################################## # NO CUT ENZYMES ( 112 enzymes) # ################################## Acc65I AclI AcsI AflII AflIII AgeI AhdI Alw44I AlwI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AviII AvrII BamHI BbrPI BcgI BciVI BclI BfrI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaI BsiHKAI BsiWI BspDI BspLU11I BsrBI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstYI BstZ17I CelII ClaI CpoI DraI DrdI Eam1105I EarI Eco47III EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HindIII HpaI KpnI Ksp632I MamI MfeI MflI MluI MseI MunI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RsrII SacI SapI SbfI SexAI SfiI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspI StuI SwaI Tru9I XbaI XcmI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 55 enzymes) # ################################### AatII 1131 GACGTC GACGT^C AccI 1134 GTCGAC GT^[AC][GT]AC AccIII 974 TCCGGA T^CCGGA AlwNI 875 CAGGGGCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 689 CCTCAGG CC^T[ACGT]AGG ApaI 137 GGGCCC GGGCC^C AspI 733 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC BbsI 730 GAAGAC GAAGAC(2/6) BbvCI 1143 CCTCAGC CCTCAGC(-5/-2) BfaI 376 CTAG C^TAG BglI 58 GCCCGCCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpuAI 730 GAAGAC GAAGAC(2/6) BsaWI 974 TCCGGA [AT]^CCGG[AT] BseAI 974 TCCGGA T^CCGGA BsmBI 1168 CGTCTC CGTCTC(1/5) BsmI 557 GCATTC GAATGC(1/-1) Bsp1407I 392 TGTACA T^GTACA BspEI 974 TCCGGA T^CCGGA BspHI 600 TCATGA T^CATGA BspMI 1046 ACCTGC ACCTGC(4/8) BsrDI 747 CATTGC GCAATG(2/0) BsrGI 392 TGTACA T^GTACA Bsu36I 689 CCTCAGG CC^T[ACGT]AGG DpnII 193 GATC ^GATC DraIII 502 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 68 CCGCGG C^C[GA][CT]GG Eco81I 689 CCTCAGG CC^T[ACGT]AGG EcoT14I 671 CCAAGG C^C[AT][AT]GG HincII 1134 GTCGAC GT[CT]^[GA]AC HindII 1134 GTCGAC GT[CT]^[GA]AC KspI 68 CCGCGG CCGC^GG MaeI 376 CTAG C^TAG MboI 193 GATC ^GATC MroI 974 TCCGGA T^CCGGA NaeI 695 GCCGGC GCC^GGC NdeII 193 GATC ^GATC NgoMIV 695 GCCGGC G^CCGGC NspI 131 ACATGC [GA]CATG^[CT] PflFI 733 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC PstI 1027 CTGCAG CTGCA^G RcaI 600 TCATGA T^CATGA SacII 68 CCGCGG CCGC^GG SalI 1134 GTCGAC G^TCGAC Sau3AI 193 GATC ^GATC ScaI 590 AGTACT AGT^ACT SfcI 1027 CTGCAG C^T[GA][CT]AG SmaI 215 CCCGGG CCC^GGG SspBI 392 TGTACA T^GTACA StyI 671 CCAAGG C^C[AT][AT]GG TaqI 1135 TCGA T^CGA Tsp509I 409 AATT ^AATT Tth111I 733 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 1135 TCGA T^CGA XmaCI 215 CCCGGG C^CCGGG XmaI 215 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AccII 69 CGCG CG^CG 242 CGCG AvaI 44 CTCGGG C^[CT]CG[GA]G 215 CCCGGG BalI 512 TGGCCA TGG^CCA 1052 TGGCCA BanII 137 GGGCCC G[GA]GC[CT]^C 1000 GAGCCC BbeI 86 GGCGCC GGCGC^C 203 GGCGCC BpmI 654 CTCCAG CTGGAG(16/14) 306 CTGGAG BsgI 1114 CTGCAC GTGCAG(16/14) 533 GTGCAG BsoBI 44 CTCGGG C^[CT]CG[GA]G 215 CCCGGG BsrFI 695 GCCGGC [GA]^CCGG[CT] 1094 ACCGGC BstUI 69 CGCG CG^CG 242 CGCG Cfr10I 695 GCCGGC [GA]^CCGG[CT] 1094 ACCGGC EcoNI 90 CCTGCCGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 150 CCTGGCCCAGG KasI 86 GGCGCC G^GCGCC 203 GGCGCC MaeIII 778 GTGAC ^GT[ACGT]AC 863 GTGAC MboII 549 GAAGA GAAGA(8/7) 730 GAAGA MluNI 512 TGGCCA TGG^CCA 1052 TGGCCA MscI 512 TGGCCA TGG^CCA 1052 TGGCCA MslI 639 CATCATTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1183 CATCTCTATG MvnI 69 CGCG CG^CG 242 CGCG NarI 86 GGCGCC GG^CGCC 203 GGCGCC PpuMI 824 GGGACCT [GA]G^G[AT]CC[CT] 956 GGGACCT PshAI 264 GACCAGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 1093 GACCGGCGTC PvuII 186 CAGCTG CAG^CTG 841 CAGCTG SfoI 86 GGCGCC GGC^GCC 203 GGCGCC Tsp45I 778 GTGAC ^GT[GC]AC 863 GTGAC ######################################### # 3 OR MORE CUT ENZYMES ( 66 enzymes) # ######################################### AciI AcyI AfaI AluI AvaII BanI BbvI BcnI BmyI BsaHI BsaJI BseRI BsiEI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DraII EaeI EagI EclXI Eco52I EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI MaeII MnlI MspA1I MspI MvaI MwoI NciI NlaIII NlaIV PflMI PleI RsaI Sau96I ScrFI SfaNI TfiI TseI TspRI Van91I