(Circular) (Six-base) MAPSORT of: pFN21AE6085.seq Check: 7529 from: 1 to: 5677 pFN21AE6085 5677 bp With 182 enzymes: SgfI * December 8, 2014 13:51 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2771 2771 Size: 5677 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3487 278 Size: 53 83 186 936 1951 2468 Fragments arranged by size: 2468 1951 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3020 1937 Size: 1083 4594 Acc65I G'GTAC_C Cuts at: 2998 2998 Size: 5677 AclI AA'CG_TT Cuts at: 1563 3804 4177 1563 Size: 2241 373 3063 Fragments arranged by size: 3063 2241 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3740 5124 5392 1632 Size: 2108 1384 268 1917 Fragments arranged by size: 2108 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5480 AflIII A'CryG_T Cuts at: 1203 4576 5553 1203 Size: 3373 977 1327 Fragments arranged by size: 3373 1327 977 AgeI A'CCGG_T Cuts at: 4508 4508 Size: 5677 AhdI GACnn_n'nnGTC Cuts at: 4406 4406 Size: 5677 AleI CACnn'nnGTG Cuts at: 1166 2778 1166 Size: 1612 4065 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5677 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2306 2338 2306 Size: 32 5645 AlwNI CAG_nnn'CTG Cuts at: 3420 4992 3420 Size: 1572 4105 ApaLI G'TGCA_C Cuts at: 3734 4890 3734 Size: 1156 4521 ApoI r'AATT_y Cuts at: 1493 2986 3212 3364 5473 1493 Size: 1493 226 152 2109 1697 Fragments arranged by size: 2109 1697 1493 226 152 AseI AT'TA_AT Cuts at: 160 1032 4231 160 Size: 872 3199 1606 Fragments arranged by size: 3199 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5677 AvaI C'yCGr_G Cuts at: 1870 1943 3002 1870 Size: 73 1059 4545 Fragments arranged by size: 4545 1059 73 BamHI G'GATC_C Cuts at: 3007 3374 3007 Size: 367 5310 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2998 4453 618 Size: 325 217 627 1211 1455 1842 Fragments arranged by size: 1842 1455 1211 627 325 217 BanII G_rGCy'C Cuts at: 1391 2217 2817 2996 1391 Size: 826 600 179 4072 Fragments arranged by size: 4072 826 600 179 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2785 928 Size: 971 886 3820 Fragments arranged by size: 3820 971 886 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3868 3902 1903 Size: 34 50 34 1847 34 3678 Fragments arranged by size: 3678 1847 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2099 3569 4779 2099 Size: 1470 1210 2997 Fragments arranged by size: 2997 1470 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 5677 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4288 136 Size: 107 122 71 631 605 2616 1525 Fragments arranged by size: 2616 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2934 5672 2934 Size: 2738 2939 BlpI GC'TnA_GC Cuts at: 3094 3094 Size: 5677 Bme1580I G_kGCm'C Cuts at: 1790 3738 4894 1790 Size: 1948 1156 2573 Fragments arranged by size: 2573 1948 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5677 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3408 4366 442 Size: 416 53 83 399 24 1991 958 1753 Fragments arranged by size: 1991 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5677 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2746 2746 Size: 5677 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4337 1239 Size: 3098 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2516 2732 3802 4667 4965 2516 Size: 216 1070 865 298 3228 Fragments arranged by size: 3228 1070 865 298 216 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4340 882 Size: 617 2841 2219 Fragments arranged by size: 2841 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3468 493 Size: 711 2264 2702 Fragments arranged by size: 2702 2264 711 BsaBI GATnn'nnATC Cuts at: 2306 5671 2306 Size: 3365 2312 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3484 3866 4501 275 Size: 53 83 186 936 1951 382 635 1451 Fragments arranged by size: 1951 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4110 4508 4782 4929 5340 4110 Size: 398 274 147 411 4447 Fragments arranged by size: 4447 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2306 2336 1178 Size: 30 165 30 903 30 4519 Fragments arranged by size: 4519 903 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2254 2843 1186 Size: 1068 589 4020 Fragments arranged by size: 4020 1068 589 BseYI C'CCAG_C Cuts at: 2016 2833 4880 5376 2016 Size: 817 2047 496 2317 Fragments arranged by size: 2317 2047 817 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2567 1163 Size: 573 831 4273 Fragments arranged by size: 4273 831 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3127 3888 4916 1540 Size: 275 186 1126 761 1028 2301 Fragments arranged by size: 2301 1126 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3738 3823 4894 3738 Size: 85 1071 4521 Fragments arranged by size: 4521 1071 85 BsmI GAATG_Cn' Cuts at: 3188 3281 3188 Size: 93 5584 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2217 2817 2996 3738 3823 4894 1391 Size: 399 427 600 179 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 600 427 399 179 85 BspEI T'CCGG_A Cuts at: 5340 5340 Size: 5677 BspHI T'CATG_A Cuts at: 3566 5296 3566 Size: 1730 3947 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2771 3032 844 Size: 1927 261 3489 Fragments arranged by size: 3489 1927 261 BsrBI CCG'CTC Cuts at: 1394 1823 3564 1394 Size: 429 1741 3507 Fragments arranged by size: 3507 1741 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2429 4172 4346 65 Size: 1183 1181 1743 174 1396 Fragments arranged by size: 1743 1396 1183 1181 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4321 4508 1700 Size: 110 2511 187 2869 Fragments arranged by size: 2869 2511 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5677 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5677 BssSI C'ACGA_G Cuts at: 3737 4749 3737 Size: 1012 4665 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5677 BstBI TT'CG_AA Cuts at: 4481 4481 Size: 5677 BstEII G'GTnAC_C Cuts at: 1184 2165 1184 Size: 981 4696 BstYI r'GATC_y Cuts at: 1972 2934 3007 3374 3759 3776 5228 5314 5326 Size: 962 73 367 385 17 1452 86 12 Cuts at: 5326 5672 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 962 385 367 346 86 73 17 12 BtgI C'CryG_G Cuts at: 513 1065 2370 2394 513 Size: 552 1305 24 3796 Fragments arranged by size: 3796 1305 552 24 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4938 Fragments arranged by size: 4938 683 56 BtsI GCAGTG_nn' Cuts at: 2516 3193 3987 4007 2516 Size: 677 794 20 4186 Fragments arranged by size: 4186 794 677 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5677 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5642 DraI TTT'AAA Cuts at: 2981 2981 Size: 5677 DraIII CAC_nnn'GTG Cuts at: 2969 2969 Size: 5677 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2621 4684 809 Size: 507 1305 2063 1802 Fragments arranged by size: 2063 1802 1305 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2154 2232 2286 Size: 54 1425 50 275 18 324 78 54 Cuts at: 2286 2391 2415 2466 3124 4013 5642 8 Size: 105 24 51 658 889 1629 43 Fragments arranged by size: 1629 1425 889 658 324 275 105 78 54 54 51 50 43 24 18 EagI C'GGCC_G Cuts at: 1537 1812 3124 1537 Size: 275 1312 4090 Fragments arranged by size: 4090 1312 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2231 2648 2684 3607 1353 Size: 878 417 36 923 3423 Fragments arranged by size: 3423 923 878 417 36 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4264 4638 4784 387 Size: 1064 138 2675 374 146 1280 Fragments arranged by size: 2675 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3740 4337 5124 5392 1239 Size: 393 2108 597 787 268 1524 Fragments arranged by size: 2108 1524 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2734 2734 Size: 5677 EcoO109I rG'GnC_Cy Cuts at: 2102 2947 2102 Size: 845 4832 EcoRI G'AATT_C Cuts at: 2986 3364 2986 Size: 378 5299 FspI TGC'GCA Cuts at: 4183 4183 Size: 5677 HaeII r_GCGC'y Cuts at: 1678 4824 1678 Size: 3146 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2275 2307 2631 2663 4318 Size: 32 135 32 703 32 324 32 1655 Cuts at: 4318 4350 4392 4424 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1655 703 324 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2902 3021 3267 669 Size: 988 281 964 119 246 3079 Fragments arranged by size: 3079 988 964 281 246 119 HindIII A'AGCT_T Cuts at: 748 748 Size: 5677 HpaI GTT'AAC Cuts at: 3267 3267 Size: 5677 KpnI G_GTAC'C Cuts at: 3002 3002 Size: 5677 MfeI C'AATT_G Cuts at: 3276 3276 Size: 5677 MluI A'CGCG_T Cuts at: 5553 5553 Size: 5677 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4791 4975 1305 Size: 3486 184 2007 Fragments arranged by size: 3486 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2156 2234 2288 2393 2417 Size: 54 1425 343 324 78 54 105 24 Cuts at: 2417 2468 10 Size: 51 3219 Fragments arranged by size: 3219 1425 343 324 105 78 54 54 51 24 MslI CAynn'nnrTG Cuts at: 518 1166 2192 2666 2778 3635 3994 4153 518 Size: 648 1026 474 112 857 359 159 2042 Fragments arranged by size: 2042 1026 857 648 474 359 159 112 MspA1I CmG'CkG Cuts at: 1606 2381 3093 3770 4918 5163 1606 Size: 775 712 677 1148 245 2120 Fragments arranged by size: 2120 1148 775 712 677 245 NcoI C'CATG_G Cuts at: 513 1065 2394 513 Size: 552 1329 3796 Fragments arranged by size: 3796 1329 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5677 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5677 NotI GC'GGCC_GC Cuts at: 3124 3124 Size: 5677 NspI r_CATG'y Cuts at: 1132 1229 3035 4580 1132 Size: 97 1806 1545 2229 Fragments arranged by size: 2229 1806 1545 97 PasI CC'CwG_GG Cuts at: 1348 2161 1348 Size: 813 4864 PciI A'CATG_T Cuts at: 4576 4576 Size: 5677 PflMI CCAn_nnn'nTGG Cuts at: 1108 2023 1108 Size: 915 4762 PfoI T'CCnGG_A Cuts at: 2310 2310 Size: 5677 PmeI GTTT'AAAC Cuts at: 2981 2981 Size: 5677 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2306 2338 3746 3778 5259 5291 2306 Size: 32 1408 32 1481 32 2692 Fragments arranged by size: 2692 1481 1408 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2947 2947 Size: 5677 PshAI GACnn'nnGTC Cuts at: 1613 2177 1613 Size: 564 5113 PsiI TTA'TAA Cuts at: 3247 3247 Size: 5677 PstI C_TGCA'G Cuts at: 830 3029 830 Size: 2199 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5677 PvuII CAG'CTG Cuts at: 2381 2381 Size: 5677 SalI G'TCGA_C Cuts at: 1936 3019 1936 Size: 1083 4594 SbfI CC_TGCA'GG Cuts at: 3029 3029 Size: 5677 ScaI AGT'ACT Cuts at: 3925 5637 3925 Size: 1712 3965 SfcI C'TryA_G Cuts at: 826 1045 2895 3025 4160 4841 5032 826 Size: 219 1850 130 1135 681 191 1471 Fragments arranged by size: 1850 1471 1135 681 219 191 130 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5677 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5677 SmaI CCC'GGG Cuts at: 1872 3004 1872 Size: 1132 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2531 2747 3781 4682 4944 820 Size: 197 926 588 216 1034 901 262 1553 Fragments arranged by size: 1553 1034 926 901 588 262 216 197 SnaBI TAC'GTA Cuts at: 493 3468 493 Size: 2975 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5677 SphI G_CATG'C Cuts at: 1132 3035 1132 Size: 1903 3774 SspI AAT'ATT Cuts at: 5 52 3601 5 Size: 47 3549 2081 Fragments arranged by size: 3549 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5677 StyI C'CwwG_G Cuts at: 513 1065 2151 2394 3116 513 Size: 552 1086 243 722 3074 Fragments arranged by size: 3074 1086 722 552 243 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2442 2654 2825 3704 3721 3874 4059 Size: 1255 862 212 171 879 17 153 185 Cuts at: 4059 4504 325 Size: 445 1498 Fragments arranged by size: 1498 1255 879 862 445 212 185 171 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2459 2609 3923 Size: 274 80 33 51 1443 482 150 1314 Cuts at: 3923 5635 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1314 482 274 150 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5637 5669 5637 Size: 32 5645 Tth111I GACn'n_nGTC Cuts at: 1316 2255 1316 Size: 939 4738 XbaI T'CTAG_A Cuts at: 3013 3013 Size: 5677 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2281 2626 2281 Size: 345 5332 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5677 XmaI C'CCGG_G Cuts at: 1870 3002 1870 Size: 1132 4545 XmnI GAAnn'nnTTC Cuts at: 3806 3806 Size: 5677 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3485 276 Size: 53 83 186 936 1951 2468 Fragments arranged by size: 2468 1951 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI