Restriction map for
pFN21AE9424
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# NO CUT ENZYMES ( 126 enzymes) #
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Acc65I AccI AccIII AclI AflII
AgeI AhdI AluI AlwNI AocI
AseI AsnI Asp700I Asp718I AspEI
AvaI AvrII BalI BamHI BbrPI
BbvCI BcgI BciVI BfaI BfrI
BglI BlnI BlpI Bpu1102I BsaBI
BsaWI BseAI BsiWI BsmBI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrDI BsrGI Bst1107I BstBI BstEII
BstPI BstXI BstZ17I Bsu36I CelII
ClaI CpoI DraI DraIII EagI
Eam1105I EclXI Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FseI HincII HindII HindIII HpaI
KpnI MaeI MamI MfeI MluI
MluNI MroI MscI MslI MunI
NaeI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacI SalI
SbfI ScaI SfiI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StyI SwaI
TfiI XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AatII 88 GACGTC GACGT^C
AfaI 232 GTAC GT^AC
AflIII 173 ACGTGT A^C[GA][CT]GT
Alw44I 421 GTGCAC G^TGCAC
Aor51HI 268 AGCGCT AGC^GCT
ApaI 318 GGGCCC GGGCC^C
ApaLI 421 GTGCAC G^TGCAC
AscI 195 GGCGCGCC GG^CGCGCC
AspI 883 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 41 TGCGCA TGC^GCA
BbeI 556 GGCGCC GGCGC^C
BbsI 523 GTCTTC GAAGAC(2/6)
BclI 671 TGATCA T^GATCA
BcnI 553 CCCGG CC^[GC]GG
BglII 611 AGATCT A^GATCT
BpuAI 523 GTCTTC GAAGAC(2/6)
BsaAI 346 TACGTG [CT]AC^GT[GA]
BsaI 810 GGTCTC GGTCTC(1/5)
BsgI 975 GTGCAG GTGCAG(16/14)
BsiEI 500 CGGTCG CG[GA][CT]^CG
BsmI 10 GAATGC GAATGC(1/-1)
BsrFI 104 ACCGGC [GA]^CCGG[CT]
BsrI 660 ACTGG ACTGG(1/-1)
BssSI 615 CTCGTG CACGAG(-5/-1)
Cfr10I 104 ACCGGC [GA]^CCGG[CT]
DrdI 1042 GACACATCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 268 AGCGCT AGC^GCT
FbaI 671 TGATCA T^GATCA
FspI 41 TGCGCA TGC^GCA
HinfI 533 GAGTC G^A[ACGT]TC
KasI 556 GGCGCC G^GCGCC
MseI 1036 TTAA T^TAA
NarI 556 GGCGCC GG^CGCC
NciI 553 CCCGG CC^[GC]GG
PflFI 883 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 102 CCACCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 533 GAGTC GAGTC(4/5)
PpuMI 444 GGGTCCT [GA]G^G[AT]CC[CT]
PstI 427 CTGCAG CTGCA^G
RsaI 232 GTAC GT^AC
SexAI 884 ACCTGGT A^CC[AT]GGT
SfcI 427 CTGCAG C^T[GA][CT]AG
SfoI 556 GGCGCC GGC^GCC
StuI 305 AGGCCT AGG^CCT
Tru9I 1036 TTAA T^TAA
Tsp45I 370 GTCAC ^GT[GC]AC
Tth111I 883 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 102 CCACCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 374 CCATCCTCACGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 20 enzymes) #
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AlwI 756 GATCC GGATC(4/5)
864 GATCC
AspHI 142 GTGCTC G[AT]GC[AT]^C
421 GTGCAC
AvaII 252 GGACC G^G[AT]CC
445 GGTCC
BanI 556 GGCGCC G^G[CT][GA]CC
651 GGTGCC
BanII 318 GGGCCC G[GA]GC[CT]^C
831 GAGCCC
BseRI 802 CTCCTC GAGGAG(10/8)
853 CTCCTC
BsiHKAI 142 GTGCTC G[AT]GC[AT]^C
421 GTGCAC
BsrBI 506 CCGCTC CCGCTC(-3/-3)
360 GAGCGG
BssHII 14 GCGCGC G^CGCGC
196 GCGCGC
BstAPI 170 GCAACGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
407 GCATGGTGTGC
BstYI 611 AGATCT [GA]^GATC[CT]
755 AGATCC
DdeI 302 CTGAG C^T[ACGT]AG
358 CTGAG
DraII 318 GGGCCCC [GA]G^G[ACGT]CC[CT]
444 GGGTCCT
EcoO109I 318 GGGCCCC [GA]G^G[ACGT]CC[CT]
444 GGGTCCT
HphI 371 TCACC GGTGA(8/7)
845 TCACC
MflI 611 AGATCT [GA]^GATC[CT]
755 AGATCC
SfaNI 416 GCATC GCATC(5/9)
706 GCATC
TaqI 574 TCGA T^CGA
1069 TCGA
TthHB8I 574 TCGA T^CGA
1069 TCGA
XhoII 611 AGATCT [GA]^GATC[CT]
755 AGATCC
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# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AccII AciI AcsI AcyI ApoI
BbvI BmyI BpmI BsaHI BsaJI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr13I DpnII DsaI
EaeI EarI EcoRII FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HpaII ItaI
Ksp632I KspI MaeII MaeIII MboI
MboII MnlI MspA1I MspI MvaI
MvnI MwoI NdeII NlaIII NlaIV
SacII SapI Sau3AI Sau96I ScrFI
TseI Tsp509I TspRI