(Circular) (Six-base) MAPSORT of: pFN21AE9424.seq Check: 1620 from: 1 to: 5788 pFN21AE9424 5788 bp With 182 enzymes: SgfI * December 28, 2011 15:23 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2349 2439 2349 Size: 90 5698 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2097 3598 278 Size: 53 83 186 936 561 1501 2468 Fragments arranged by size: 2468 1501 936 561 186 83 53 AccI GT'mk_AC Cuts at: 1937 3131 1937 Size: 1194 4594 Acc65I G'GTAC_C Cuts at: 3109 3109 Size: 5788 AclI AA'CG_TT Cuts at: 1563 3915 4288 1563 Size: 2352 373 3063 Fragments arranged by size: 3063 2352 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3851 5235 5503 1632 Size: 2219 1384 268 1917 Fragments arranged by size: 2219 1917 1384 268 AfeI AGC'GCT Cuts at: 2275 2275 Size: 5788 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5591 AflIII A'CryG_T Cuts at: 1203 2178 4687 5664 1203 Size: 975 2509 977 1327 Fragments arranged by size: 2509 1327 977 975 AgeI A'CCGG_T Cuts at: 4619 4619 Size: 5788 AhdI GACnn_n'nnGTC Cuts at: 4517 4517 Size: 5788 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5788 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5788 AlwNI CAG_nnn'CTG Cuts at: 3531 5103 3531 Size: 1572 4216 ApaI G_GGCC'C Cuts at: 2327 2327 Size: 5788 ApaLI G'TGCA_C Cuts at: 2426 3845 5001 2426 Size: 1419 1156 3213 Fragments arranged by size: 3213 1419 1156 ApoI r'AATT_y Cuts at: 1493 2574 2691 3000 3097 3323 3475 5584 1493 Size: 1081 117 309 97 226 152 2109 1697 Fragments arranged by size: 2109 1697 1081 309 226 152 117 97 AscI GG'CGCG_CC Cuts at: 2201 2201 Size: 5788 AseI AT'TA_AT Cuts at: 160 1032 4342 160 Size: 872 3310 1606 Fragments arranged by size: 3310 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5788 AvaI C'yCGr_G Cuts at: 1870 1943 3113 1870 Size: 73 1170 4545 Fragments arranged by size: 4545 1170 73 BamHI G'GATC_C Cuts at: 3118 3485 3118 Size: 367 5421 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2561 2656 3109 4564 618 Size: 325 217 627 774 95 453 1455 1842 Fragments arranged by size: 1842 1455 774 627 453 325 217 95 BanII G_rGCy'C Cuts at: 1391 2327 2840 3107 1391 Size: 936 513 267 4072 Fragments arranged by size: 4072 936 513 267 BbeI G_GCGC'C Cuts at: 2565 2565 Size: 5788 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2521 928 Size: 971 622 4195 Fragments arranged by size: 4195 971 622 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3979 4013 1903 Size: 34 2042 34 3678 Fragments arranged by size: 3678 2042 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3680 4890 3680 Size: 1210 4578 BclI T'GATC_A Cuts at: 1548 2676 1548 Size: 1128 4660 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4399 136 Size: 107 122 71 631 605 2727 1525 Fragments arranged by size: 2727 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2616 5783 2616 Size: 3167 2621 BlpI GC'TnA_GC Cuts at: 3205 3205 Size: 5788 Bme1580I G_kGCm'C Cuts at: 1790 2327 2430 3849 5005 1790 Size: 537 103 1419 1156 2573 Fragments arranged by size: 2573 1419 1156 537 103 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5788 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3519 4477 442 Size: 416 53 83 399 24 2102 958 1753 Fragments arranged by size: 2102 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5788 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2075 2426 2631 4448 1239 Size: 836 351 205 1817 2579 Fragments arranged by size: 2579 1817 836 351 205 Bpu10I CC'TnA_GC Cuts at: 2363 2363 Size: 5788 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3913 4778 5076 3913 Size: 865 298 4625 Fragments arranged by size: 4625 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2821 4451 882 Size: 617 1322 1630 2219 Fragments arranged by size: 2219 1630 1322 617 BsaAI yAC'GTr Cuts at: 493 1204 2353 3579 493 Size: 711 1149 1226 2702 Fragments arranged by size: 2702 1226 1149 711 BsaBI GATnn'nnATC Cuts at: 5782 5782 Size: 5788 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2094 2212 2562 3595 Size: 53 83 186 936 561 118 350 1033 Cuts at: 3595 3977 4612 275 Size: 382 635 1451 Fragments arranged by size: 1451 1033 936 635 561 382 350 186 118 83 53 BsaWI w'CCGG_w Cuts at: 4221 4619 4893 5040 5451 4221 Size: 398 274 147 411 4558 Fragments arranged by size: 4558 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5563 Fragments arranged by size: 5563 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2798 2849 1186 Size: 1612 51 4125 Fragments arranged by size: 4125 1612 51 BseYI C'CCAG_C Cuts at: 2170 2320 2772 4991 5487 2170 Size: 150 452 2219 496 2471 Fragments arranged by size: 2471 2219 496 452 150 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3001 1163 Size: 573 1265 3950 Fragments arranged by size: 3950 1265 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2508 3238 3999 5027 1540 Size: 275 186 507 730 761 1028 2301 Fragments arranged by size: 2301 1028 761 730 507 275 186 BsiHKAI G_wGCw'C Cuts at: 2151 2430 3849 3934 5005 2151 Size: 279 1419 85 1071 2934 Fragments arranged by size: 2934 1419 1071 279 85 BsmI GAATG_Cn' Cuts at: 2021 3299 3392 2021 Size: 1278 93 4417 Fragments arranged by size: 4417 1278 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2151 2327 2430 2840 3107 3849 3934 Size: 399 361 176 103 410 267 742 85 Cuts at: 3934 5005 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 410 399 361 267 176 103 85 BspEI T'CCGG_A Cuts at: 5451 5451 Size: 5788 BspHI T'CATG_A Cuts at: 3677 5407 3677 Size: 1730 4058 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2268 2349 2439 3143 844 Size: 1424 81 90 704 3489 Fragments arranged by size: 3489 1424 704 90 81 BsrBI CCG'CTC Cuts at: 1394 1823 2367 2513 3675 1394 Size: 429 544 146 1162 3507 Fragments arranged by size: 3507 1162 544 429 146 BsrDI GCAATG_nn' Cuts at: 65 1248 4283 4457 65 Size: 1183 3035 174 1396 Fragments arranged by size: 3035 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2109 4432 4619 1700 Size: 110 299 2323 187 2869 Fragments arranged by size: 2869 2323 299 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5788 BssHII G'CGCG_C Cuts at: 1925 2019 2201 1925 Size: 94 182 5512 Fragments arranged by size: 5512 182 94 BssSI C'ACGA_G Cuts at: 2620 3848 4860 2620 Size: 1228 1012 3548 Fragments arranged by size: 3548 1228 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2181 2418 794 Size: 1387 237 4164 Fragments arranged by size: 4164 1387 237 BstBI TT'CG_AA Cuts at: 4592 4592 Size: 5788 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5788 BstYI r'GATC_y Cuts at: 1972 2616 2760 3118 3485 3870 3887 5339 5425 Size: 644 144 358 367 385 17 1452 86 Cuts at: 5425 5437 5783 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 644 385 367 358 346 144 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2124 2215 2394 513 Size: 552 1059 91 179 3907 Fragments arranged by size: 3907 1059 552 179 91 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5049 Fragments arranged by size: 5049 683 56 BtsI GCAGTG_nn' Cuts at: 2166 2478 3304 4098 4118 2166 Size: 312 826 794 20 3836 Fragments arranged by size: 3836 826 794 312 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5788 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5753 DraI TTT'AAA Cuts at: 3092 3092 Size: 5788 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3053 4795 809 Size: 507 1737 1742 1802 Fragments arranged by size: 1802 1742 1737 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2121 2292 2391 Size: 54 1425 50 275 18 291 171 99 Cuts at: 2391 2900 3235 4124 5753 8 Size: 509 335 889 1629 43 Fragments arranged by size: 1629 1425 889 509 335 291 275 171 99 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3235 1537 Size: 275 1423 4090 Fragments arranged by size: 4090 1423 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2252 2270 2351 2714 2795 2807 2915 3718 Size: 899 18 81 363 81 12 108 803 Cuts at: 3718 1353 Size: 3423 Fragments arranged by size: 3423 899 803 363 108 81 81 18 12 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4375 4749 4895 387 Size: 1064 138 2786 374 146 1280 Fragments arranged by size: 2786 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2075 2426 2631 3851 4448 5235 5503 Size: 393 443 351 205 1220 597 787 268 Cuts at: 5503 1239 Size: 1524 Fragments arranged by size: 1524 1220 787 597 443 393 351 268 205 EcoO109I rG'GnC_Cy Cuts at: 2324 2450 2324 Size: 126 5662 EcoRI G'AATT_C Cuts at: 3097 3475 3097 Size: 378 5410 FspI TGC'GCA Cuts at: 2048 4294 2048 Size: 2246 3542 HaeII r_GCGC'y Cuts at: 1678 2277 2486 2565 4935 1678 Size: 599 209 79 2370 2531 Fragments arranged by size: 2531 2370 599 209 79 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4429 4461 4503 4535 1373 Size: 32 135 32 2857 32 42 32 2626 Fragments arranged by size: 2857 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3132 3378 669 Size: 988 281 1194 246 3079 Fragments arranged by size: 3079 1194 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5788 HpaI GTT'AAC Cuts at: 3378 3378 Size: 5788 KasI G'GCGC_C Cuts at: 2561 2561 Size: 5788 KpnI G_GTAC'C Cuts at: 3113 3113 Size: 5788 MfeI C'AATT_G Cuts at: 3387 3387 Size: 5788 MluI A'CGCG_T Cuts at: 5664 5664 Size: 5788 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2115 4902 5086 1305 Size: 810 2787 184 2007 Fragments arranged by size: 2787 2007 810 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3966 Fragments arranged by size: 3966 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3746 4105 4264 518 Size: 648 2580 359 159 2042 Fragments arranged by size: 2580 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2030 2126 2217 2396 3204 3881 5029 5274 Size: 424 96 91 179 808 677 1148 245 Cuts at: 5274 1606 Size: 2120 Fragments arranged by size: 2120 1148 808 677 424 245 179 96 91 NarI GG'CG_CC Cuts at: 2562 2562 Size: 5788 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5236 NdeI CA'TA_TG Cuts at: 387 387 Size: 5788 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5788 NotI GC'GGCC_GC Cuts at: 3235 3235 Size: 5788 NspI r_CATG'y Cuts at: 1132 1229 3146 4691 1132 Size: 97 1917 1545 2229 Fragments arranged by size: 2229 1917 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5788 PciI A'CATG_T Cuts at: 4687 4687 Size: 5788 PflMI CCAn_nnn'nTGG Cuts at: 1108 2113 1108 Size: 1005 4783 PfoI T'CCnGG_A Cuts at: 2608 2608 Size: 5788 PmeI GTTT'AAAC Cuts at: 3092 3092 Size: 5788 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3857 3889 5370 5402 3857 Size: 32 1481 32 4243 Fragments arranged by size: 4243 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2450 2450 Size: 5788 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5788 PsiI TTA'TAA Cuts at: 3358 3358 Size: 5788 PspOMI G'GGCC_C Cuts at: 2323 2323 Size: 5788 PstI C_TGCA'G Cuts at: 830 2436 3140 830 Size: 1606 704 3478 Fragments arranged by size: 3478 1606 704 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5788 SacII CC_GC'GG Cuts at: 2127 2218 2397 2127 Size: 91 179 5518 Fragments arranged by size: 5518 179 91 SalI G'TCGA_C Cuts at: 1936 3130 1936 Size: 1194 4594 SapI GCTCTTCn'nnn_ Cuts at: 2270 2351 2795 2270 Size: 81 444 5263 Fragments arranged by size: 5263 444 81 SbfI CC_TGCA'GG Cuts at: 3140 3140 Size: 5788 ScaI AGT'ACT Cuts at: 4036 5748 4036 Size: 1712 4076 SexAI A'CCwGG_T Cuts at: 2889 2889 Size: 5788 SfcI C'TryA_G Cuts at: 826 1045 2432 3136 4271 4952 5143 826 Size: 219 1387 704 1135 681 191 1471 Fragments arranged by size: 1471 1387 1135 704 681 219 191 SfoI GGC'GCC Cuts at: 2563 2563 Size: 5788 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5788 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5788 SmaI CCC'GGG Cuts at: 1872 3115 1872 Size: 1243 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3892 4793 5055 820 Size: 197 926 1949 901 262 1553 Fragments arranged by size: 1949 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3579 493 Size: 3086 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5788 SphI G_CATG'C Cuts at: 1132 3146 1132 Size: 2014 3774 SspI AAT'ATT Cuts at: 5 52 3712 5 Size: 47 3660 2081 Fragments arranged by size: 3660 2081 47 StuI AGG'CCT Cuts at: 1517 2312 1517 Size: 795 4993 StyI C'CwwG_G Cuts at: 513 1065 3227 513 Size: 552 2162 3074 Fragments arranged by size: 3074 2162 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2908 3815 3832 3985 4170 4615 325 Size: 1255 1328 907 17 153 185 445 1498 Fragments arranged by size: 1498 1328 1255 907 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4034 5746 96 Size: 274 80 33 51 1443 2057 1712 138 Fragments arranged by size: 2057 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2062 2094 5748 5780 2062 Size: 32 3654 32 2070 Fragments arranged by size: 3654 2070 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2891 1316 Size: 1575 4213 XbaI T'CTAG_A Cuts at: 3124 3124 Size: 5788 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2386 2386 Size: 5788 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5788 XmaI C'CCGG_G Cuts at: 1870 3113 1870 Size: 1243 4545 XmnI GAAnn'nnTTC Cuts at: 3917 3917 Size: 5788 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2095 3596 276 Size: 53 83 186 936 561 1501 2468 Fragments arranged by size: 2468 1501 936 561 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacII SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AvrII BaeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspXI PsrI PvuII RsrII SacI SanDI SfiI SrfI SwaI