(Circular) (Six-base) MAPSORT of: pFN21AE4078.seq Check: 6220 from: 1 to: 5941 pFN21AE4078 5941 bp With 182 enzymes: SgfI * March 28, 2012 13:10 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3060 3060 Size: 5941 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3140 3751 278 Size: 53 83 186 936 1604 611 2468 Fragments arranged by size: 2468 1604 936 611 186 83 53 AccI GT'mk_AC Cuts at: 1937 3140 3284 1937 Size: 1203 144 4594 Fragments arranged by size: 4594 1203 144 Acc65I G'GTAC_C Cuts at: 3262 3262 Size: 5941 AclI AA'CG_TT Cuts at: 1563 4068 4441 1563 Size: 2505 373 3063 Fragments arranged by size: 3063 2505 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2366 2754 4004 5388 5656 1632 Size: 734 388 1250 1384 268 1917 Fragments arranged by size: 1917 1384 1250 734 388 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5744 AflIII A'CryG_T Cuts at: 1203 4840 5817 1203 Size: 3637 977 1327 Fragments arranged by size: 3637 1327 977 AgeI A'CCGG_T Cuts at: 4772 4772 Size: 5941 AhdI GACnn_n'nnGTC Cuts at: 4670 4670 Size: 5941 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5941 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2381 3068 3210 1184 Size: 1197 687 142 3915 Fragments arranged by size: 3915 1197 687 142 AlwNI CAG_nnn'CTG Cuts at: 2885 3684 5256 2885 Size: 799 1572 3570 Fragments arranged by size: 3570 1572 799 ApaI G_GGCC'C Cuts at: 2146 2146 Size: 5941 ApaLI G'TGCA_C Cuts at: 3998 5154 3998 Size: 1156 4785 ApoI r'AATT_y Cuts at: 1493 3250 3476 3628 5737 1493 Size: 1757 226 152 2109 1697 Fragments arranged by size: 2109 1757 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4495 160 Size: 872 3463 1606 Fragments arranged by size: 3463 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5941 AvaI C'yCGr_G Cuts at: 1870 1943 2049 2220 3266 1870 Size: 73 106 171 1046 4545 Fragments arranged by size: 4545 1046 171 106 73 BamHI G'GATC_C Cuts at: 3271 3638 3271 Size: 367 5574 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2091 2208 2255 2447 3262 Size: 325 217 627 304 117 47 192 815 Cuts at: 3262 4717 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 815 627 325 304 217 192 117 47 BanII G_rGCy'C Cuts at: 1391 2146 3009 3260 1391 Size: 755 863 251 4072 Fragments arranged by size: 4072 863 755 251 BbeI G_GCGC'C Cuts at: 2095 2212 2095 Size: 117 5824 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2742 928 Size: 971 843 4127 Fragments arranged by size: 4127 971 843 BbvCI CC'TCA_GC Cuts at: 3149 3149 Size: 5941 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4132 4166 1903 Size: 34 2195 34 3678 Fragments arranged by size: 3678 2195 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3833 5043 3833 Size: 1210 4731 BclI T'GATC_A Cuts at: 1548 1548 Size: 5941 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2069 4552 Size: 107 122 71 631 605 337 60 2483 Cuts at: 4552 136 Size: 1525 Fragments arranged by size: 2483 1525 631 605 337 122 107 71 60 BglII A'GATC_T Cuts at: 5936 5936 Size: 5941 BlpI GC'TnA_GC Cuts at: 3358 3358 Size: 5941 Bme1580I G_kGCm'C Cuts at: 1790 2146 2808 4002 5158 1790 Size: 356 662 1194 1156 2573 Fragments arranged by size: 2573 1194 1156 662 356 BmgBI CAC'GTC Cuts at: 1318 3220 1318 Size: 1902 4039 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2273 2327 2968 Size: 416 53 83 399 24 856 54 641 Cuts at: 2968 3233 3672 4630 442 Size: 265 439 958 1753 Fragments arranged by size: 1753 958 856 641 439 416 399 265 83 54 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5941 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2332 2644 4601 1239 Size: 1093 312 1957 2579 Fragments arranged by size: 2579 1957 1093 312 Bpu10I CC'TnA_GC Cuts at: 3149 3149 Size: 5941 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4066 4931 5229 4066 Size: 865 298 4778 Fragments arranged by size: 4778 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4604 882 Size: 617 3105 2219 Fragments arranged by size: 3105 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3732 493 Size: 711 2528 2702 Fragments arranged by size: 2702 2528 711 BsaBI GATnn'nnATC Cuts at: 5935 5935 Size: 5941 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2092 2209 3103 3137 Size: 53 83 186 936 559 117 894 34 Cuts at: 3137 3748 4130 4765 275 Size: 611 382 635 1451 Fragments arranged by size: 1451 936 894 635 611 559 382 186 117 83 53 34 BsaWI w'CCGG_w Cuts at: 2979 4374 4772 5046 5193 5604 2979 Size: 1395 398 274 147 411 3316 Fragments arranged by size: 3316 1395 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2344 2374 3127 3157 1178 Size: 30 165 30 941 30 753 30 3962 Fragments arranged by size: 3962 941 753 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2039 2042 2045 2048 2051 2054 2117 3017 Size: 853 3 3 3 3 3 63 900 Cuts at: 3017 1186 Size: 4110 Fragments arranged by size: 4110 900 853 63 3 3 3 3 3 BseYI C'CCAG_C Cuts at: 2483 2704 2885 5144 5640 2483 Size: 221 181 2259 496 2784 Fragments arranged by size: 2784 2259 496 221 181 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2559 3104 1163 Size: 573 823 545 4000 Fragments arranged by size: 4000 823 573 545 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2019 2073 2152 3391 4152 5180 Size: 275 186 18 54 79 1239 761 1028 Cuts at: 5180 1540 Size: 2301 Fragments arranged by size: 2301 1239 1028 761 275 186 79 54 18 BsiHKAI G_wGCw'C Cuts at: 4002 4087 5158 4002 Size: 85 1071 4785 Fragments arranged by size: 4785 1071 85 BsmI GAATG_Cn' Cuts at: 2562 3452 3545 2562 Size: 890 93 4958 Fragments arranged by size: 4958 890 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3179 3179 Size: 5941 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2146 2808 3009 3260 4002 4087 5158 Size: 399 356 662 201 251 742 85 1071 Cuts at: 5158 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 662 399 356 251 201 85 BspEI T'CCGG_A Cuts at: 2979 5604 2979 Size: 2625 3316 BspHI T'CATG_A Cuts at: 2605 3830 5560 2605 Size: 1225 1730 2986 Fragments arranged by size: 2986 1730 1225 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3060 3296 844 Size: 2216 236 3489 Fragments arranged by size: 3489 2216 236 BsrBI CCG'CTC Cuts at: 1394 1823 3828 1394 Size: 429 2005 3507 Fragments arranged by size: 3507 2005 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2751 4436 4610 65 Size: 1183 1503 1685 174 1396 Fragments arranged by size: 1685 1503 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2700 3099 4585 4772 1700 Size: 110 890 399 1486 187 2869 Fragments arranged by size: 2869 1486 890 399 187 110 BsrGI T'GTAC_A Cuts at: 96 2397 96 Size: 2301 3640 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5941 BssSI C'ACGA_G Cuts at: 4001 5013 4001 Size: 1012 4929 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5941 BstBI TT'CG_AA Cuts at: 4745 4745 Size: 5941 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5941 BstYI r'GATC_y Cuts at: 1972 3271 3638 4023 4040 5492 5578 5590 5936 Size: 1299 367 385 17 1452 86 12 346 Cuts at: 5936 1972 Size: 1977 Fragments arranged by size: 1977 1452 1299 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2695 2695 Size: 5941 BtgI C'CryG_G Cuts at: 513 1065 2004 2073 513 Size: 552 939 69 4381 Fragments arranged by size: 4381 939 552 69 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5202 Fragments arranged by size: 5202 683 56 BtsI GCAGTG_nn' Cuts at: 2717 2872 2921 3457 4251 4271 2717 Size: 155 49 536 794 20 4387 Fragments arranged by size: 4387 794 536 155 49 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5941 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5906 DraI TTT'AAA Cuts at: 3245 3245 Size: 5941 DraIII CAC_nnn'GTG Cuts at: 2512 2512 Size: 5941 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4948 809 Size: 507 3632 1802 Fragments arranged by size: 3632 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2016 2070 2149 Size: 54 1425 50 275 18 186 54 79 Cuts at: 2149 2517 2673 3057 3388 4277 5906 8 Size: 368 156 384 331 889 1629 43 Fragments arranged by size: 1629 1425 889 384 368 331 275 186 156 79 54 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2016 2070 2149 3388 1537 Size: 275 204 54 79 1239 4090 Fragments arranged by size: 4090 1239 275 204 79 54 EarI CTCTTCn'nnn_ Cuts at: 1353 3871 1353 Size: 2518 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4528 4902 5048 387 Size: 1064 138 2939 374 146 1280 Fragments arranged by size: 2939 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2332 2366 2644 2754 4004 4601 5388 Size: 393 700 34 278 110 1250 597 787 Cuts at: 5388 5656 1239 Size: 268 1524 Fragments arranged by size: 1524 1250 787 700 597 393 278 268 110 34 EcoNI CCTnn'n_nnAGG Cuts at: 2099 2159 2099 Size: 60 5881 EcoO109I rG'GnC_Cy Cuts at: 2078 2143 2830 2962 3111 2078 Size: 65 687 132 149 4908 Fragments arranged by size: 4908 687 149 132 65 EcoRI G'AATT_C Cuts at: 3250 3628 3250 Size: 378 5563 FspI TGC'GCA Cuts at: 4447 4447 Size: 5941 HaeII r_GCGC'y Cuts at: 1678 2095 2185 2212 2326 5088 1678 Size: 417 90 27 114 2762 2531 Fragments arranged by size: 2762 2531 417 114 90 27 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2344 2376 3127 3159 4582 Size: 32 135 32 772 32 751 32 1423 Cuts at: 4582 4614 4656 4688 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1423 772 751 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3141 3285 3531 669 Size: 988 281 1203 144 246 3079 Fragments arranged by size: 3079 1203 988 281 246 144 HindIII A'AGCT_T Cuts at: 748 748 Size: 5941 HpaI GTT'AAC Cuts at: 3531 3531 Size: 5941 KasI G'GCGC_C Cuts at: 2091 2208 2091 Size: 117 5824 KpnI G_GTAC'C Cuts at: 3266 3266 Size: 5941 MfeI C'AATT_G Cuts at: 3540 3540 Size: 5941 MluI A'CGCG_T Cuts at: 5817 5817 Size: 5941 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5055 5239 1305 Size: 3750 184 2007 Fragments arranged by size: 3750 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2519 3059 10 Size: 54 1425 343 687 540 2892 Fragments arranged by size: 2892 1425 687 540 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2648 3192 3899 4258 4417 518 Size: 648 1482 544 707 359 159 2042 Fragments arranged by size: 2042 1482 707 648 544 359 159 MspA1I CmG'CkG Cuts at: 1606 2075 2172 2193 2848 3133 3357 4034 5182 Size: 469 97 21 655 285 224 677 1148 Cuts at: 5182 5427 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 655 469 285 245 224 97 21 NaeI GCC'GGC Cuts at: 2702 2702 Size: 5941 NarI GG'CG_CC Cuts at: 2092 2209 2092 Size: 117 5824 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4450 Fragments arranged by size: 4450 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5941 NgoMIV G'CCGG_C Cuts at: 2700 2700 Size: 5941 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5941 NotI GC'GGCC_GC Cuts at: 3388 3388 Size: 5941 NspI r_CATG'y Cuts at: 1132 1229 2140 3299 4844 1132 Size: 97 911 1159 1545 2229 Fragments arranged by size: 2229 1545 1159 911 97 PasI CC'CwG_GG Cuts at: 1348 2879 1348 Size: 1531 4410 PciI A'CATG_T Cuts at: 4840 4840 Size: 5941 PflMI CCAn_nnn'nTGG Cuts at: 1108 2682 2885 3055 3223 1108 Size: 1574 203 170 168 3826 Fragments arranged by size: 3826 1574 203 170 168 PfoI T'CCnGG_A Cuts at: 3222 3222 Size: 5941 PmeI GTTT'AAAC Cuts at: 3245 3245 Size: 5941 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4010 4042 5523 5555 4010 Size: 32 1481 32 4396 Fragments arranged by size: 4396 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2830 2962 2830 Size: 132 5809 PshAI GACnn'nnGTC Cuts at: 1613 2273 3102 1613 Size: 660 829 4452 Fragments arranged by size: 4452 829 660 PsiI TTA'TAA Cuts at: 3511 3511 Size: 5941 PspOMI G'GGCC_C Cuts at: 2142 2142 Size: 5941 PstI C_TGCA'G Cuts at: 830 3036 3293 830 Size: 2206 257 3478 Fragments arranged by size: 3478 2206 257 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5941 PvuII CAG'CTG Cuts at: 2193 2848 2193 Size: 655 5286 SacII CC_GC'GG Cuts at: 2076 2076 Size: 5941 SalI G'TCGA_C Cuts at: 1936 3139 3283 1936 Size: 1203 144 4594 Fragments arranged by size: 4594 1203 144 SbfI CC_TGCA'GG Cuts at: 3293 3293 Size: 5941 ScaI AGT'ACT Cuts at: 2597 4189 5901 2597 Size: 1592 1712 2637 Fragments arranged by size: 2637 1712 1592 SfcI C'TryA_G Cuts at: 826 1045 3032 3289 4424 5105 5296 826 Size: 219 1987 257 1135 681 191 1471 Fragments arranged by size: 1987 1471 1135 681 257 219 191 SfoI GGC'GCC Cuts at: 2093 2210 2093 Size: 117 5824 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5941 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5941 SmaI CCC'GGG Cuts at: 1872 2222 3268 1872 Size: 350 1046 4545 Fragments arranged by size: 4545 1046 350 SmlI C'TyrA_G Cuts at: 820 1017 1943 4045 4946 5208 820 Size: 197 926 2102 901 262 1553 Fragments arranged by size: 2102 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3732 493 Size: 3239 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5941 SphI G_CATG'C Cuts at: 1132 3299 1132 Size: 2167 3774 SrfI GCCC'GGGC Cuts at: 2222 2222 Size: 5941 SspI AAT'ATT Cuts at: 5 52 3865 5 Size: 47 3813 2081 Fragments arranged by size: 3813 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5941 StyI C'CwwG_G Cuts at: 513 1065 2004 2676 3380 513 Size: 552 939 672 704 3074 Fragments arranged by size: 3074 939 704 672 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2754 3968 3985 4138 4323 4768 325 Size: 1255 1174 1214 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1214 1174 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2397 2595 3024 Size: 274 80 33 51 1443 420 198 429 Cuts at: 3024 3162 4187 5899 96 Size: 138 1025 1712 138 Fragments arranged by size: 1712 1443 1025 429 420 274 198 138 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5901 5933 5901 Size: 32 5909 Tth111I GACn'n_nGTC Cuts at: 1316 2741 1316 Size: 1425 4516 XbaI T'CTAG_A Cuts at: 3277 3277 Size: 5941 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5941 XmaI C'CCGG_G Cuts at: 1870 2220 3266 1870 Size: 350 1046 4545 Fragments arranged by size: 4545 1046 350 XmnI GAAnn'nnTTC Cuts at: 4070 4070 Size: 5941 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3138 3749 276 Size: 53 83 186 936 1604 611 2468 Fragments arranged by size: 2468 1604 936 611 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI BstXI BstZ17I EcoICRI EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SanDI SapI SexAI SfiI SwaI XcmI