Restriction map for
pFN21AE9424
(Linear) (Six-base) MAPSORT of: KIEE9424.seq Check: 1110 from: 1 to: 1083
KIEE9424.orf
With 182 enzymes: SgfI *
November 2, 2010 17:11 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 344 434 1083
Size: 344 90 649
Fragments arranged by size:
649 344 90
AatII G_ACGT'C
Cuts at: 0 92 1083
Size: 92 991
AfeI AGC'GCT
Cuts at: 0 270 1083
Size: 270 813
AflIII A'CryG_T
Cuts at: 0 173 1083
Size: 173 910
ApaI G_GGCC'C
Cuts at: 0 322 1083
Size: 322 761
ApaLI G'TGCA_C
Cuts at: 0 421 1083
Size: 421 662
ApoI r'AATT_y
Cuts at: 0 569 686 995 1083
Size: 569 117 309 88
Fragments arranged by size:
569 309 117 88
AscI GG'CGCG_CC
Cuts at: 0 196 1083
Size: 196 887
BanI G'GyrC_C
Cuts at: 0 556 651 1083
Size: 556 95 432
Fragments arranged by size:
556 432 95
BanII G_rGCy'C
Cuts at: 0 322 835 1083
Size: 322 513 248
Fragments arranged by size:
513 322 248
BbeI G_GCGC'C
Cuts at: 0 560 1083
Size: 560 523
BbsI GAAGACnn'nnnn_
Cuts at: 0 516 1083
Size: 516 567
BclI T'GATC_A
Cuts at: 0 671 1083
Size: 671 412
BglII A'GATC_T
Cuts at: 0 611 1083
Size: 611 472
Bme1580I G_kGCm'C
Cuts at: 0 322 425 1083
Size: 322 103 658
Fragments arranged by size:
658 322 103
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 70 421 626 1083
Size: 70 351 205 457
Fragments arranged by size:
457 351 205 70
Bpu10I CC'TnA_GC
Cuts at: 0 358 1083
Size: 358 725
BsaI GGTCTCn'nnnn_
Cuts at: 0 816 1083
Size: 816 267
BsaAI yAC'GTr
Cuts at: 0 348 1083
Size: 348 735
BsaHI Gr'CG_yC
Cuts at: 0 89 207 557 1083
Size: 89 118 350 526
Fragments arranged by size:
526 350 118 89
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 793 844 1083
Size: 793 51 239
Fragments arranged by size:
793 239 51
BseYI C'CCAG_C
Cuts at: 0 165 315 767 1083
Size: 165 150 452 316
Fragments arranged by size:
452 316 165 150
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 996 1083
Size: 996 87
BsiEI CG_ry'CG
Cuts at: 0 503 1083
Size: 503 580
BsiHKAI G_wGCw'C
Cuts at: 0 146 425 1083
Size: 146 279 658
Fragments arranged by size:
658 279 146
BsmI GAATG_Cn'
Cuts at: 0 16 1083
Size: 16 1067
Bsp1286I G_dGCh'C
Cuts at: 0 146 322 425 835 1083
Size: 146 176 103 410 248
Fragments arranged by size:
410 248 176 146 103
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 263 344 434 1083
Size: 263 81 90 649
Fragments arranged by size:
649 263 90 81
BsrBI CCG'CTC
Cuts at: 0 362 508 1083
Size: 362 146 575
Fragments arranged by size:
575 362 146
BsrFI r'CCGG_y
Cuts at: 0 104 1083
Size: 104 979
BssHII G'CGCG_C
Cuts at: 0 14 196 1083
Size: 14 182 887
Fragments arranged by size:
887 182 14
BssSI C'ACGA_G
Cuts at: 0 615 1083
Size: 615 468
BstAPI GCAn_nnn'nTGC
Cuts at: 0 176 413 1083
Size: 176 237 670
Fragments arranged by size:
670 237 176
BstYI r'GATC_y
Cuts at: 0 611 755 1083
Size: 611 144 328
Fragments arranged by size:
611 328 144
BtgI C'CryG_G
Cuts at: 0 119 210 389 1083
Size: 119 91 179 694
Fragments arranged by size:
694 179 119 91
BtsI GCAGTG_nn'
Cuts at: 0 161 473 1083
Size: 161 312 610
Fragments arranged by size:
610 312 161
DrdI GACnn_nn'nnGTC
Cuts at: 0 1048 1083
Size: 1048 35
EaeI y'GGCC_r
Cuts at: 0 116 287 386 895 1083
Size: 116 171 99 509 188
Fragments arranged by size:
509 188 171 116 99
EarI CTCTTCn'nnn_
Cuts at: 0 247 265 346 709 790 802 910 1083
Size: 247 18 81 363 81 12 108 173
Fragments arranged by size:
363 247 173 108 81 81 18 12
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 70 421 626 1083
Size: 70 351 205 457
Fragments arranged by size:
457 351 205 70
EcoO109I rG'GnC_Cy
Cuts at: 0 319 445 1083
Size: 319 126 638
Fragments arranged by size:
638 319 126
FspI TGC'GCA
Cuts at: 0 43 1083
Size: 43 1040
HaeII r_GCGC'y
Cuts at: 0 272 481 560 1083
Size: 272 209 79 523
Fragments arranged by size:
523 272 209 79
KasI G'GCGC_C
Cuts at: 0 556 1083
Size: 556 527
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 110 1083
Size: 110 973
MspA1I CmG'CkG
Cuts at: 0 25 121 212 391 1083
Size: 25 96 91 179 692
Fragments arranged by size:
692 179 96 91 25
NarI GG'CG_CC
Cuts at: 0 557 1083
Size: 557 526
PflMI CCAn_nnn'nTGG
Cuts at: 0 108 1083
Size: 108 975
PfoI T'CCnGG_A
Cuts at: 0 603 1083
Size: 603 480
PpuMI rG'GwC_Cy
Cuts at: 0 445 1083
Size: 445 638
PspOMI G'GGCC_C
Cuts at: 0 318 1083
Size: 318 765
PstI C_TGCA'G
Cuts at: 0 431 1083
Size: 431 652
SacII CC_GC'GG
Cuts at: 0 122 213 392 1083
Size: 122 91 179 691
Fragments arranged by size:
691 179 122 91
SapI GCTCTTCn'nnn_
Cuts at: 0 265 346 790 1083
Size: 265 81 444 293
Fragments arranged by size:
444 293 265 81
SexAI A'CCwGG_T
Cuts at: 0 884 1083
Size: 884 199
SfcI C'TryA_G
Cuts at: 0 427 1083
Size: 427 656
SfoI GGC'GCC
Cuts at: 0 558 1083
Size: 558 525
StuI AGG'CCT
Cuts at: 0 307 1083
Size: 307 776
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 903 1083
Size: 903 180
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 57 89 1083
Size: 57 32 994
Fragments arranged by size:
994 57 32
Tth111I GACn'n_nGTC
Cuts at: 0 886 1083
Size: 886 197
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 381 1083
Size: 381 702
ZraI GAC'GTC
Cuts at: 0 90 1083
Size: 90 993
Enzymes that do cut:
AarI AatII AfeI AflIII ApaI ApaLI ApoI AscI
BanI BanII BbeI BbsI BclI BglII Bme1580I BpmI
Bpu10I BsaI BsaAI BsaHI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspMI BsrBI BsrFI BssHII BssSI
BstAPI BstYI BtgI BtsI DrdI EaeI EarI Eco57MI
EcoO109I FspI HaeII KasI MmeI MspA1I NarI PflMI
PfoI PpuMI PspOMI PstI SacII SapI SexAI SfcI
SfoI StuI TaqII TstI Tth111I XcmI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AcuI AflII AgeI AhdI AleI
AlfI AloI AlwNI AseI AsiSI AvaI AvrII BaeI
BamHI BbvCI BcgI BciVI BfrBI BglI BlpI BmgBI
BmrI BmtI BplI BpuEI BsaBI BsaWI BsaXI BsiWI
BsmBI BspEI BspHI BsrDI BsrGI BstBI BstEII BstXI
BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII EagI
EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspAI
Hin4I HincII HindIII HpaI KpnI MfeI MluI MscI
MslI NaeI NcoI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PasI PciI PmeI PmlI PpiI
PshAI PsiI PspXI PsrI PvuI PvuII RsrII SacI
SalI SanDI SbfI ScaI SfiI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StyI SwaI
TatI XbaI XhoI XmaI XmnI