################################## # NO CUT ENZYMES ( 162 enzymes) # ################################## AatII Acc65I AccI AclI AcsI AflII AflIII AgeI AhdI Alw44I AlwNI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AviII AvrII BalI BanI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfaI BfrI BlnI BlpI BpmI Bpu1102I BpuAI BsaBI BsaI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmI Bsp1407I BspDI BspHI BspLU11I BspMI BsrBI BsrDI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstNI BstPI BstXI BstZ17I CelII ClaI CpoI DraI DraII DraIII EaeI EagI Eam1105I EclXI Eco52I EcoNI EcoO109I EcoO65I EcoRI EcoRII EcoRV EcoT22I FbaI FokI FseI FspI HincII HindII HindIII HinfI HpaI HphI KasI KpnI MaeI MaeIII MamI MfeI MluI MluNI MscI MseI MunI MvaI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI RcaI RsrII SacI SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI TfiI Tru9I Tsp45I TspRI Tth111I Van91I XbaI XcmI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AccIII 125 TCCGGA T^CCGGA AcyI 436 GGCGTC G[GA]^CG[CT]C AocI 156 CCTCAGG CC^T[ACGT]AGG Aor51HI 361 AGCGCT AGC^GCT AvaII 153 GGACC G^G[AT]CC BamHI 128 GGATCC G^GATCC BanII 348 GAGCCC G[GA]GC[CT]^C BcnI 91 CCCGG CC^[GC]GG BglI 350 GCCCTGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 324 AGATCT A^GATCT BsaAI 286 TACGTG [CT]AC^GT[GA] BsaHI 436 GGCGTC G[GA]^CG[CT]C BsaWI 125 TCCGGA [AT]^CCGG[AT] BseAI 125 TCCGGA T^CCGGA BsmFI 439 GTCCC GGGAC(10/14) BspEI 125 TCCGGA T^CCGGA BsrFI 29 ACCGGC [GA]^CCGG[CT] BssKI 91 CCCGG ^CC[ACGT]GG Bsu36I 156 CCTCAGG CC^T[ACGT]AGG Cfr10I 29 ACCGGC [GA]^CCGG[CT] DdeI 157 CTCAG C^T[ACGT]AG DrdI 271 GACTACAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 236 GAAGAG CTCTTC(1/4) Eco47III 361 AGCGCT AGC^GCT Eco81I 156 CCTCAGG CC^T[ACGT]AGG HaeII 361 AGCGCT [GA]GCGC^[CT] HgaI 437 GCGTC GACGC(5/10) Hin1I 436 GGCGTC G[GA]^CG[CT]C Ksp632I 236 GAAGAG CTCTTC(1/4) KspI 424 CCGCGG CCGC^GG MroI 125 TCCGGA T^CCGGA MslI 135 CATGGAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NciI 91 CCCGG CC^[GC]GG NlaIV 128 GGATCC GG[ACGT]^[ACGT]CC NspI 383 GCATGC [GA]CATG^[CT] SacII 424 CCGCGG CCGC^GG ScrFI 91 CCCGG CC^[ACGT]GG SfaNI 176 GCATC GCATC(5/9) SfcI 378 CTACAG C^T[GA][CT]AG SphI 383 GCATGC GCATG^C Tsp509I 163 AATT ^AATT ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AccII 5 CGCG CG^CG 425 CGCG AfaI 18 GTAC GT^AC 27 GTAC BmyI 187 GTGCCC G[AGT]GC[ACT]^C 348 GAGCCC BseRI 391 GAGGAG GAGGAG(10/8) 394 GAGGAG Bsp1286I 187 GTGCCC G[AGT]GC[ACT]^C 348 GAGCCC BstUI 5 CGCG CG^CG 425 CGCG BstYI 128 GGATCC [GA]^GATC[CT] 324 AGATCT Cac8I 294 GCTGGC GC[ACGT]^[ACGT]GC 383 GCATGC CfoI 4 GCGC GCG^C 362 GCGC EcoT14I 134 CCATGG C^C[AT][AT]GG 300 CCATGG FauI 423 CCCGC CCCGC(4/6) 429 GCGGG HhaI 4 GCGC GCG^C 362 GCGC HinP1I 4 GCGC G^CGC 362 GCGC MboII 236 GAAGA GAAGA(8/7) 71 TCTTC MflI 128 GGATCC [GA]^GATC[CT] 324 AGATCT MvnI 5 CGCG CG^CG 425 CGCG NcoI 134 CCATGG C^CATGG 300 CCATGG PaeR7I 169 CTCGAG C^TCGAG 388 CTCGAG RsaI 18 GTAC GT^AC 27 GTAC SmlI 169 CTCGAG C^T[CT][GA]AG 388 CTCGAG StyI 134 CCATGG C^C[AT][AT]GG 300 CCATGG TaqI 170 TCGA T^CGA 389 TCGA TthHB8I 170 TCGA T^CGA 389 TCGA XhoI 169 CTCGAG C^TCGAG 388 CTCGAG XhoII 128 GGATCC [GA]^GATC[CT] 324 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 30 enzymes) # ######################################### AciI AluI AlwI AvaI BbvI BsaJI BsgI BsiYI BslI BsoBI Cfr13I DpnII DsaI Fnu4HI HaeIII HapII HpaII ItaI MaeII MboI MnlI MspA1I MspI MwoI NdeII NlaIII PvuII Sau3AI Sau96I TseI