(Linear) (Six-base) MAPSORT of: KIEE2791.seq Check: 447 from: 1 to: 3495 KIEE2791.orf With 182 enzymes: SgfI * February 19, 2021 20:23 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 191 941 1837 2476 3495 Size: 191 750 896 639 1019 Fragments arranged by size: 1019 896 750 639 191 AatII G_ACGT'C Cuts at: 0 2618 3495 Size: 2618 877 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 65 1012 1983 2253 3495 Size: 65 947 971 270 1242 Fragments arranged by size: 1242 971 947 270 65 AfeI AGC'GCT Cuts at: 0 2989 3495 Size: 2989 506 AflIII A'CryG_T Cuts at: 0 2939 2969 3495 Size: 2939 30 526 Fragments arranged by size: 2939 526 30 AleI CACnn'nnGTG Cuts at: 0 2899 3440 3495 Size: 2899 541 55 Fragments arranged by size: 2899 541 55 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1294 2494 3495 Size: 1294 1200 1001 Fragments arranged by size: 1294 1200 1001 AlwNI CAG_nnn'CTG Cuts at: 0 933 1907 2046 2679 3495 Size: 933 974 139 633 816 Fragments arranged by size: 974 933 816 633 139 ApaI G_GGCC'C Cuts at: 0 514 1489 2290 2300 2581 3495 Size: 514 975 801 10 281 914 Fragments arranged by size: 975 914 801 514 281 10 ApoI r'AATT_y Cuts at: 0 2176 3495 Size: 2176 1319 AvaI C'yCGr_G Cuts at: 0 34 570 765 1177 1352 1703 2299 3274 Size: 34 536 195 412 175 351 596 975 Cuts at: 3274 3466 3495 Size: 192 29 Fragments arranged by size: 975 596 536 412 351 195 192 175 34 29 BamHI G'GATC_C Cuts at: 0 305 3495 Size: 305 3190 BanI G'GyrC_C Cuts at: 0 126 179 819 1339 2115 3495 Size: 126 53 640 520 776 1380 Fragments arranged by size: 1380 776 640 520 126 53 BanII G_rGCy'C Cuts at: 0 514 967 1055 1253 1489 2074 2127 2290 Size: 514 453 88 198 236 585 53 163 Cuts at: 2290 2300 2581 3394 3495 Size: 10 281 813 101 Fragments arranged by size: 813 585 514 453 281 236 198 163 101 88 53 10 BbeI G_GCGC'C Cuts at: 0 2119 3495 Size: 2119 1376 BbsI GAAGACnn'nnnn_ Cuts at: 0 53 2145 2610 3072 3495 Size: 53 2092 465 462 423 Fragments arranged by size: 2092 465 462 423 53 BbvCI CC'TCA_GC Cuts at: 0 604 3495 Size: 604 2891 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 769 803 2493 2527 3495 Size: 769 34 1690 34 968 Fragments arranged by size: 1690 968 769 34 34 BglI GCCn_nnn'nGGC Cuts at: 0 1645 1699 1849 3495 Size: 1645 54 150 1646 Fragments arranged by size: 1646 1645 150 54 BglII A'GATC_T Cuts at: 0 1100 2747 3495 Size: 1100 1647 748 Fragments arranged by size: 1647 1100 748 BlpI GC'TnA_GC Cuts at: 0 3049 3095 3495 Size: 3049 46 400 Fragments arranged by size: 3049 400 46 Bme1580I G_kGCm'C Cuts at: 0 321 514 652 824 1342 1489 2290 2300 Size: 321 193 138 172 518 147 801 10 Cuts at: 2300 2581 3495 Size: 281 914 Fragments arranged by size: 914 801 518 321 281 193 172 147 138 10 BmgBI CAC'GTC Cuts at: 0 383 1116 1215 3363 3495 Size: 383 733 99 2148 132 Fragments arranged by size: 2148 733 383 132 99 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 735 1978 3495 Size: 735 1243 1517 Fragments arranged by size: 1517 1243 735 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 472 631 705 1159 1702 1836 2050 2359 Size: 472 159 74 454 543 134 214 309 Cuts at: 2359 2725 2839 3136 3334 3495 Size: 366 114 297 198 161 Fragments arranged by size: 543 472 454 366 309 297 214 198 161 159 134 114 74 Bpu10I CC'TnA_GC Cuts at: 0 604 2126 3058 3187 3495 Size: 604 1522 932 129 308 Fragments arranged by size: 1522 932 604 308 129 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1039 1393 3495 Size: 1039 354 2102 Fragments arranged by size: 2102 1039 354 BsaI GGTCTCn'nnnn_ Cuts at: 0 1401 3116 3495 Size: 1401 1715 379 Fragments arranged by size: 1715 1401 379 BsaAI yAC'GTr Cuts at: 0 2146 3495 Size: 2146 1349 BsaBI GATnn'nnATC Cuts at: 0 2551 3495 Size: 2551 944 BsaHI Gr'CG_yC Cuts at: 0 1157 1427 1856 2116 2615 3495 Size: 1157 270 429 260 499 880 Fragments arranged by size: 1157 880 499 429 270 260 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1111 1141 1381 1411 1735 1765 3495 Size: 1111 30 240 30 324 30 1730 Fragments arranged by size: 1730 1111 324 240 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 235 469 985 1156 1324 2116 3222 3495 Size: 235 234 516 171 168 792 1106 273 Fragments arranged by size: 1106 792 516 273 235 234 171 168 BseYI C'CCAG_C Cuts at: 0 60 423 859 1343 2574 2839 3455 3495 Size: 60 363 436 484 1231 265 616 40 Fragments arranged by size: 1231 616 484 436 363 265 60 40 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 421 1372 1819 2668 2974 3495 Size: 421 951 447 849 306 521 Fragments arranged by size: 951 849 521 447 421 306 BsiEI CG_ry'CG Cuts at: 0 1455 2700 3495 Size: 1455 1245 795 Fragments arranged by size: 1455 1245 795 BsiHKAI G_wGCw'C Cuts at: 0 155 295 1055 1253 2729 3101 3113 3344 Size: 155 140 760 198 1476 372 12 231 Cuts at: 3344 3394 3495 Size: 50 101 Fragments arranged by size: 1476 760 372 231 198 155 140 101 50 12 BsmI GAATG_Cn' Cuts at: 0 446 3495 Size: 446 3049 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1678 3495 Size: 1678 1817 Bsp1286I G_dGCh'C Cuts at: 0 155 295 321 514 652 824 967 1055 Size: 155 140 26 193 138 172 143 88 Cuts at: 1055 1253 1342 1489 2074 2127 2290 2300 2581 Size: 198 89 147 585 53 163 10 281 Cuts at: 2581 2729 3101 3113 3344 3394 3495 Size: 148 372 12 231 50 101 Fragments arranged by size: 585 372 281 231 198 193 172 163 155 148 147 143 140 138 101 89 88 53 50 26 12 10 BspHI T'CATG_A Cuts at: 0 2909 3495 Size: 2909 586 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 170 191 941 1298 1837 2045 2476 2750 Size: 170 21 750 357 539 208 431 274 Cuts at: 2750 2924 3134 3495 Size: 174 210 361 Fragments arranged by size: 750 539 431 361 357 274 210 208 174 170 21 BsrBI CCG'CTC Cuts at: 0 134 1259 1731 3495 Size: 134 1125 472 1764 Fragments arranged by size: 1764 1125 472 134 BsrDI GCAATG_nn' Cuts at: 0 3260 3495 Size: 3260 235 BsrFI r'CCGG_y Cuts at: 0 50 186 1330 1374 1454 2732 3495 Size: 50 136 1144 44 80 1278 763 Fragments arranged by size: 1278 1144 763 136 80 50 44 BssSI C'ACGA_G Cuts at: 0 1390 1591 2650 3013 3343 3495 Size: 1390 201 1059 363 330 152 Fragments arranged by size: 1390 1059 363 330 201 152 BstAPI GCAn_nnn'nTGC Cuts at: 0 357 3495 Size: 357 3138 BstEII G'GTnAC_C Cuts at: 0 2131 2304 2877 2892 3495 Size: 2131 173 573 15 603 Fragments arranged by size: 2131 603 573 173 15 BstXI CCAn_nnnn'nTGG Cuts at: 0 201 708 729 2859 3442 3495 Size: 201 507 21 2130 583 53 Fragments arranged by size: 2130 583 507 201 53 21 BstYI r'GATC_y Cuts at: 0 305 665 716 1100 1904 2711 2747 2759 Size: 305 360 51 384 804 807 36 12 Cuts at: 2759 3495 Size: 736 Fragments arranged by size: 807 804 736 384 360 305 51 36 12 Bsu36I CC'TnA_GG Cuts at: 0 488 1034 2017 2292 3495 Size: 488 546 983 275 1203 Fragments arranged by size: 1203 983 546 488 275 BtgI C'CryG_G Cuts at: 0 728 745 1064 1364 1502 1616 2516 3353 Size: 728 17 319 300 138 114 900 837 Cuts at: 3353 3495 Size: 142 Fragments arranged by size: 900 837 728 319 300 142 138 114 17 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 596 759 1071 2907 3495 Size: 596 163 312 1836 588 Fragments arranged by size: 1836 596 588 312 163 BtsI GCAGTG_nn' Cuts at: 0 2809 3495 Size: 2809 686 DraIII CAC_nnn'GTG Cuts at: 0 2385 3252 3495 Size: 2385 867 243 Fragments arranged by size: 2385 867 243 DrdI GACnn_nn'nnGTC Cuts at: 0 1162 3148 3495 Size: 1162 1986 347 Fragments arranged by size: 1986 1162 347 EaeI y'GGCC_r Cuts at: 0 335 374 830 1061 1217 1328 1452 1456 Size: 335 39 456 231 156 111 124 4 Cuts at: 1456 1490 1598 1646 1841 1922 2438 2499 2697 Size: 34 108 48 195 81 516 61 198 Cuts at: 2697 2754 3474 3495 Size: 57 720 21 Fragments arranged by size: 720 516 456 335 231 198 195 156 124 111 108 81 61 57 48 39 34 21 4 EagI C'GGCC_G Cuts at: 0 1452 2697 3495 Size: 1452 1245 798 Fragments arranged by size: 1452 1245 798 EarI CTCTTCn'nnn_ Cuts at: 0 1288 1531 1753 2062 2242 2635 3495 Size: 1288 243 222 309 180 393 860 Fragments arranged by size: 1288 860 393 309 243 222 180 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1492 2187 2707 3061 3495 Size: 1492 695 520 354 434 Fragments arranged by size: 1492 695 520 434 354 EcoICRI GAG'CTC Cuts at: 0 1053 1251 3392 3495 Size: 1053 198 2141 103 Fragments arranged by size: 2141 1053 198 103 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 65 472 631 705 1012 1159 1702 1836 Size: 65 407 159 74 307 147 543 134 Cuts at: 1836 1983 2050 2253 2359 2725 2839 3136 3334 Size: 147 67 203 106 366 114 297 198 Cuts at: 3334 3495 Size: 161 Fragments arranged by size: 543 407 366 307 297 203 198 161 159 147 147 134 114 106 74 67 65 EcoNI CCTnn'n_nnAGG Cuts at: 0 711 1141 1390 3495 Size: 711 430 249 2105 Fragments arranged by size: 2105 711 430 249 EcoO109I rG'GnC_Cy Cuts at: 0 16 510 511 565 1485 1557 2034 2043 Size: 16 494 1 54 920 72 477 9 Cuts at: 2043 2286 2287 2296 2578 3495 Size: 243 1 9 282 917 Fragments arranged by size: 920 917 494 477 282 243 72 54 16 9 9 1 1 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1884 2634 3198 3495 Size: 1884 750 564 297 Fragments arranged by size: 1884 750 564 297 FseI GG_CCGG'CC Cuts at: 0 1334 1458 3495 Size: 1334 124 2037 Fragments arranged by size: 2037 1334 124 FspI TGC'GCA Cuts at: 0 265 3495 Size: 265 3230 HaeII r_GCGC'y Cuts at: 0 1000 2119 2189 2680 2991 3021 3495 Size: 1000 1119 70 491 311 30 474 Fragments arranged by size: 1119 1000 491 474 311 70 30 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 290 322 1111 1143 1735 1767 2815 2847 Size: 290 32 789 32 592 32 1048 32 Cuts at: 2847 3495 Size: 648 Fragments arranged by size: 1048 789 648 592 290 32 32 32 32 HincII GTy'rAC Cuts at: 0 84 3495 Size: 84 3411 KasI G'GCGC_C Cuts at: 0 2115 3495 Size: 2115 1380 MscI TGG'CCA Cuts at: 0 337 376 1600 1843 2440 3476 3495 Size: 337 39 1224 243 597 1036 19 Fragments arranged by size: 1224 1036 597 337 243 39 19 MslI CAynn'nnrTG Cuts at: 0 553 2690 2899 2926 3440 3495 Size: 553 2137 209 27 514 55 Fragments arranged by size: 2137 553 514 209 55 27 MspA1I CmG'CkG Cuts at: 0 1299 1366 1811 2076 2574 3420 3495 Size: 1299 67 445 265 498 846 75 Fragments arranged by size: 1299 846 498 445 265 75 67 NaeI GCC'GGC Cuts at: 0 188 1332 1456 3495 Size: 188 1144 124 2039 Fragments arranged by size: 2039 1144 188 124 NarI GG'CG_CC Cuts at: 0 2116 3495 Size: 2116 1379 NcoI C'CATG_G Cuts at: 0 1616 2516 3495 Size: 1616 900 979 Fragments arranged by size: 1616 979 900 NgoMIV G'CCGG_C Cuts at: 0 186 1330 1454 3495 Size: 186 1144 124 2041 Fragments arranged by size: 2041 1144 186 124 NspI r_CATG'y Cuts at: 0 346 2973 3495 Size: 346 2627 522 Fragments arranged by size: 2627 522 346 PasI CC'CwG_GG Cuts at: 0 2283 3372 3495 Size: 2283 1089 123 Fragments arranged by size: 2283 1089 123 PciI A'CATG_T Cuts at: 0 2969 3495 Size: 2969 526 PflMI CCAn_nnn'nTGG Cuts at: 0 372 816 1596 1907 3252 3423 3495 Size: 372 444 780 311 1345 171 72 Fragments arranged by size: 1345 780 444 372 311 171 72 PfoI T'CCnGG_A Cuts at: 0 24 3495 Size: 24 3471 PmlI CAC'GTG Cuts at: 0 2146 3495 Size: 2146 1349 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 904 936 3495 Size: 904 32 2559 Fragments arranged by size: 2559 904 32 PpuMI rG'GwC_Cy Cuts at: 0 1557 2034 2043 3495 Size: 1557 477 9 1452 Fragments arranged by size: 1557 1452 477 9 PspOMI G'GGCC_C Cuts at: 0 510 1485 2286 2296 2577 3495 Size: 510 975 801 10 281 918 Fragments arranged by size: 975 918 801 510 281 10 PstI C_TGCA'G Cuts at: 0 911 1247 1298 1424 1583 2042 2090 2473 Size: 911 336 51 126 159 459 48 383 Cuts at: 2473 2747 3495 Size: 274 748 Fragments arranged by size: 911 748 459 383 336 274 159 126 51 48 PvuII CAG'CTG Cuts at: 0 2574 3420 3495 Size: 2574 846 75 Fragments arranged by size: 2574 846 75 SacI G_AGCT'C Cuts at: 0 1055 1253 3394 3495 Size: 1055 198 2141 101 Fragments arranged by size: 2141 1055 198 101 SacII CC_GC'GG Cuts at: 0 1367 3495 Size: 1367 2128 SapI GCTCTTCn'nnn_ Cuts at: 0 1288 1531 2062 3495 Size: 1288 243 531 1433 Fragments arranged by size: 1433 1288 531 243 SbfI CC_TGCA'GG Cuts at: 0 1424 2042 2473 3495 Size: 1424 618 431 1022 Fragments arranged by size: 1424 1022 618 431 SexAI A'CCwGG_T Cuts at: 0 197 2381 3495 Size: 197 2184 1114 Fragments arranged by size: 2184 1114 197 SfcI C'TryA_G Cuts at: 0 907 1243 1294 1420 1579 1761 2038 2086 Size: 907 336 51 126 159 182 277 48 Cuts at: 2086 2469 2743 3495 Size: 383 274 752 Fragments arranged by size: 907 752 383 336 277 274 182 159 126 51 48 SfiI GGCCn_nnn'nGGCC Cuts at: 0 1699 3495 Size: 1699 1796 SfoI GGC'GCC Cuts at: 0 2117 3495 Size: 2117 1378 SmaI CCC'GGG Cuts at: 0 36 572 1354 3468 3495 Size: 36 536 782 2114 27 Fragments arranged by size: 2114 782 536 36 27 SmlI C'TyrA_G Cuts at: 0 1054 1408 3495 Size: 1054 354 2087 Fragments arranged by size: 2087 1054 354 StuI AGG'CCT Cuts at: 0 1864 1891 2027 3495 Size: 1864 27 136 1468 Fragments arranged by size: 1864 1468 136 27 StyI C'CwwG_G Cuts at: 0 12 389 707 824 1616 1859 2516 3495 Size: 12 377 318 117 792 243 657 979 Fragments arranged by size: 979 792 657 377 318 243 117 12 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 205 1357 2150 2449 2686 2818 2822 3495 Size: 205 1152 793 299 237 132 4 673 Fragments arranged by size: 1152 793 673 299 237 205 132 4 TatI w'GTAC_w Cuts at: 0 2222 3495 Size: 2222 1273 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1105 1116 1137 1148 1381 1413 2412 2444 Size: 1105 11 21 11 233 32 999 32 Cuts at: 2444 3495 Size: 1051 Fragments arranged by size: 1105 1051 999 233 32 32 21 11 11 Tth111I GACn'n_nGTC Cuts at: 0 982 3495 Size: 982 2513 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 396 2523 2589 3495 Size: 396 2127 66 906 Fragments arranged by size: 2127 906 396 66 XmaI C'CCGG_G Cuts at: 0 34 570 1352 3466 3495 Size: 34 536 782 2114 29 Fragments arranged by size: 2114 782 536 34 29 XmnI GAAnn'nnTTC Cuts at: 0 2392 3495 Size: 2392 1103 ZraI GAC'GTC Cuts at: 0 2616 3495 Size: 2616 879 Enzymes that do cut: AarI AatII AcuI AfeI AflIII AleI AlfI AlwNI ApaI ApoI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BglI BglII BlpI Bme1580I BmgBI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI FseI FspI HaeII Hin4I HincII KasI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI PciI PflMI PfoI PmlI PpiI PpuMI PspOMI PstI PvuII SacI SacII SapI SbfI SexAI SfcI SfiI SfoI SmaI SmlI StuI StyI TaqII TatI TstI Tth111I XcmI XmaI XmnI ZraI Enzymes that do not cut: AccI Acc65I AclI AflII AgeI AhdI AloI ApaLI AscI AseI AsiSI AvrII BaeI BciVI BclI BfrBI BmrI BmtI BsaWI BsiWI BspEI BsrGI BssHII BstBI BstZ17I ClaI CspCI DraI EcoRI EcoRV FspAI HindIII HpaI KpnI MfeI MluI MmeI NdeI NheI NotI NruI NsiI PacI PmeI PshAI PsiI PspXI PsrI PvuI RsrII SalI SanDI ScaI SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI XbaI XhoI