Restriction map for
pFN21AE5488
(Circular) (Six-base) MAPSORT of: pFN21AE5488.seq Check: 3049 from: 1 to: 5731
pFN21AE5488 5731 bp
With 182 enzymes: SgfI *
January 30, 2014 17:21 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2774 2774
Size: 5731
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3541 278
Size: 53 83 186 936 2005 2468
Fragments arranged by size:
2468 2005 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3074 1937
Size: 1137 4594
Acc65I G'GTAC_C
Cuts at: 3052 3052
Size: 5731
AclI AA'CG_TT
Cuts at: 1563 3858 4231 1563
Size: 2295 373 3063
Fragments arranged by size:
3063 2295 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3794 5178 5446 1632
Size: 2162 1384 268 1917
Fragments arranged by size:
2162 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5534
AflIII A'CryG_T
Cuts at: 1203 4630 5607 1203
Size: 3427 977 1327
Fragments arranged by size:
3427 1327 977
AgeI A'CCGG_T
Cuts at: 4562 4562
Size: 5731
AhdI GACnn_n'nnGTC
Cuts at: 4460 4460
Size: 5731
AleI CACnn'nnGTG
Cuts at: 1166 2781 1166
Size: 1615 4116
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5731
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2193 2225 2193
Size: 32 5699
AlwNI CAG_nnn'CTG
Cuts at: 3474 5046 3474
Size: 1572 4159
ApaLI G'TGCA_C
Cuts at: 3788 4944 3788
Size: 1156 4575
ApoI r'AATT_y
Cuts at: 1493 3040 3266 3418 5527 1493
Size: 1547 226 152 2109 1697
Fragments arranged by size:
2109 1697 1547 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4285 160
Size: 872 3253 1606
Fragments arranged by size:
3253 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5731
AvaI C'yCGr_G
Cuts at: 1870 1943 2313 3056 1870
Size: 73 370 743 4545
Fragments arranged by size:
4545 743 370 73
BamHI G'GATC_C
Cuts at: 3061 3428 3061
Size: 367 5364
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3052 4507 618
Size: 325 217 627 1265 1455 1842
Fragments arranged by size:
1842 1455 1265 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3050 1391
Size: 1659 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2016 2290 928
Size: 971 117 274 4369
Fragments arranged by size:
4369 971 274 117
BbvCI CC'TCA_GC
Cuts at: 2723 2723
Size: 5731
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3922 3956 1903
Size: 34 1985 34 3678
Fragments arranged by size:
3678 1985 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3623 4833 3623
Size: 1210 4521
BclI T'GATC_A
Cuts at: 1548 2706 1548
Size: 1158 4573
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4342 136
Size: 107 122 71 631 605 2670 1525
Fragments arranged by size:
2670 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5726 5726
Size: 5731
BlpI GC'TnA_GC
Cuts at: 2067 3148 2067
Size: 1081 4650
Bme1580I G_kGCm'C
Cuts at: 1790 3792 4948 1790
Size: 2002 1156 2573
Fragments arranged by size:
2573 2002 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5731
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2110 3462 4420
Size: 416 53 83 399 24 693 1352 958
Cuts at: 4420 442
Size: 1753
Fragments arranged by size:
1753 1352 958 693 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5731
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4391 1239
Size: 3152 2579
Bpu10I CC'TnA_GC
Cuts at: 2258 2723 2258
Size: 465 5266
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3856 4721 5019 3856
Size: 865 298 4568
Fragments arranged by size:
4568 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4394 882
Size: 617 2895 2219
Fragments arranged by size:
2895 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3522 493
Size: 711 2318 2702
Fragments arranged by size:
2702 2318 711
BsaBI GATnn'nnATC
Cuts at: 2129 5725 2129
Size: 3596 2135
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3538 3920 4555 275
Size: 53 83 186 936 2005 382 635 1451
Fragments arranged by size:
2005 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4164 4562 4836 4983 5394 4164
Size: 398 274 147 411 4501
Fragments arranged by size:
4501 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2111 2141 2193 2223 1178
Size: 30 165 30 708 30 52 30 4686
Fragments arranged by size:
4686 708 165 52 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5731
BseYI C'CCAG_C
Cuts at: 4934 5430 4934
Size: 496 5235
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2596 2752 1163
Size: 573 860 156 4142
Fragments arranged by size:
4142 860 573 156
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3181 3942 4970 1540
Size: 275 186 1180 761 1028 2301
Fragments arranged by size:
2301 1180 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2907 3792 3877 4948 2907
Size: 885 85 1071 3690
Fragments arranged by size:
3690 1071 885 85
BsmI GAATG_Cn'
Cuts at: 2094 3242 3335 2094
Size: 1148 93 4490
Fragments arranged by size:
4490 1148 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2167 2167
Size: 5731
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2907 3050 3792 3877 4948 1391
Size: 399 1117 143 742 85 1071 2174
Fragments arranged by size:
2174 1117 1071 742 399 143 85
BspEI T'CCGG_A
Cuts at: 5394 5394
Size: 5731
BspHI T'CATG_A
Cuts at: 3620 5350 3620
Size: 1730 4001
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2774 3086 844
Size: 1930 312 3489
Fragments arranged by size:
3489 1930 312
BsrBI CCG'CTC
Cuts at: 1394 1823 3618 1394
Size: 429 1795 3507
Fragments arranged by size:
3507 1795 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2061 2337 2727 4226 4400 65
Size: 1183 813 276 390 1499 174 1396
Fragments arranged by size:
1499 1396 1183 813 390 276 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4375 4562 1700
Size: 110 2565 187 2869
Fragments arranged by size:
2869 2565 187 110
BsrGI T'GTAC_A
Cuts at: 96 2273 96
Size: 2177 3554
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5731
BssSI C'ACGA_G
Cuts at: 3791 4803 3791
Size: 1012 4719
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5731
BstBI TT'CG_AA
Cuts at: 4535 4535
Size: 5731
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5731
BstXI CCAn_nnnn'nTGG
Cuts at: 2837 2837
Size: 5731
BstYI r'GATC_y
Cuts at: 1972 2124 3061 3428 3813 3830 5282 5368 5380
Size: 152 937 367 385 17 1452 86 12
Cuts at: 5380 5726 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 937 385 367 346 152 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2397 513
Size: 552 1332 3847
Fragments arranged by size:
3847 1332 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2816 487
Size: 683 56 1590 3402
Fragments arranged by size:
3402 1590 683 56
BtsI GCAGTG_nn'
Cuts at: 3247 4041 4061 3247
Size: 794 20 4917
Fragments arranged by size:
4917 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5731
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5696
DraI TTT'AAA
Cuts at: 3035 3035
Size: 5731
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4738 809
Size: 507 3422 1802
Fragments arranged by size:
3422 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2394 2418 2839
Size: 54 1425 50 275 18 564 24 421
Cuts at: 2839 2874 3178 4067 5696 8
Size: 35 304 889 1629 43
Fragments arranged by size:
1629 1425 889 564 421 304 275 54
50 43 35 24 18
EagI C'GGCC_G
Cuts at: 1537 1812 3178 1537
Size: 275 1366 4090
Fragments arranged by size:
4090 1366 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2736 3661 1353
Size: 1383 925 3423
Fragments arranged by size:
3423 1383 925
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2764 4318 4692 4838 387
Size: 1064 138 1175 1554 374 146 1280
Fragments arranged by size:
1554 1280 1175 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3794 4391 5178 5446 1239
Size: 393 2162 597 787 268 1524
Fragments arranged by size:
2162 1524 787 597 393 268
EcoRI G'AATT_C
Cuts at: 3040 3418 3040
Size: 378 5353
FspI TGC'GCA
Cuts at: 4237 4237
Size: 5731
HaeII r_GCGC'y
Cuts at: 1678 4878 1678
Size: 3200 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2706 2738 4372 4404 4446
Size: 32 135 32 1134 32 1634 32 42
Cuts at: 4446 4478 1373
Size: 32 2626
Fragments arranged by size:
2626 1634 1134 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3075 3321 669
Size: 988 281 1137 246 3079
Fragments arranged by size:
3079 1137 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5731
HpaI GTT'AAC
Cuts at: 3321 3321
Size: 5731
KpnI G_GTAC'C
Cuts at: 3056 3056
Size: 5731
MfeI C'AATT_G
Cuts at: 3330 3330
Size: 5731
MluI A'CGCG_T
Cuts at: 5607 5607
Size: 5731
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2626 4845 5029 1305
Size: 1321 2219 184 2007
Fragments arranged by size:
2219 2007 1321 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2396 2420 2841 2876 10
Size: 54 1425 343 564 24 421 35 2865
Fragments arranged by size:
2865 1425 564 421 343 54 35 24
MslI CAynn'nnrTG
Cuts at: 518 1166 2781 2835 2870 3689 4048 4207 518
Size: 648 1615 54 35 819 359 159 2042
Fragments arranged by size:
2042 1615 819 648 359 159 54 35
MspA1I CmG'CkG
Cuts at: 1606 3147 3824 4972 5217 1606
Size: 1541 677 1148 245 2120
Fragments arranged by size:
2120 1541 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2397 513
Size: 552 1332 3847
Fragments arranged by size:
3847 1332 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5731
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5731
NotI GC'GGCC_GC
Cuts at: 3178 3178
Size: 5731
NspI r_CATG'y
Cuts at: 1132 1229 2491 3089 4634 1132
Size: 97 1262 598 1545 2229
Fragments arranged by size:
2229 1545 1262 598 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5731
PciI A'CATG_T
Cuts at: 4630 4630
Size: 5731
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5731
PmeI GTTT'AAAC
Cuts at: 3035 3035
Size: 5731
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2193 2225 3800 3832 5313 5345 2193
Size: 32 1575 32 1481 32 2579
Fragments arranged by size:
2579 1575 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5731
PsiI TTA'TAA
Cuts at: 3301 3301
Size: 5731
PstI C_TGCA'G
Cuts at: 830 2555 3083 830
Size: 1725 528 3478
Fragments arranged by size:
3478 1725 528
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5731
SalI G'TCGA_C
Cuts at: 1936 3073 1936
Size: 1137 4594
SbfI CC_TGCA'GG
Cuts at: 3083 3083
Size: 5731
ScaI AGT'ACT
Cuts at: 2120 3979 5691 2120
Size: 1859 1712 2160
Fragments arranged by size:
2160 1859 1712
SfcI C'TryA_G
Cuts at: 826 1045 2551 2664 3079 4214 4895 5086 826
Size: 219 1506 113 415 1135 681 191 1471
Fragments arranged by size:
1506 1471 1135 681 415 219 191 113
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5731
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5731
SmaI CCC'GGG
Cuts at: 1872 3058 1872
Size: 1186 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3835 4736 4998 820
Size: 197 926 1892 901 262 1553
Fragments arranged by size:
1892 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3522 493
Size: 3029 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5731
SphI G_CATG'C
Cuts at: 1132 3089 1132
Size: 1957 3774
SspI AAT'ATT
Cuts at: 5 52 3655 5
Size: 47 3603 2081
Fragments arranged by size:
3603 2081 47
StuI AGG'CCT
Cuts at: 1517 2103 1517
Size: 586 5145
StyI C'CwwG_G
Cuts at: 513 1065 2290 2397 2638 3170 513
Size: 552 1225 107 241 532 3074
Fragments arranged by size:
3074 1225 552 532 241 107
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2035 2129 3758 3775 3928 4113 4558
Size: 1255 455 94 1629 17 153 185 445
Cuts at: 4558 325
Size: 1498
Fragments arranged by size:
1629 1498 1255 455 445 185 153 94
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2118 2223 2273
Size: 274 80 33 51 1443 141 105 50
Cuts at: 2273 3977 5689 96
Size: 1704 1712 138
Fragments arranged by size:
1712 1704 1443 274 141 138 105 80
51 50 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5691 5723 5691
Size: 32 5699
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5731
XbaI T'CTAG_A
Cuts at: 3067 3067
Size: 5731
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5731
XmaI C'CCGG_G
Cuts at: 1870 3056 1870
Size: 1186 4545
XmnI GAAnn'nnTTC
Cuts at: 2756 3860 2756
Size: 1104 4627
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3539 276
Size: 53 83 186 936 2005 2468
Fragments arranged by size:
2468 2005 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PmeI PpiI PshAI PsiI PstI
PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI
PvuII RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI XcmI