################################## # NO CUT ENZYMES ( 132 enzymes) # ################################## AatII AccI AccIII AclI AcsI AflII AgeI AhdI Alw44I AlwI ApaLI ApoI AscI AseI AsnI Asp700I AspEI AspHI AspI AviII AvrII BamHI BbrPI BbvCI BcgI BciVI BclI BfaI BfrI BglII BlnI BsaAI BsaBI BsaWI BseAI BsgI BsiHKAI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrDI BsrGI BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstYI BstZ17I ClaI CpoI DpnII DraIII DrdI Eam1105I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI MaeI MaeII MaeIII MamI MboI MfeI MflI MroI MslI MunI NcoI NdeI NdeII NheI NruI NsiI NspV PacI PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SalI SapI Sau3AI SbfI ScaI SexAI SfuI SgrAI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI TfiI Tsp45I Tsp509I TspRI Tth111I Van91I XbaI XcmI XhoII XmnI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### Acc65I 268 GGTACC G^GTACC AflIII 656 ACGCGT A^C[GA][CT]GT AlwNI 667 CAGACTCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 217 CCTAAGG CC^T[ACGT]AGG Aor51HI 512 AGCGCT AGC^GCT Asp718I 268 GGTACC G^GTACC AvaII 395 GGTCC G^G[AT]CC BalI 118 TGGCCA TGG^CCA BbeI 183 GGCGCC GGCGC^C BbsI 450 GAAGAC GAAGAC(2/6) BpuAI 450 GAAGAC GAAGAC(2/6) BsaI 568 GAGACC GGTCTC(1/5) BsmBI 48 CGTCTC CGTCTC(1/5) BsrI 66 CCAGT ACTGG(1/-1) BssHII 534 GCGCGC G^CGCGC Bsu36I 217 CCTAAGG CC^T[ACGT]AGG DraI 346 TTTAAA TTT^AAA EarI 352 GAAGAG CTCTTC(1/4) Eco47III 512 AGCGCT AGC^GCT Eco81I 217 CCTAAGG CC^T[ACGT]AGG EcoT14I 275 CCAAGG C^C[AT][AT]GG KasI 183 GGCGCC G^GCGCC KpnI 268 GGTACC GGTAC^C Ksp632I 352 GAAGAG CTCTTC(1/4) MluI 656 ACGCGT A^CGCGT MluNI 118 TGGCCA TGG^CCA MscI 118 TGGCCA TGG^CCA MseI 347 TTAA T^TAA NaeI 433 GCCGGC GCC^GGC NarI 183 GGCGCC GG^CGCC NgoMIV 433 GCCGGC G^CCGGC NotI 402 GCGGCCGC GC^GGCCGC NspI 680 GCATGC [GA]CATG^[CT] PaeR7I 490 CTCGAG C^TCGAG PstI 556 CTGCAG CTGCA^G SfaNI 16 GATGC GCATC(5/9) SfcI 556 CTGCAG C^T[GA][CT]AG SfiI 432 GGCCGGCGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 183 GGCGCC GGC^GCC SmlI 490 CTCGAG C^T[CT][GA]AG SphI 680 GCATGC GCATG^C StyI 275 CCAAGG C^C[AT][AT]GG Tru9I 347 TTAA T^TAA XhoI 490 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AcyI 46 GGCGTC G[GA]^CG[CT]C 183 GGCGCC AfaI 269 GTAC GT^AC 483 GTAC BlpI 421 GCTGAGC GC^T[ACGT]AGC 628 GCTGAGC Bpu1102I 421 GCTGAGC GC^T[ACGT]AGC 628 GCTGAGC BsaHI 46 GGCGTC G[GA]^CG[CT]C 183 GGCGCC BsiEI 38 CGGCCG CG[GA][CT]^CG 403 CGGCCG BsmAI 568 GAGAC GTCTC(1/5) 49 GTCTC BsmFI 410 GGGAC GGGAC(10/14) 698 GGGAC CelII 421 GCTGAGC GC^T[ACGT]AGC 628 GCTGAGC EagI 38 CGGCCG C^GGCCG 403 CGGCCG EclXI 38 CGGCCG C^GGCCG 403 CGGCCG Eco52I 38 CGGCCG C^GGCCG 403 CGGCCG Hin1I 46 GGCGTC G[GA]^CG[CT]C 183 GGCGCC HinfI 493 GAGTC G^A[ACGT]TC 669 GACTC HphI 675 GGTGA GGTGA(8/7) 59 TCACC PleI 669 GACTC GAGTC(4/5) 493 GAGTC RsaI 269 GTAC GT^AC 483 GTAC SmaI 297 CCCGGG CCC^GGG 762 CCCGGG XmaCI 297 CCCGGG C^CCGGG 762 CCCGGG XmaI 297 CCCGGG C^CCGGG 762 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 62 enzymes) # ######################################### AccII AciI AluI ApaI AvaI BanI BanII BbvI BcnI BglI BmyI BpmI BsaJI BseRI BsiYI BslI BsoBI Bsp1286I BsrBI BsrFI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI DraII DsaI EaeI EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HpaII ItaI KspI MboII MnlI MspA1I MspI MvaI MvnI MwoI NciI NlaIII NlaIV SacII Sau96I ScrFI TaqI TseI TthHB8I