################################## # NO CUT ENZYMES ( 161 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AflII AgeI AhdI Alw44I AlwI AlwNI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AviII AvrII BamHI BanI BbeI BbrPI BbsI BbvCI BcgI BciVI BcnI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiWI BsmAI BsmBI BsmI BspDI BspEI BspLU11I BspMI BsrBI BsrDI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I CelII CfoI ClaI CpoI DraI DraII DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I EcoO109I EcoO65I EcoRI EcoRV EcoT22I FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HpaI KasI KpnI KspI MaeIII MamI MfeI MflI MluI MroI MseI MspA1I MunI MvnI NarI NciI NcoI NdeI NheI NotI NruI NsiI NspI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RsrII SacI SacII SalI SbfI ScaI SfcI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspI SwaI TaqI Tru9I Tsp45I TthHB8I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AflIII 727 ACACGT A^C[GA][CT]GT AocI 695 CCTCAGG CC^T[ACGT]AGG AspI 217 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 815 CCCGAG C^[CT]CG[GA]G BbvI 465 GCTGC GCAGC(8/12) BclI 431 TGATCA T^GATCA BglI 199 GCCATGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpmI 67 CTGGAG CTGGAG(16/14) BsiEI 425 CGGTCG CG[GA][CT]^CG BsoBI 815 CCCGAG C^[CT]CG[GA]G Bsp1407I 822 TGTACA T^GTACA BspHI 276 TCATGA T^CATGA BsrFI 204 GCCGGC [GA]^CCGG[CT] BsrGI 822 TGTACA T^GTACA Bsu36I 695 CCTCAGG CC^T[ACGT]AGG Cfr10I 204 GCCGGC [GA]^CCGG[CT] EarI 24 CTCTTC CTCTTC(1/4) Eco81I 695 CCTCAGG CC^T[ACGT]AGG EcoNI 300 CCTCATCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT14I 921 CCAAGG C^C[AT][AT]GG FauI 707 CCCGC CCCGC(4/6) FbaI 431 TGATCA T^GATCA Fnu4HI 465 GCTGC GC^[ACGT]GC HapII 205 CCGG C^CGG HindIII 716 AAGCTT A^AGCTT HpaII 205 CCGG C^CGG ItaI 465 GCTGC GC^[ACGT]GC Ksp632I 24 CTCTTC CTCTTC(1/4) MspI 205 CCGG C^CGG NaeI 204 GCCGGC GCC^GGC NgoMIV 204 GCCGGC G^CCGGC NlaIV 442 GGGACC GG[ACGT]^[ACGT]CC PflFI 217 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 257 CCATATTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 340 GAGTC GAGTC(4/5) RcaI 276 TCATGA T^CATGA SapI 23 GCTCTTC GCTCTTC(1/4) SexAI 218 ACCTGGT A^CC[AT]GGT SfiI 198 GGCCATGCCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SspBI 822 TGTACA T^GTACA StuI 63 AGGCCT AGG^CCT StyI 921 CCAAGG C^C[AT][AT]GG TseI 465 GCTGC G^C[AT]GC Tsp509I 159 AATT ^AATT TspRI 213 CACTG CA[GC]TG[ACGT][ACGT]^ Tth111I 217 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 257 CCATATTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 12 enzymes) # ################################### AciI 708 CCGC CCGC(-3/-1) 795 CCGC AspHI 349 GTGCTC G[AT]GC[AT]^C 547 GAGCAC AvaII 222 GGTCC G^G[AT]CC 443 GGACC BfaI 407 CTAG C^TAG 500 CTAG BsiHKAI 349 GTGCTC G[AT]GC[AT]^C 547 GAGCAC BsmFI 442 GGGAC GGGAC(10/14) 908 GGGAC DpnII 432 GATC ^GATC 903 GATC MaeI 407 CTAG C^TAG 500 CTAG MboI 432 GATC ^GATC 903 GATC NdeII 432 GATC ^GATC 903 GATC Sau3AI 432 GATC ^GATC 903 GATC TfiI 56 GAATC G^A[AT]TC 282 GATTC ######################################### # 3 OR MORE CUT ENZYMES ( 38 enzymes) # ######################################### AfaI AluI BalI BanII BmyI BsaAI BsaJI BseRI BsiYI BslI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI EaeI EcoRII FokI HaeIII HinfI HphI MaeII MboII MluNI MnlI MscI MslI MvaI MwoI NlaIII RsaI Sau96I ScrFI SfaNI XcmI