(Linear) (Six-base) MAPSORT of: KIEE5496.seq Check: 520 from: 1 to: 1050 KIEE5496.orf With 182 enzymes: SgfI * January 29, 2014 12:03 .. Acc65I G'GTAC_C Cuts at: 0 321 1050 Size: 321 729 AflIII A'CryG_T Cuts at: 0 808 1050 Size: 808 242 AleI CACnn'nnGTG Cuts at: 0 415 1050 Size: 415 635 ApoI r'AATT_y Cuts at: 0 491 1050 Size: 491 559 BanI G'GyrC_C Cuts at: 0 321 1050 Size: 321 729 BanII G_rGCy'C Cuts at: 0 263 715 1050 Size: 263 452 335 Fragments arranged by size: 452 335 263 BciVI GTATCCnnnnn_n' Cuts at: 0 148 1050 Size: 148 902 BclI T'GATC_A Cuts at: 0 935 1050 Size: 935 115 BfrBI ATG'CAT Cuts at: 0 1017 1050 Size: 1017 33 Bme1580I G_kGCm'C Cuts at: 0 671 899 1050 Size: 671 228 151 Fragments arranged by size: 671 228 151 BmtI G_CTAG'C Cuts at: 0 922 1050 Size: 922 128 Bpu10I CC'TnA_GC Cuts at: 0 348 1050 Size: 348 702 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 488 739 1050 Size: 488 251 311 Fragments arranged by size: 488 311 251 BsaI GGTCTCn'nnnn_ Cuts at: 0 775 1050 Size: 775 275 BsaAI yAC'GTr Cuts at: 0 933 1050 Size: 933 117 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 303 765 1050 Size: 303 462 285 Fragments arranged by size: 462 303 285 BseYI C'CCAG_C Cuts at: 0 81 513 670 942 1050 Size: 81 432 157 272 108 Fragments arranged by size: 432 272 157 108 81 BsiHKAI G_wGCw'C Cuts at: 0 632 1050 Size: 632 418 BsmI GAATG_Cn' Cuts at: 0 26 1050 Size: 26 1024 BsmBI CGTCTCn'nnnn_ Cuts at: 0 415 1050 Size: 415 635 Bsp1286I G_dGCh'C Cuts at: 0 263 632 671 715 899 1050 Size: 263 369 39 44 184 151 Fragments arranged by size: 369 263 184 151 44 39 BsrDI GCAATG_nn' Cuts at: 0 728 1050 Size: 728 322 BsrGI T'GTAC_A Cuts at: 0 191 1050 Size: 191 859 BstAPI GCAn_nnn'nTGC Cuts at: 0 344 1050 Size: 344 706 BstXI CCAn_nnnn'nTGG Cuts at: 0 417 569 1050 Size: 417 152 481 Fragments arranged by size: 481 417 152 BtgI C'CryG_G Cuts at: 0 440 1050 Size: 440 610 DraIII CAC_nnn'GTG Cuts at: 0 184 1050 Size: 184 866 EaeI y'GGCC_r Cuts at: 0 527 641 1050 Size: 527 114 409 Fragments arranged by size: 527 409 114 EcoRI G'AATT_C Cuts at: 0 491 1050 Size: 491 559 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 88 1050 Size: 88 962 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 69 101 564 596 1050 Size: 69 32 463 32 454 Fragments arranged by size: 463 454 69 32 32 HincII GTy'rAC Cuts at: 0 7 1050 Size: 7 1043 KpnI G_GTAC'C Cuts at: 0 325 1050 Size: 325 725 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 550 1050 Size: 550 500 MscI TGG'CCA Cuts at: 0 529 643 1050 Size: 529 114 407 Fragments arranged by size: 529 407 114 MslI CAynn'nnrTG Cuts at: 0 304 388 415 1050 Size: 304 84 27 635 Fragments arranged by size: 635 304 84 27 MspA1I CmG'CkG Cuts at: 0 415 1050 Size: 415 635 NcoI C'CATG_G Cuts at: 0 440 1050 Size: 440 610 NheI G'CTAG_C Cuts at: 0 918 1050 Size: 918 132 NsiI A_TGCA'T Cuts at: 0 1019 1050 Size: 1019 31 NspI r_CATG'y Cuts at: 0 656 812 1021 1050 Size: 656 156 209 29 Fragments arranged by size: 656 209 156 29 PciI A'CATG_T Cuts at: 0 808 1050 Size: 808 242 PflMI CCAn_nnn'nTGG Cuts at: 0 105 1050 Size: 105 945 PsiI TTA'TAA Cuts at: 0 95 1050 Size: 95 955 PvuII CAG'CTG Cuts at: 0 415 1050 Size: 415 635 SfcI C'TryA_G Cuts at: 0 681 1050 Size: 681 369 SmlI C'TyrA_G Cuts at: 0 503 754 1050 Size: 503 251 296 Fragments arranged by size: 503 296 251 StyI C'CwwG_G Cuts at: 0 440 1050 Size: 440 610 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 922 1050 Size: 922 128 TatI w'GTAC_w Cuts at: 0 191 839 1050 Size: 191 648 211 Fragments arranged by size: 648 211 191 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 939 971 1050 Size: 939 32 79 Fragments arranged by size: 939 79 32 Enzymes that do cut: Acc65I AflIII AleI ApoI BanI BanII BciVI BclI BfrBI Bme1580I BmtI Bpu10I BpuEI BsaI BsaAI BseRI BseYI BsiHKAI BsmI BsmBI Bsp1286I BsrDI BsrGI BstAPI BstXI BtgI DraIII EaeI EcoRI FalI Hin4I HincII KpnI MmeI MscI MslI MspA1I NcoI NheI NsiI NspI PciI PflMI PsiI PvuII SfcI SmlI StyI TaqII TatI TstI Enzymes that do not cut: AarI AatII AccI AclI AcuI AfeI AflII AgeI AhdI AlfI AloI AlwNI ApaI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BamHI BbeI BbsI BbvCI BcgI BglI BglII BlpI BmgBI BmrI BplI BpmI BsaBI BsaHI BsaWI BsaXI BsgI BsiEI BsiWI BspEI BspHI BspMI BsrBI BsrFI BssHII BssSI BstBI BstEII BstYI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI DrdI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRV FseI FspI FspAI HaeII HindIII HpaI KasI MfeI MluI NaeI NarI NdeI NgoMIV NotI NruI PacI PasI PfoI PmeI PmlI PpiI PpuMI PshAI PspOMI PspXI PsrI PstI PvuI RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI