(Circular) (Six-base) MAPSORT of: pFN21AE5496.seq Check: 3523 from: 1 to: 5755 pFN21AE5496 5755 bp With 182 enzymes: SgfI * January 30, 2014 17:21 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3565 278 Size: 53 83 186 936 2029 2468 Fragments arranged by size: 2468 2029 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3098 1937 Size: 1161 4594 Acc65I G'GTAC_C Cuts at: 2326 3076 2326 Size: 750 5005 AclI AA'CG_TT Cuts at: 1563 3882 4255 1563 Size: 2319 373 3063 Fragments arranged by size: 3063 2319 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3818 5202 5470 1632 Size: 2186 1384 268 1917 Fragments arranged by size: 2186 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5558 AflIII A'CryG_T Cuts at: 1203 2813 4654 5631 1203 Size: 1610 1841 977 1327 Fragments arranged by size: 1841 1610 1327 977 AgeI A'CCGG_T Cuts at: 4586 4586 Size: 5755 AhdI GACnn_n'nnGTC Cuts at: 4484 4484 Size: 5755 AleI CACnn'nnGTG Cuts at: 1166 2420 1166 Size: 1254 4501 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5755 AlwNI CAG_nnn'CTG Cuts at: 3498 5070 3498 Size: 1572 4183 ApaLI G'TGCA_C Cuts at: 3812 4968 3812 Size: 1156 4599 ApoI r'AATT_y Cuts at: 1493 2496 3064 3290 3442 5551 1493 Size: 1003 568 226 152 2109 1697 Fragments arranged by size: 2109 1697 1003 568 226 152 AseI AT'TA_AT Cuts at: 160 1032 4309 160 Size: 872 3277 1606 Fragments arranged by size: 3277 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5755 AvaI C'yCGr_G Cuts at: 1870 1943 3080 1870 Size: 73 1137 4545 Fragments arranged by size: 4545 1137 73 BamHI G'GATC_C Cuts at: 3085 3452 3085 Size: 367 5388 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2326 3076 4531 618 Size: 325 217 627 539 750 1455 1842 Fragments arranged by size: 1842 1455 750 627 539 325 217 BanII G_rGCy'C Cuts at: 1391 2268 2720 3074 1391 Size: 877 452 354 4072 Fragments arranged by size: 4072 877 452 354 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4784 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3946 3980 1903 Size: 34 2009 34 3678 Fragments arranged by size: 3678 2009 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2153 3647 4857 2153 Size: 1494 1210 3051 Fragments arranged by size: 3051 1494 1210 BclI T'GATC_A Cuts at: 1548 2940 1548 Size: 1392 4363 BfrBI ATG'CAT Cuts at: 3022 3022 Size: 5755 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4366 136 Size: 107 122 71 631 605 2694 1525 Fragments arranged by size: 2694 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5750 5750 Size: 5755 BlpI GC'TnA_GC Cuts at: 3172 3172 Size: 5755 Bme1580I G_kGCm'C Cuts at: 1790 2676 2904 3816 4972 1790 Size: 886 228 912 1156 2573 Fragments arranged by size: 2573 1156 912 886 228 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5755 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3486 4444 442 Size: 416 53 83 399 24 2069 958 1753 Fragments arranged by size: 2069 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2927 1056 Size: 1871 3884 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4415 1239 Size: 3176 2579 Bpu10I CC'TnA_GC Cuts at: 2353 2353 Size: 5755 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2493 2744 3880 4745 5043 2493 Size: 251 1136 865 298 3205 Fragments arranged by size: 3205 1136 865 298 251 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2780 4418 882 Size: 617 1281 1638 2219 Fragments arranged by size: 2219 1638 1281 617 BsaAI yAC'GTr Cuts at: 493 1204 2938 3546 493 Size: 711 1734 608 2702 Fragments arranged by size: 2702 1734 711 608 BsaBI GATnn'nnATC Cuts at: 5749 5749 Size: 5755 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3562 3944 4579 275 Size: 53 83 186 936 2029 382 635 1451 Fragments arranged by size: 2029 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4188 4586 4860 5007 5418 4188 Size: 398 274 147 411 4525 Fragments arranged by size: 4525 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5530 Fragments arranged by size: 5530 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2308 2770 1186 Size: 1122 462 4171 Fragments arranged by size: 4171 1122 462 BseYI C'CCAG_C Cuts at: 2086 2518 2675 2947 4958 5454 2086 Size: 432 157 272 2011 496 2387 Fragments arranged by size: 2387 2011 496 432 272 157 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5182 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3205 3966 4994 1540 Size: 275 186 1204 761 1028 2301 Fragments arranged by size: 2301 1204 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2637 3816 3901 4972 2637 Size: 1179 85 1071 3420 Fragments arranged by size: 3420 1179 1071 85 BsmI GAATG_Cn' Cuts at: 2031 3266 3359 2031 Size: 1235 93 4427 Fragments arranged by size: 4427 1235 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2420 2420 Size: 5755 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2268 2637 2676 2720 2904 3074 3816 Size: 399 478 369 39 44 184 170 742 Cuts at: 3816 3901 4972 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 478 399 369 184 170 85 44 39 BspEI T'CCGG_A Cuts at: 5418 5418 Size: 5755 BspHI T'CATG_A Cuts at: 3644 5374 3644 Size: 1730 4025 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3110 844 Size: 2266 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3642 1394 Size: 429 1819 3507 Fragments arranged by size: 3507 1819 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2733 4250 4424 65 Size: 1183 1485 1517 174 1396 Fragments arranged by size: 1517 1485 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4399 4586 1700 Size: 110 2589 187 2869 Fragments arranged by size: 2869 2589 187 110 BsrGI T'GTAC_A Cuts at: 96 2196 96 Size: 2100 3655 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5755 BssSI C'ACGA_G Cuts at: 3815 4827 3815 Size: 1012 4743 BstAPI GCAn_nnn'nTGC Cuts at: 794 2349 794 Size: 1555 4200 BstBI TT'CG_AA Cuts at: 4559 4559 Size: 5755 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5755 BstXI CCAn_nnnn'nTGG Cuts at: 2422 2574 2422 Size: 152 5603 BstYI r'GATC_y Cuts at: 1972 3085 3452 3837 3854 5306 5392 5404 5750 Size: 1113 367 385 17 1452 86 12 346 Cuts at: 5750 1972 Size: 1977 Fragments arranged by size: 1977 1452 1113 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2445 513 Size: 552 1380 3823 Fragments arranged by size: 3823 1380 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5016 Fragments arranged by size: 5016 683 56 BtsI GCAGTG_nn' Cuts at: 3271 4065 4085 3271 Size: 794 20 4941 Fragments arranged by size: 4941 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5755 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5720 DraI TTT'AAA Cuts at: 3059 3059 Size: 5755 DraIII CAC_nnn'GTG Cuts at: 2189 2189 Size: 5755 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4762 809 Size: 507 3446 1802 Fragments arranged by size: 3446 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2532 2646 3202 Size: 54 1425 50 275 18 702 114 556 Cuts at: 3202 4091 5720 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 702 556 275 114 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3202 1537 Size: 275 1390 4090 Fragments arranged by size: 4090 1390 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3685 1353 Size: 2332 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4342 4716 4862 387 Size: 1064 138 2753 374 146 1280 Fragments arranged by size: 2753 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3818 4415 5202 5470 1239 Size: 393 2186 597 787 268 1524 Fragments arranged by size: 2186 1524 787 597 393 268 EcoRI G'AATT_C Cuts at: 2496 3064 3442 2496 Size: 568 378 4809 Fragments arranged by size: 4809 568 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2093 2093 Size: 5755 FspI TGC'GCA Cuts at: 4261 4261 Size: 5755 HaeII r_GCGC'y Cuts at: 1678 4902 1678 Size: 3224 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2074 2106 2569 2601 4396 Size: 32 135 32 502 32 463 32 1795 Cuts at: 4396 4428 4470 4502 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1795 502 463 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2012 3099 3345 669 Size: 988 281 74 1087 246 3079 Fragments arranged by size: 3079 1087 988 281 246 74 HindIII A'AGCT_T Cuts at: 748 748 Size: 5755 HpaI GTT'AAC Cuts at: 3345 3345 Size: 5755 KpnI G_GTAC'C Cuts at: 2330 3080 2330 Size: 750 5005 MfeI C'AATT_G Cuts at: 3354 3354 Size: 5755 MluI A'CGCG_T Cuts at: 5631 5631 Size: 5755 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2555 3066 4869 5053 1305 Size: 1250 511 1803 184 2007 Fragments arranged by size: 2007 1803 1250 511 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2534 2648 10 Size: 54 1425 343 702 114 3117 Fragments arranged by size: 3117 1425 702 343 114 54 MslI CAynn'nnrTG Cuts at: 518 1166 2309 2393 2420 3713 4072 4231 518 Size: 648 1143 84 27 1293 359 159 2042 Fragments arranged by size: 2042 1293 1143 648 359 159 84 27 MspA1I CmG'CkG Cuts at: 1606 2420 3171 3848 4996 5241 1606 Size: 814 751 677 1148 245 2120 Fragments arranged by size: 2120 1148 814 751 677 245 NcoI C'CATG_G Cuts at: 513 1065 2445 513 Size: 552 1380 3823 Fragments arranged by size: 3823 1380 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5755 NheI G'CTAG_C Cuts at: 1052 2923 1052 Size: 1871 3884 NotI GC'GGCC_GC Cuts at: 3202 3202 Size: 5755 NsiI A_TGCA'T Cuts at: 3024 3024 Size: 5755 NspI r_CATG'y Cuts at: 1132 1229 2661 2817 3026 3113 4658 1132 Size: 97 1432 156 209 87 1545 2229 Fragments arranged by size: 2229 1545 1432 209 156 97 87 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5755 PciI A'CATG_T Cuts at: 2813 4654 2813 Size: 1841 3914 PflMI CCAn_nnn'nTGG Cuts at: 1108 2110 1108 Size: 1002 4753 PmeI GTTT'AAAC Cuts at: 3059 3059 Size: 5755 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3824 3856 5337 5369 3824 Size: 32 1481 32 4210 Fragments arranged by size: 4210 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5755 PsiI TTA'TAA Cuts at: 2100 3325 2100 Size: 1225 4530 PstI C_TGCA'G Cuts at: 830 3107 830 Size: 2277 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5755 PvuII CAG'CTG Cuts at: 2420 2420 Size: 5755 SalI G'TCGA_C Cuts at: 1936 3097 1936 Size: 1161 4594 SbfI CC_TGCA'GG Cuts at: 3107 3107 Size: 5755 ScaI AGT'ACT Cuts at: 4003 5715 4003 Size: 1712 4043 SfcI C'TryA_G Cuts at: 826 1045 2686 3103 4238 4919 5110 826 Size: 219 1641 417 1135 681 191 1471 Fragments arranged by size: 1641 1471 1135 681 417 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5755 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5755 SmaI CCC'GGG Cuts at: 1872 3082 1872 Size: 1210 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2508 2759 3859 4760 5022 820 Size: 197 926 565 251 1100 901 262 1553 Fragments arranged by size: 1553 1100 926 901 565 262 251 197 SnaBI TAC'GTA Cuts at: 493 3546 493 Size: 3053 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5755 SphI G_CATG'C Cuts at: 1132 3113 1132 Size: 1981 3774 SspI AAT'ATT Cuts at: 5 52 3679 5 Size: 47 3627 2081 Fragments arranged by size: 3627 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5755 StyI C'CwwG_G Cuts at: 513 1065 2445 3194 513 Size: 552 1380 749 3074 Fragments arranged by size: 3074 1380 749 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2927 3782 3799 3952 4137 4582 325 Size: 1255 1347 855 17 153 185 445 1498 Fragments arranged by size: 1498 1347 1255 855 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2196 2844 4001 Size: 274 80 33 51 1443 219 648 1157 Cuts at: 4001 5713 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1157 648 274 219 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2944 2976 5715 5747 2944 Size: 32 2739 32 2952 Fragments arranged by size: 2952 2739 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5755 XbaI T'CTAG_A Cuts at: 3091 3091 Size: 5755 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5755 XmaI C'CCGG_G Cuts at: 1870 3080 1870 Size: 1210 4545 XmnI GAAnn'nnTTC Cuts at: 3884 3884 Size: 5755 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3563 276 Size: 53 83 186 936 2029 2468 Fragments arranged by size: 2468 2029 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI BsiWI BstZ17I Bsu36I EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI