################################## # NO CUT ENZYMES ( 165 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BamHI BanI BanII BbeI BbrPI BbvI BcgI BciVI BcnI BfaI BfrI BglI BglII BlnI BpmI BsaAI BsaHI BsaI BsaWI BseAI BseRI BsiEI BsiWI BspDI BspEI BspHI BspLU11I BsrBI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I Cfr10I ClaI CpoI DraI DraII DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI Fnu4HI FseI FspI HaeII HapII HgaI Hin1I HincII HindII HindIII HpaI HpaII HphI ItaI KasI KpnI KspI MaeI MaeII MfeI MluI MroI MspA1I MspI MunI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspI SwaI TseI Tsp45I Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AciI 744 GCGG CCGC(-3/-1) Asp700I 747 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspHI 898 GTGCTC G[AT]GC[AT]^C AvaI 308 CCCGAG C^[CT]CG[GA]G BbvCI 717 CCTCAGC CCTCAGC(-5/-2) BclI 701 TGATCA T^GATCA BlpI 61 GCTGAGC GC^T[ACGT]AGC BmyI 898 GTGCTC G[AGT]GC[ACT]^C Bpu1102I 61 GCTGAGC GC^T[ACGT]AGC BsaBI 120 GATCTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsiHKAI 898 GTGCTC G[AT]GC[AT]^C BsmBI 156 CGTCTC CGTCTC(1/5) BsmFI 261 GTCCC GGGAC(10/14) BsmI 89 GCATTC GAATGC(1/-1) BsoBI 308 CCCGAG C^[CT]CG[GA]G Bsp1286I 898 GTGCTC G[AGT]GC[ACT]^C Bsp1407I 268 TGTACA T^GTACA BspMI 760 ACCTGC ACCTGC(4/8) BsrGI 268 TGTACA T^GTACA BssKI 94 CCAGG ^CC[ACGT]GG BstNI 94 CCAGG CC^[AT]GG BstXI 825 CCATCGCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 119 GGATCT [GA]^GATC[CT] Cac8I 328 GCCTGC GC[ACGT]^[ACGT]GC CelII 61 GCTGAGC GC^T[ACGT]AGC CfoI 646 GCGC GCG^C DsaI 392 CCATGG C^C[GA][CT]GG EarI 736 GAAGAG CTCTTC(1/4) EcoRII 94 CCAGG ^CC[AT]GG FbaI 701 TGATCA T^GATCA HhaI 646 GCGC GCG^C HinP1I 646 GCGC G^CGC Ksp632I 736 GAAGAG CTCTTC(1/4) MaeIII 422 GTTAC ^GT[ACGT]AC MamI 120 GATCTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 119 GGATCT [GA]^GATC[CT] MvaI 94 CCAGG CC^[AT]GG NcoI 392 CCATGG C^CATGG NlaIV 200 GGAGCC GG[ACGT]^[ACGT]CC NspI 482 GCATGT [GA]CATG^[CT] PleI 373 GAGTC GAGTC(4/5) PstI 546 CTGCAG CTGCA^G ScaI 113 AGTACT AGT^ACT ScrFI 94 CCAGG CC^[ACGT]GG SspBI 268 TGTACA T^GTACA StuI 96 AGGCCT AGG^CCT TaqI 708 TCGA T^CGA TfiI 936 GATTC G^A[AT]TC TthHB8I 708 TCGA T^CGA XhoII 119 GGATCT [GA]^GATC[CT] XmnI 747 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 10 enzymes) # ################################### AluI 256 AGCT AG^CT 992 AGCT BbsI 18 GTCTTC GAAGAC(2/6) 292 GTCTTC BpuAI 18 GTCTTC GAAGAC(2/6) 292 GTCTTC BsgI 606 CTGCAC GTGCAG(16/14) 762 CTGCAC HinfI 373 GAGTC G^A[ACGT]TC 936 GATTC MseI 929 TTAA T^TAA 961 TTAA SfaNI 629 GCATC GCATC(5/9) 680 GCATC SfcI 546 CTGCAG C^T[GA][CT]AG 659 CTACAG Tru9I 929 TTAA T^TAA 961 TTAA Tsp509I 462 AATT ^AATT 963 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 32 enzymes) # ######################################### AfaI AlwI AvaII BalI BsaJI BsiYI BslI BsmAI BsrDI BsrI BstF5I Cfr13I DdeI DpnII EaeI EcoT14I FokI HaeIII MboI MboII MluNI MnlI MscI MslI MwoI NdeII NlaIII RsaI Sau3AI Sau96I StyI TspRI