################################## # NO CUT ENZYMES ( 82 enzymes) # ################################## AatII AclI AcsI AflII AgeI AhdI Aor51HI ApaI ApoI AscI AseI AsnI Asp700I AspEI AviII AvrII BbvCI BciVI BclI BfaI BfrI BlnI BsiWI BsmI Bsp1407I BspDI BsrGI Bst1107I BstAPI BstBI BstZ17I ClaI CpoI DraI Eam1105I Eco47III EcoRI EcoRV EcoT22I FbaI FseI FspI HpaI KspI MaeI MfeI MluI MunI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmeI PshAI PshBI Psp1406I PvuI PvuII RsrII SacII SbfI ScaI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tsp509I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### Acc65I 734 GGTACC G^GTACC AccIII 20 TCCGGA T^CCGGA Alw44I 1153 GTGCAC G^TGCAC AocI 289 CCTGAGG CC^T[ACGT]AGG ApaLI 1153 GTGCAC G^TGCAC Asp718I 734 GGTACC G^GTACC BbrPI 2131 CACGTG CAC^GTG BcgI 1466 GCAACTGCGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BlpI 1461 GCTCAGC GC^T[ACGT]AGC Bpu1102I 1461 GCTCAGC GC^T[ACGT]AGC BsaWI 20 TCCGGA [AT]^CCGG[AT] BseAI 20 TCCGGA T^CCGGA BsmBI 1301 CGTCTC CGTCTC(1/5) BspEI 20 TCCGGA T^CCGGA BspLU11I 506 ACATGT A^CATGT BsrFI 606 GCCGGC [GA]^CCGG[CT] BssHII 864 GCGCGC G^CGCGC Bsu36I 289 CCTGAGG CC^T[ACGT]AGG CelII 1461 GCTCAGC GC^T[ACGT]AGC Cfr10I 606 GCCGGC [GA]^CCGG[CT] DraIII 1156 CACTATGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 247 GACAACAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 289 CCTGAGG CC^T[ACGT]AGG EcoNI 456 CCTGCACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG HindIII 1207 AAGCTT A^AGCTT KpnI 734 GGTACC GGTAC^C MroI 20 TCCGGA T^CCGGA NaeI 606 GCCGGC GCC^GGC NgoMIV 606 GCCGGC G^CCGGC PflMI 1044 CCACGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 2131 CACGTG CAC^GTG PmlI 2131 CACGTG CAC^GTG SalI 1930 GTCGAC G^TCGAC SexAI 1481 ACCTGGT A^CC[AT]GGT SmlI 1939 CTCAAG C^T[CT][GA]AG StuI 86 AGGCCT AGG^CCT Van91I 1044 CCACGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AflIII 506 ACATGT A^C[GA][CT]GT 896 ACGTGT AspI 103 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC 430 GACGGGGTC BalI 1433 TGGCCA TGG^CCA 2096 TGGCCA BamHI 23 GGATCC G^GATCC 236 GGATCC BbeI 1680 GGCGCC GGCGC^C 1754 GGCGCC BglII 680 AGATCT A^GATCT 848 AGATCT BsaAI 895 TACGTG [CT]AC^GT[GA] 2131 CACGTG BsrBI 1509 CCGCTC CCGCTC(-3/-3) 639 GAGCGG BsrDI 1830 CATTGC GCAATG(2/0) 1848 CATTGC EcoT14I 1775 CCAAGG C^C[AT][AT]GG 1979 CCAAGG HaeII 1680 GGCGCC [GA]GCGC^[CT] 1754 GGCGCC KasI 1680 GGCGCC G^GCGCC 1754 GGCGCC MluNI 1433 TGGCCA TGG^CCA 2096 TGGCCA MscI 1433 TGGCCA TGG^CCA 2096 TGGCCA MseI 200 TTAA T^TAA 938 TTAA NarI 1680 GGCGCC GG^CGCC 1754 GGCGCC NspI 506 ACATGT [GA]CATG^[CT] 596 ACATGC PflFI 103 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC 430 GACGGGGTC PstI 1279 CTGCAG CTGCA^G 1795 CTGCAG SacI 175 GAGCTC GAGCT^C 1555 GAGCTC SapI 1080 GCTCTTC GCTCTTC(1/4) 1695 GCTCTTC SfoI 1680 GGCGCC GGC^GCC 1754 GGCGCC SmaI 1348 CCCGGG CCC^GGG 1544 CCCGGG StyI 1775 CCAAGG C^C[AT][AT]GG 1979 CCAAGG Tru9I 200 TTAA T^TAA 938 TTAA Tth111I 103 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC 430 GACGGGGTC XmaCI 1348 CCCGGG C^CCGGG 1544 CCCGGG XmaI 1348 CCCGGG C^CCGGG 1544 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 111 enzymes) # ######################################### AccI AccII AciI AcyI AfaI AluI AlwI AlwNI AspHI AvaI AvaII BanI BanII BbsI BbvI BcnI BglI BmyI BpmI BpuAI BsaBI BsaHI BsaI BsaJI BseRI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspHI BspMI BsrI BssKI BssSI BstEII BstF5I BstNI BstPI BstUI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EagI EarI EclXI Eco52I EcoO109I EcoO65I EcoRII FauI Fnu4HI FokI HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeII MaeIII MamI MboI MboII MflI MnlI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI PpuMI RcaI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI TaqI TfiI TseI Tsp45I TspRI TthHB8I XcmI XhoII