcoT14I HaeIII HinfI MaeIII MboI MboII MnlI MseI MvaI NdeII NlaIII Sau3AI ScrFI StyI Tru9I Tsp45I TspRI