################################## # NO CUT ENZYMES ( 73 enzymes) # ################################## Acc65I AccI AccIII AclI AflII AgeI AhdI Alw44I ApaLI AscI AseI AsnI Asp718I AspEI AvrII BciVI BclI BfrI BlnI BsaWI BseAI BsiWI Bsp1407I BspDI BspEI BsrGI BssHII Bst1107I BstBI BstZ17I ClaI CpoI DraI Eam1105I EcoRI EcoRV EcoT22I FbaI HindIII HpaI KpnI MfeI MluI MroI MunI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmeI PshAI PshBI Psp1406I PvuI RsrII SalI ScaI SfuI SgrAI SnaBI SpeI SphI SspBI SspI SwaI TfiI XbaI XhoI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AatII 2614 GACGTC GACGT^C AcsI 2176 AAATTC [GA]^AATT[CT] Aor51HI 2987 AGCGCT AGC^GCT ApoI 2176 AAATTC [GA]^AATT[CT] Asp700I 2388 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 979 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 263 TGCGCA TGC^GCA BamHI 305 GGATCC G^GATCC BbeI 2115 GGCGCC GGCGC^C BbrPI 2144 CACGTG CAC^GTG BbvCI 603 GCTGAGG CCTCAGC(-5/-2) BsaAI 2144 CACGTG [CT]AC^GT[GA] BsaBI 2547 GATCCATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsmBI 1684 GAGACG CGTCTC(1/5) BsmI 446 GCATTC GAATGC(1/-1) BspHI 2909 TCATGA T^CATGA BspLU11I 2969 ACATGT A^CATGT BsrDI 3261 CATTGC GCAATG(2/0) BstAPI 351 GCAGGCCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Eco47III 2987 AGCGCT AGC^GCT FspI 263 TGCGCA TGC^GCA HincII 82 GTCAAC GT[CT]^[GA]AC HindII 82 GTCAAC GT[CT]^[GA]AC KasI 2115 GGCGCC G^GCGCC KspI 1364 CCGCGG CCGC^GG MamI 2547 GATCCATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MseI 845 TTAA T^TAA NarI 2115 GGCGCC GG^CGCC PflFI 979 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 2144 CACGTG CAC^GTG PmlI 2144 CACGTG CAC^GTG RcaI 2909 TCATGA T^CATGA SacII 1364 CCGCGG CCGC^GG SfiI 1692 GGCCGAGGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 2115 GGCGCC GGC^GCC Tru9I 845 TTAA T^TAA Tsp509I 2177 AATT ^AATT Tth111I 979 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC XmnI 2388 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AflIII 2939 ACACGT A^C[GA][CT]GT 2969 ACATGT BcgI 780 CGATGTGCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 2504 GCACAGTCCTCG BfaI 796 CTAG C^TAG 1951 CTAG BglII 1100 AGATCT A^GATCT 2747 AGATCT BlpI 3048 GCTCAGC GC^T[ACGT]AGC 3094 GCTGAGC Bpu1102I 3048 GCTCAGC GC^T[ACGT]AGC 3094 GCTGAGC BsaI 3122 GAGACC GGTCTC(1/5) 1395 GGTCTC BsiEI 1452 CGGCCG CG[GA][CT]^CG 2697 CGGCCG CelII 3048 GCTCAGC GC^T[ACGT]AGC 3094 GCTGAGC DraIII 2380 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG 3247 CACAGAGTG DrdI 1156 GACGCCCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 3142 GACCAGAAGGTC EagI 1452 CGGCCG C^GGCCG 2697 CGGCCG EclXI 1452 CGGCCG C^GGCCG 2697 CGGCCG Eco52I 1452 CGGCCG C^GGCCG 2697 CGGCCG FseI 1329 GGCCGGCC GGCCGG^CC 1453 GGCCGGCC HinfI 2824 GACTC G^A[ACGT]TC 3316 GAGTC MaeI 796 CTAG C^TAG 1951 CTAG NcoI 1616 CCATGG C^CATGG 2516 CCATGG NspI 342 GCATGT [GA]CATG^[CT] 2969 ACATGT PleI 2824 GACTC GAGTC(4/5) 3316 GAGTC PvuII 2572 CAGCTG CAG^CTG 3418 CAGCTG SexAI 197 ACCTGGT A^CC[AT]GGT 2381 ACCTGGT SmlI 1054 CTCAAG C^T[CT][GA]AG 1408 CTCAAG ######################################### # 3 OR MORE CUT ENZYMES ( 123 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI AlwNI AocI ApaI AspHI AvaI AvaII BalI BanI BanII BbsI BbvI BcnI BglI BmyI BpmI BpuAI BsaHI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrFI BsrI BssKI BssSI BstEII BstF5I BstNI BstPI BstUI BstXI BstYI Bsu36I Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoO65I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HpaII HphI ItaI Ksp632I MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MslI MspA1I MspI MvaI MvnI MwoI NaeI NciI NdeII NgoMIV NlaIII NlaIV PflMI PpuMI PstI RsaI SacI SapI Sau3AI Sau96I SbfI ScrFI SfaNI SfcI SmaI Sse8387I StuI StyI TaqI TseI Tsp45I TspRI TthHB8I Van91I XcmI XhoII XmaCI XmaI