(Circular) (Six-base) MAPSORT of: pFN21AB8927.seq Check: 1813 from: 1 to: 5524 pFN21AB8927 5524 bp With 182 enzymes: SgfI * March 28, 2012 12:47 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3334 278 Size: 53 83 186 936 1798 2468 Fragments arranged by size: 2468 1798 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2867 1937 Size: 930 4594 Acc65I G'GTAC_C Cuts at: 2273 2845 2273 Size: 572 4952 AclI AA'CG_TT Cuts at: 1563 3651 4024 1563 Size: 2088 373 3063 Fragments arranged by size: 3063 2088 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3587 4971 5239 1632 Size: 1955 1384 268 1917 Fragments arranged by size: 1955 1917 1384 268 AfeI AGC'GCT Cuts at: 2519 2519 Size: 5524 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5327 AflIII A'CryG_T Cuts at: 1203 2661 4423 5400 1203 Size: 1458 1762 977 1327 Fragments arranged by size: 1762 1458 1327 977 AgeI A'CCGG_T Cuts at: 4355 4355 Size: 5524 AhdI GACnn_n'nnGTC Cuts at: 4253 4253 Size: 5524 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5524 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5524 AlwNI CAG_nnn'CTG Cuts at: 2677 3267 4839 2677 Size: 590 1572 3362 Fragments arranged by size: 3362 1572 590 ApaI G_GGCC'C Cuts at: 2093 2237 2303 2093 Size: 144 66 5314 Fragments arranged by size: 5314 144 66 ApaLI G'TGCA_C Cuts at: 3581 4737 3581 Size: 1156 4368 ApoI r'AATT_y Cuts at: 1493 2833 3059 3211 5320 1493 Size: 1340 226 152 2109 1697 Fragments arranged by size: 2109 1697 1340 226 152 AseI AT'TA_AT Cuts at: 160 1032 4078 160 Size: 872 3046 1606 Fragments arranged by size: 3046 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5524 AvaI C'yCGr_G Cuts at: 1870 1943 2296 2302 2495 2767 2849 1870 Size: 73 353 6 193 272 82 4545 Fragments arranged by size: 4545 353 272 193 82 73 6 BamHI G'GATC_C Cuts at: 2854 3221 2854 Size: 367 5157 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2101 2188 2273 2728 2845 Size: 325 217 627 314 87 85 455 117 Cuts at: 2845 4300 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 455 325 314 217 117 87 85 BanII G_rGCy'C Cuts at: 1391 2093 2115 2237 2303 2387 2843 1391 Size: 702 22 122 66 84 456 4072 Fragments arranged by size: 4072 702 456 122 84 66 22 BbeI G_GCGC'C Cuts at: 2192 2192 Size: 5524 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2462 928 Size: 971 563 3990 Fragments arranged by size: 3990 971 563 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3715 3749 1903 Size: 34 1778 34 3678 Fragments arranged by size: 3678 1778 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3416 4626 3416 Size: 1210 4314 BclI T'GATC_A Cuts at: 1548 1548 Size: 5524 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2307 2444 2654 Size: 107 122 71 631 605 635 137 210 Cuts at: 2654 4135 136 Size: 1481 1525 Fragments arranged by size: 1525 1481 635 631 605 210 137 122 107 71 BglII A'GATC_T Cuts at: 5519 5519 Size: 5524 BlpI GC'TnA_GC Cuts at: 2427 2634 2941 2427 Size: 207 307 5010 Fragments arranged by size: 5010 307 207 Bme1580I G_kGCm'C Cuts at: 1790 2093 2237 2303 3585 4741 1790 Size: 303 144 66 1282 1156 2573 Fragments arranged by size: 2573 1282 1156 303 144 66 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5524 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3255 4213 442 Size: 416 53 83 399 24 1838 958 1753 Fragments arranged by size: 1838 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5524 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2041 2539 2660 4184 1239 Size: 802 498 121 1524 2579 Fragments arranged by size: 2579 1524 802 498 121 Bpu10I CC'TnA_GC Cuts at: 2525 2525 Size: 5524 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3649 4514 4812 3649 Size: 865 298 4361 Fragments arranged by size: 4361 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2567 4187 882 Size: 617 1068 1620 2219 Fragments arranged by size: 2219 1620 1068 617 BsaAI yAC'GTr Cuts at: 493 1204 3315 493 Size: 711 2111 2702 Fragments arranged by size: 2702 2111 711 BsaBI GATnn'nnATC Cuts at: 5518 5518 Size: 5524 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2052 2189 3331 3713 Size: 53 83 186 936 519 137 1142 382 Cuts at: 3713 4348 275 Size: 635 1451 Fragments arranged by size: 1451 1142 936 635 519 382 186 137 83 53 BsaWI w'CCGG_w Cuts at: 3957 4355 4629 4776 5187 3957 Size: 398 274 147 411 4294 Fragments arranged by size: 4294 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5299 Fragments arranged by size: 5299 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2174 2177 2180 1186 Size: 988 3 3 4530 Fragments arranged by size: 4530 988 3 3 BseYI C'CCAG_C Cuts at: 4727 5223 4727 Size: 496 5028 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4951 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2046 2411 2974 3735 4763 1540 Size: 275 186 45 365 563 761 1028 2301 Fragments arranged by size: 2301 1028 761 563 365 275 186 45 BsiHKAI G_wGCw'C Cuts at: 3585 3670 4741 3585 Size: 85 1071 4368 Fragments arranged by size: 4368 1071 85 BsmI GAATG_Cn' Cuts at: 3035 3128 3035 Size: 93 5431 BsmBI CGTCTCn'nnnn_ Cuts at: 2059 2059 Size: 5524 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2093 2115 2237 2303 2387 2843 3585 Size: 399 303 22 122 66 84 456 742 Cuts at: 3585 3670 4741 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 456 399 303 122 85 84 66 22 BspEI T'CCGG_A Cuts at: 5187 5187 Size: 5524 BspHI T'CATG_A Cuts at: 3413 5143 3413 Size: 1730 3794 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2879 844 Size: 2035 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2407 2431 2758 3411 1394 Size: 429 584 24 327 653 3507 Fragments arranged by size: 3507 653 584 429 327 24 BsrDI GCAATG_nn' Cuts at: 65 1248 4019 4193 65 Size: 1183 2771 174 1396 Fragments arranged by size: 2771 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2045 2438 2725 4168 4355 1700 Size: 110 235 393 287 1443 187 2869 Fragments arranged by size: 2869 1443 393 287 235 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5524 BssHII G'CGCG_C Cuts at: 1925 2539 1925 Size: 614 4910 BssSI C'ACGA_G Cuts at: 3584 4596 3584 Size: 1012 4512 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5524 BstBI TT'CG_AA Cuts at: 4328 4328 Size: 5524 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5524 BstYI r'GATC_y Cuts at: 1972 2854 3221 3606 3623 5075 5161 5173 5519 Size: 882 367 385 17 1452 86 12 346 Cuts at: 5519 1972 Size: 1977 Fragments arranged by size: 1977 1452 882 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2223 2223 Size: 5524 BtgI C'CryG_G Cuts at: 513 1065 2004 2105 2379 2411 513 Size: 552 939 101 274 32 3626 Fragments arranged by size: 3626 939 552 274 101 32 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4785 Fragments arranged by size: 4785 683 56 BtsI GCAGTG_nn' Cuts at: 3040 3834 3854 3040 Size: 794 20 4710 Fragments arranged by size: 4710 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5524 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5489 DraI TTT'AAA Cuts at: 2353 2828 2353 Size: 475 5049 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4531 809 Size: 507 3215 1802 Fragments arranged by size: 3215 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2043 2123 2408 Size: 54 1425 50 275 18 213 80 285 Cuts at: 2408 2445 2646 2971 3860 5489 8 Size: 37 201 325 889 1629 43 Fragments arranged by size: 1629 1425 889 325 285 275 213 201 80 54 50 43 37 18 EagI C'GGCC_G Cuts at: 1537 1812 2043 2408 2971 1537 Size: 275 231 365 563 4090 Fragments arranged by size: 4090 563 365 275 231 EarI CTCTTCn'nnn_ Cuts at: 1353 2352 3454 1353 Size: 999 1102 3423 Fragments arranged by size: 3423 1102 999 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4111 4485 4631 387 Size: 1064 138 2522 374 146 1280 Fragments arranged by size: 2522 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2041 2539 2660 3587 4184 4971 5239 Size: 393 409 498 121 927 597 787 268 Cuts at: 5239 1239 Size: 1524 Fragments arranged by size: 1524 927 787 597 498 409 393 268 121 EcoO109I rG'GnC_Cy Cuts at: 2089 2090 2233 2234 2089 Size: 1 143 1 5379 Fragments arranged by size: 5379 143 1 1 EcoRI G'AATT_C Cuts at: 2833 3211 2833 Size: 378 5146 FspI TGC'GCA Cuts at: 4030 4030 Size: 5524 HaeII r_GCGC'y Cuts at: 1678 2192 2427 2521 4671 1678 Size: 514 235 94 2150 2531 Fragments arranged by size: 2531 2150 514 235 94 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4165 4197 4239 4271 1373 Size: 32 135 32 2593 32 42 32 2626 Fragments arranged by size: 2626 2593 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2868 3114 669 Size: 988 281 930 246 3079 Fragments arranged by size: 3079 988 930 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5524 HpaI GTT'AAC Cuts at: 3114 3114 Size: 5524 KasI G'GCGC_C Cuts at: 2188 2188 Size: 5524 KpnI G_GTAC'C Cuts at: 2277 2849 2277 Size: 572 4952 MfeI C'AATT_G Cuts at: 3123 3123 Size: 5524 MluI A'CGCG_T Cuts at: 2661 5400 2661 Size: 2739 2785 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4638 4822 1305 Size: 3333 184 2007 Fragments arranged by size: 3333 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2125 10 Size: 54 1425 343 293 3409 Fragments arranged by size: 3409 1425 343 293 54 MslI CAynn'nnrTG Cuts at: 518 1166 3482 3841 4000 518 Size: 648 2316 359 159 2042 Fragments arranged by size: 2316 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2107 2289 2381 2413 2692 2940 3617 4765 Size: 501 182 92 32 279 248 677 1148 Cuts at: 4765 5010 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 501 279 248 245 182 92 32 NaeI GCC'GGC Cuts at: 2440 2440 Size: 5524 NarI GG'CG_CC Cuts at: 2189 2189 Size: 5524 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4033 Fragments arranged by size: 4033 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5524 NgoMIV G'CCGG_C Cuts at: 2438 2438 Size: 5524 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5524 NotI GC'GGCC_GC Cuts at: 2408 2971 2408 Size: 563 4961 NspI r_CATG'y Cuts at: 1132 1229 2689 2882 4427 1132 Size: 97 1460 193 1545 2229 Fragments arranged by size: 2229 1545 1460 193 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5524 PciI A'CATG_T Cuts at: 4423 4423 Size: 5524 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5524 PfoI T'CCnGG_A Cuts at: 2700 2700 Size: 5524 PmeI GTTT'AAAC Cuts at: 2828 2828 Size: 5524 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3593 3625 5106 5138 3593 Size: 32 1481 32 3979 Fragments arranged by size: 3979 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5524 PsiI TTA'TAA Cuts at: 3094 3094 Size: 5524 PspOMI G'GGCC_C Cuts at: 2089 2233 2299 2089 Size: 144 66 5314 Fragments arranged by size: 5314 144 66 PspXI vC'TCGA_Gb Cuts at: 2495 2495 Size: 5524 PstI C_TGCA'G Cuts at: 830 2565 2876 830 Size: 1735 311 3478 Fragments arranged by size: 3478 1735 311 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5524 SacII CC_GC'GG Cuts at: 2108 2382 2414 2108 Size: 274 32 5218 Fragments arranged by size: 5218 274 32 SalI G'TCGA_C Cuts at: 1936 2866 1936 Size: 930 4594 SbfI CC_TGCA'GG Cuts at: 2876 2876 Size: 5524 ScaI AGT'ACT Cuts at: 3772 5484 3772 Size: 1712 3812 SfcI C'TryA_G Cuts at: 826 1045 2561 2872 4007 4688 4879 826 Size: 219 1516 311 1135 681 191 1471 Fragments arranged by size: 1516 1471 1135 681 311 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2444 2444 Size: 5524 SfoI GGC'GCC Cuts at: 2190 2190 Size: 5524 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5524 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5524 SmaI CCC'GGG Cuts at: 1872 2304 2769 2851 1872 Size: 432 465 82 4545 Fragments arranged by size: 4545 465 432 82 SmlI C'TyrA_G Cuts at: 820 1017 1943 2495 3628 4529 4791 820 Size: 197 926 552 1133 901 262 1553 Fragments arranged by size: 1553 1133 926 901 552 262 197 SnaBI TAC'GTA Cuts at: 493 3315 493 Size: 2822 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5524 SphI G_CATG'C Cuts at: 1132 2689 2882 1132 Size: 1557 193 3774 Fragments arranged by size: 3774 1557 193 SrfI GCCC'GGGC Cuts at: 2304 2304 Size: 5524 SspI AAT'ATT Cuts at: 5 52 3448 5 Size: 47 3396 2081 Fragments arranged by size: 3396 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5524 StyI C'CwwG_G Cuts at: 513 1065 2004 2280 2963 513 Size: 552 939 276 683 3074 Fragments arranged by size: 3074 939 683 552 276 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3551 3568 3721 3906 4351 325 Size: 1255 1971 17 153 185 445 1498 Fragments arranged by size: 1971 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3770 5482 96 Size: 274 80 33 51 1443 1793 1712 138 Fragments arranged by size: 1793 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5484 5516 5484 Size: 32 5492 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5524 XbaI T'CTAG_A Cuts at: 2860 2860 Size: 5524 XhoI C'TCGA_G Cuts at: 1943 2495 1943 Size: 552 4972 XmaI C'CCGG_G Cuts at: 1870 2302 2767 2849 1870 Size: 432 465 82 4545 Fragments arranged by size: 4545 465 432 82 XmnI GAAnn'nnTTC Cuts at: 3653 3653 Size: 5524 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3332 276 Size: 53 83 186 936 1798 2468 Fragments arranged by size: 2468 1798 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PspXI PstI PvuI SacII SalI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PpuMI PsrI PvuII RsrII SacI SanDI SapI SexAI SwaI XcmI