################################## # NO CUT ENZYMES ( 159 enzymes) # ################################## AatII AccI AccII AccIII AclI AcyI AflII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I AspEI AspI AvaI AviII AvrII BamHI BbeI BbrPI BbsI BbvCI BcgI BcnI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaBI BsaHI BsaWI BseAI BsgI BsiEI BsiWI BsmFI BsoBI BspDI BspEI BspHI BspMI BsrBI BsrFI BsrI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstYI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraII DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRV FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HindIII HpaI HpaII KasI Ksp632I KspI MamI MfeI MflI MluI MroI MspI MunI MvnI NaeI NarI NciI NdeI NgoMIV NotI NruI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI StuI SwaI TaqI Tth111I TthHB8I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### Acc65I 321 GGTACC G^GTACC AcsI 491 GAATTC [GA]^AATT[CT] AflIII 808 ACATGT A^C[GA][CT]GT ApoI 491 GAATTC [GA]^AATT[CT] Asp718I 321 GGTACC G^GTACC AspHI 628 GAGCAC G[AT]GC[AT]^C BanI 321 GGTACC G^G[CT][GA]CC BciVI 137 GTATCC GTATCC(6/5) BclI 935 TGATCA T^GATCA BfaI 919 CTAG C^TAG BsaAI 931 TACGTG [CT]AC^GT[GA] BsaI 769 GGTCTC GGTCTC(1/5) BsiHKAI 628 GAGCAC G[AT]GC[AT]^C BsmBI 421 GAGACG CGTCTC(1/5) BsmI 26 GCATTC GAATGC(1/-1) Bsp1407I 191 TGTACA T^GTACA BspLU11I 808 ACATGT A^CATGT BsrDI 729 CATTGC GCAATG(2/0) BsrGI 191 TGTACA T^GTACA BstAPI 338 GCATCTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC DpnII 936 GATC ^GATC DraIII 179 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 440 CCATGG C^C[GA][CT]GG EcoRI 491 GAATTC G^AATTC EcoT14I 440 CCATGG C^C[AT][AT]GG EcoT22I 1015 ATGCAT ATGCA^T FauI 990 GCGGG CCCGC(4/6) FbaI 935 TGATCA T^GATCA HincII 5 GTCAAC GT[CT]^[GA]AC HindII 5 GTCAAC GT[CT]^[GA]AC KpnI 321 GGTACC GGTAC^C MaeI 919 CTAG C^TAG MaeII 932 ACGT A^CGT MaeIII 226 GTGAC ^GT[ACGT]AC MboI 936 GATC ^GATC MspA1I 413 CAGCTG C[AC]G^C[GT]G NcoI 440 CCATGG C^CATGG NdeII 936 GATC ^GATC NheI 918 GCTAGC G^CTAGC NsiI 1015 ATGCAT ATGCA^T PflMI 99 CCACACAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 579 GAGTC GAGTC(4/5) PvuII 413 CAGCTG CAG^CTG Sau3AI 936 GATC ^GATC SfcI 681 CTACAG C^T[GA][CT]AG SspBI 191 TGTACA T^GTACA StyI 440 CCATGG C^C[AT][AT]GG TfiI 244 GATTC G^A[AT]TC Tsp45I 226 GTGAC ^GT[GC]AC Van91I 99 CCACACAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 14 enzymes) # ################################### AvaII 85 GGTCC G^G[AT]CC 977 GGACC BalI 527 TGGCCA TGG^CCA 641 TGGCCA BanII 259 GAGCCC G[GA]GC[CT]^C 711 GGGCTC BseRI 312 CTCCTC GAGGAG(10/8) 774 CTCCTC BstXI 410 CCACAGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 562 CCACTGAGTTGG Cfr13I 85 GGTCC G^G[ACGT]CC 977 GGACC EaeI 527 TGGCCA [CT]^GGCC[GA] 641 TGGCCA HinfI 244 GATTC G^A[ACGT]TC 579 GAGTC MluNI 527 TGGCCA TGG^CCA 641 TGGCCA MscI 527 TGGCCA TGG^CCA 641 TGGCCA Sau96I 85 GGTCC G^G[ACGT]CC 977 GGACC SmlI 503 CTCAAG C^T[CT][GA]AG 754 CTCAAG Tsp509I 492 AATT ^AATT 750 AATT TspRI 179 CACTG CA[GC]TG[ACGT][ACGT]^ 563 CACTG ######################################### # 3 OR MORE CUT ENZYMES ( 35 enzymes) # ######################################### AciI AfaI AluI BbvI BmyI BsaJI BsiYI BslI BsmAI Bsp1286I BssKI BstF5I BstNI Cac8I DdeI EcoRII Fnu4HI FokI HaeIII HphI ItaI MboII MnlI MseI MslI MvaI MwoI NlaIII NlaIV NspI RsaI ScrFI SfaNI Tru9I TseI