################################## # NO CUT ENZYMES ( 146 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AvaII AviII AvrII BbeI BbvCI BcgI BciVI BclI BglI BglII BlnI BlpI BpmI Bpu1102I BsaBI BsaHI BsaI BsaWI BseAI BseRI BsiEI BsiWI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspLU11I BspMI BsrFI BsrGI BssHII Bst1107I BstBI BstEII BstPI BstUI BstZ17I Bsu36I CelII Cfr10I Cfr13I ClaI CpoI DraI DraII DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoT22I FbaI FseI FspI HaeII Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MamI MluI MroI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmeI PpuMI PshAI PshBI Psp1406I PstI PvuI RsrII SacI SacII SalI SapI Sau96I SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SspI SwaI Tth111I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AflII 673 CTTAAG C^TTAAG AflIII 694 ACACGT A^C[GA][CT]GT BamHI 9 GGATCC G^GATCC BanI 477 GGCACC G^G[CT][GA]CC BbrPI 695 CACGTG CAC^GTG BcnI 585 CCGGG CC^[GC]GG BfaI 127 CTAG C^TAG BfrI 673 CTTAAG C^TTAAG BsaAI 695 CACGTG [CT]AC^GT[GA] BsgI 467 CTGCAC GTGCAG(16/14) BsmAI 730 GTCTC GTCTC(1/5) BsmBI 729 CGTCTC CGTCTC(1/5) BspHI 560 TCATGA T^CATGA BsrBI 516 CCGCTC CCGCTC(-3/-3) BsrDI 20 GCAATG GCAATG(2/0) BssSI 425 CTCGTG CACGAG(-5/-1) BstAPI 165 GCACAGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstYI 9 GGATCC [GA]^GATC[CT] DpnII 10 GATC ^GATC EarI 70 GAAGAG CTCTTC(1/4) EcoRV 580 GATATC GAT^ATC FauI 459 GCGGG CCCGC(4/6) HapII 585 CCGG C^CGG HgaI 728 GCGTC GACGC(5/10) HpaII 585 CCGG C^CGG Ksp632I 70 GAAGAG CTCTTC(1/4) MaeI 127 CTAG C^TAG MboI 10 GATC ^GATC MfeI 872 CAATTG C^AATTG MflI 9 GGATCC [GA]^GATC[CT] MseI 674 TTAA T^TAA MspI 585 CCGG C^CGG MunI 872 CAATTG C^AATTG NciI 585 CCGGG CC^[GC]GG NdeII 10 GATC ^GATC NspI 200 GCATGC [GA]CATG^[CT] PflMI 257 CCATCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 695 CACGTG CAC^GTG PmlI 695 CACGTG CAC^GTG PvuII 502 CAGCTG CAG^CTG RcaI 560 TCATGA T^CATGA Sau3AI 10 GATC ^GATC ScaI 411 AGTACT AGT^ACT SfcI 304 CTACAG C^T[GA][CT]AG SmlI 673 CTTAAG C^T[CT][GA]AG SphI 200 GCATGC GCATG^C StuI 54 AGGCCT AGG^CCT Tru9I 674 TTAA T^TAA Van91I 257 CCATCTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 9 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### AfaI 412 GTAC GT^AC 763 GTAC AluI 503 AGCT AG^CT 566 AGCT AlwI 10 GATCC GGATC(4/5) 9 GGATC BanII 178 GAGCCC G[GA]GC[CT]^C 949 GAGCCC BbsI 888 GAAGAC GAAGAC(2/6) 490 GTCTTC BpuAI 888 GAAGAC GAAGAC(2/6) 490 GTCTTC BsrI 29 CCAGT ACTGG(1/-1) 285 CCAGT BstXI 245 CCAAAATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 626 CCATTGGCCTGG CfoI 399 GCGC GCG^C 909 GCGC DdeI 77 CTCAG C^T[ACGT]AG 161 CTGAG DsaI 359 CCATGG C^C[GA][CT]GG 782 CCATGG HhaI 399 GCGC GCG^C 909 GCGC HinP1I 399 GCGC G^CGC 909 GCGC HphI 390 TCACC GGTGA(8/7) 431 TCACC MaeII 418 ACGT A^CGT 696 ACGT MslI 550 CACCAAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 736 CATGTGTGTG MspA1I 502 CAGCTG C[AC]G^C[GT]G 817 CCGCTG NcoI 359 CCATGG C^CATGG 782 CCATGG PleI 637 GACTC GAGTC(4/5) 811 GACTC RsaI 412 GTAC GT^AC 763 GTAC TaqI 903 TCGA T^CGA 918 TCGA TthHB8I 903 TCGA T^CGA 918 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 40 enzymes) # ######################################### AciI AspHI BalI BbvI BmyI BsaJI BsiHKAI BsiYI BslI Bsp1286I BssKI BstF5I BstNI Cac8I EaeI EcoRII EcoT14I Fnu4HI FokI HaeIII HinfI ItaI MaeIII MboII MluNI MnlI MscI MvaI MwoI NlaIII NlaIV ScrFI SfaNI StyI TfiI TseI Tsp45I Tsp509I TspRI XcmI