################################## # NO CUT ENZYMES ( 160 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AvaI AviII AvrII BamHI BanI BbeI BbrPI BbvCI BcgI BclI BcnI BfrI BglI BlnI BlpI BpmI Bpu1102I BsaAI BsaHI BsaI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstUI BstXI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HapII HgaI Hin1I HindIII HpaI HpaII KasI KpnI KspI MaeII MfeI MluI MroI MseI MspI MunI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tru9I Tsp509I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AciI 402 CCGC CCGC(-3/-1) AspI 247 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC BbsI 787 GTCTTC GAAGAC(2/6) BciVI 83 GTATCC GTATCC(6/5) BfaI 250 CTAG C^TAG BglII 929 AGATCT A^GATCT BpuAI 787 GTCTTC GAAGAC(2/6) BsaBI 297 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsgI 577 CTGCAC GTGCAG(16/14) BspMI 757 ACCTGC ACCTGC(4/8) BsrDI 425 CATTGC GCAATG(2/0) BsrI 284 CCAGT ACTGG(1/-1) BstEII 160 GGTAACC G^GT[ACGT]ACC BstPI 160 GGTAACC G^GT[ACGT]ACC BstYI 929 AGATCT [GA]^GATC[CT] CfoI 429 GCGC GCG^C DdeI 651 CTCAG C^T[ACGT]AG DraIII 959 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 610 GACACCAGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 725 CCTCTCGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 160 GGTAACC G^GT[ACGT]ACC HhaI 429 GCGC GCG^C HinP1I 429 GCGC G^CGC HincII 895 GTCAAC GT[CT]^[GA]AC HindII 895 GTCAAC GT[CT]^[GA]AC HphI 266 TCACC GGTGA(8/7) MaeI 250 CTAG C^TAG MamI 297 GATGGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MflI 929 AGATCT [GA]^GATC[CT] MspA1I 374 CAGCTG C[AC]G^C[GT]G NcoI 389 CCATGG C^CATGG PflFI 247 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 12 CCAGCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 941 GGGTCCT [GA]G^G[AT]CC[CT] PshAI 168 GACCATTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuII 374 CAGCTG CAG^CTG SfcI 890 CTGTAG C^T[GA][CT]AG TaqI 314 TCGA T^CGA Tsp45I 559 GTCAC ^GT[GC]AC Tth111I 247 GACCTAGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 314 TCGA T^CGA Van91I 12 CCAGCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 929 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### BanII 208 GAGCCC G[GA]GC[CT]^C 808 GGGCTC BmyI 208 GAGCCC G[AGT]GC[ACT]^C 808 GGGCTC BseRI 258 CTCCTC GAGGAG(10/8) 847 CTCCTC BsmFI 22 GTCCC GGGAC(10/14) 836 GTCCC Bsp1286I 208 GAGCCC G[AGT]GC[ACT]^C 808 GGGCTC Cac8I 117 GCTGGC GC[ACGT]^[ACGT]GC 384 GCTGGC DpnII 312 GATC ^GATC 930 GATC DraII 96 GGGGCCT [GA]G^G[ACGT]CC[CT] 941 GGGTCCT DsaI 365 CCGTGG C^C[GA][CT]GG 389 CCATGG EcoO109I 96 GGGGCCT [GA]G^G[ACGT]CC[CT] 941 GGGTCCT EcoT14I 146 CCTTGG C^C[AT][AT]GG 389 CCATGG MaeIII 161 GTAAC ^GT[ACGT]AC 559 GTCAC MboI 312 GATC ^GATC 930 GATC MwoI 355 GCTCTGTCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 739 GCACTCAAGGC NdeII 312 GATC ^GATC 930 GATC Sau3AI 312 GATC ^GATC 930 GATC SfaNI 302 GCATC GCATC(5/9) 761 GCATC SmlI 526 CTCAAG C^T[CT][GA]AG 742 CTCAAG StyI 146 CCTTGG C^C[AT][AT]GG 389 CCATGG TspRI 128 CACTG CA[GC]TG[ACGT][ACGT]^ 512 CACTG XcmI 269 CCATGCCCAAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 614 CCAGGGTCAATGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 34 enzymes) # ######################################### AfaI AluI AvaII BalI BbvI BsaJI BsiYI BslI BssKI BstF5I BstNI Cfr13I EaeI EarI EcoRII Fnu4HI FokI HaeIII HinfI ItaI Ksp632I MboII MluNI MnlI MscI MslI MvaI NlaIII NlaIV PleI RsaI Sau96I ScrFI TseI