(Circular) (Six-base) MAPSORT of: pFN21AE1841.seq Check: 7000 from: 1 to: 5776 pFN21AE1841 5776 bp With 182 enzymes: SgfI * August 16, 2010 14:49 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3586 278 Size: 53 83 186 936 2050 2468 Fragments arranged by size: 2468 2050 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2186 3030 3119 1937 Size: 249 844 89 4594 Fragments arranged by size: 4594 844 249 89 Acc65I G'GTAC_C Cuts at: 3097 3097 Size: 5776 AclI AA'CG_TT Cuts at: 1563 2782 3903 4276 1563 Size: 1219 1121 373 3063 Fragments arranged by size: 3063 1219 1121 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2898 3839 5223 5491 1632 Size: 1266 941 1384 268 1917 Fragments arranged by size: 1917 1384 1266 941 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5579 AflIII A'CryG_T Cuts at: 1203 2591 4675 5652 1203 Size: 1388 2084 977 1327 Fragments arranged by size: 2084 1388 1327 977 AgeI A'CCGG_T Cuts at: 4607 4607 Size: 5776 AhdI GACnn_n'nnGTC Cuts at: 4505 4505 Size: 5776 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5776 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5776 AlwNI CAG_nnn'CTG Cuts at: 3519 5091 3519 Size: 1572 4204 ApaI G_GGCC'C Cuts at: 2200 2200 Size: 5776 ApaLI G'TGCA_C Cuts at: 3833 4989 3833 Size: 1156 4620 ApoI r'AATT_y Cuts at: 1493 2621 2998 3085 3311 3463 5572 1493 Size: 1128 377 87 226 152 2109 1697 Fragments arranged by size: 2109 1697 1128 377 226 152 87 AseI AT'TA_AT Cuts at: 160 1032 2628 4330 160 Size: 872 1596 1702 1606 Fragments arranged by size: 1702 1606 1596 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5776 AvaI C'yCGr_G Cuts at: 1870 1943 3101 1870 Size: 73 1158 4545 Fragments arranged by size: 4545 1158 73 BamHI G'GATC_C Cuts at: 3106 3473 3106 Size: 367 5409 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3097 4552 618 Size: 325 217 627 1310 1455 1842 Fragments arranged by size: 1842 1455 1310 627 325 217 BanII G_rGCy'C Cuts at: 1391 2142 2200 3095 1391 Size: 751 58 895 4072 Fragments arranged by size: 4072 895 751 58 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4805 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3967 4001 1903 Size: 34 2030 34 3678 Fragments arranged by size: 3678 2030 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3668 4878 3668 Size: 1210 4566 BclI T'GATC_A Cuts at: 1548 1548 Size: 5776 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4387 136 Size: 107 122 71 631 605 2715 1525 Fragments arranged by size: 2715 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5771 5771 Size: 5776 BlpI GC'TnA_GC Cuts at: 3193 3193 Size: 5776 Bme1580I G_kGCm'C Cuts at: 1790 2200 3837 4993 1790 Size: 410 1637 1156 2573 Fragments arranged by size: 2573 1637 1156 410 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5776 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3507 4465 442 Size: 416 53 83 399 24 2090 958 1753 Fragments arranged by size: 2090 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5776 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4436 1239 Size: 3197 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3901 4766 5064 3901 Size: 865 298 4613 Fragments arranged by size: 4613 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2258 4439 882 Size: 617 759 2181 2219 Fragments arranged by size: 2219 2181 759 617 BsaAI yAC'GTr Cuts at: 493 1204 3567 493 Size: 711 2363 2702 Fragments arranged by size: 2702 2363 711 BsaBI GATnn'nnATC Cuts at: 5770 5770 Size: 5776 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3583 3965 4600 275 Size: 53 83 186 936 2050 382 635 1451 Fragments arranged by size: 2050 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2181 4209 4607 4881 5028 5439 2181 Size: 2028 398 274 147 411 2518 Fragments arranged by size: 2518 2028 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2969 2999 1178 Size: 30 165 30 1566 30 3955 Fragments arranged by size: 3955 1566 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5776 BseYI C'CCAG_C Cuts at: 2201 4979 5475 2201 Size: 2778 496 2502 Fragments arranged by size: 2778 2502 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5203 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3226 3987 5015 1540 Size: 275 186 1225 761 1028 2301 Fragments arranged by size: 2301 1225 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2489 3837 3922 4993 2489 Size: 1348 85 1071 3272 Fragments arranged by size: 3272 1348 1071 85 BsmI GAATG_Cn' Cuts at: 2283 3287 3380 2283 Size: 1004 93 4679 Fragments arranged by size: 4679 1004 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2142 2200 2489 3095 3837 3922 4993 Size: 399 352 58 289 606 742 85 1071 Cuts at: 4993 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 606 399 352 289 85 58 BspEI T'CCGG_A Cuts at: 5439 5439 Size: 5776 BspHI T'CATG_A Cuts at: 3665 5395 3665 Size: 1730 4046 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2874 3131 844 Size: 2030 257 3489 Fragments arranged by size: 3489 2030 257 BsrBI CCG'CTC Cuts at: 1394 1823 3663 1394 Size: 429 1840 3507 Fragments arranged by size: 3507 1840 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4271 4445 65 Size: 1183 3023 174 1396 Fragments arranged by size: 3023 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4420 4607 1700 Size: 110 2610 187 2869 Fragments arranged by size: 2869 2610 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5776 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5776 BssSI C'ACGA_G Cuts at: 3836 4848 3836 Size: 1012 4764 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5776 BstBI TT'CG_AA Cuts at: 2386 4580 2386 Size: 2194 3582 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5776 BstYI r'GATC_y Cuts at: 1972 2580 3106 3473 3858 3875 5327 5413 5425 Size: 608 526 367 385 17 1452 86 12 Cuts at: 5425 5771 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 608 526 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2121 2490 513 Size: 552 939 117 369 3799 Fragments arranged by size: 3799 939 552 369 117 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5037 Fragments arranged by size: 5037 683 56 BtsI GCAGTG_nn' Cuts at: 2615 3292 4086 4106 2615 Size: 677 794 20 4285 Fragments arranged by size: 4285 794 677 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5776 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5741 DraI TTT'AAA Cuts at: 2958 3080 2958 Size: 122 5654 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4783 809 Size: 507 3467 1802 Fragments arranged by size: 3467 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2041 2323 2944 Size: 54 1425 50 275 18 211 282 621 Cuts at: 2944 3223 4112 5741 8 Size: 279 889 1629 43 Fragments arranged by size: 1629 1425 889 621 282 279 275 211 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3223 1537 Size: 275 1411 4090 Fragments arranged by size: 4090 1411 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2094 2571 3706 1353 Size: 741 477 1135 3423 Fragments arranged by size: 3423 1135 741 477 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4363 4737 4883 387 Size: 1064 138 2774 374 146 1280 Fragments arranged by size: 2774 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2898 3839 4436 5223 5491 1239 Size: 393 1266 941 597 787 268 1524 Fragments arranged by size: 1524 1266 941 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2196 2197 2196 Size: 1 5775 EcoRI G'AATT_C Cuts at: 2621 2998 3085 3463 2621 Size: 377 87 378 4934 Fragments arranged by size: 4934 378 377 87 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2416 2416 Size: 5776 FspI TGC'GCA Cuts at: 4282 4282 Size: 5776 HaeII r_GCGC'y Cuts at: 1678 4923 1678 Size: 3245 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4417 4449 4491 4523 1373 Size: 32 135 32 2845 32 42 32 2626 Fragments arranged by size: 2845 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2506 3120 3366 669 Size: 988 281 568 614 246 3079 Fragments arranged by size: 3079 988 614 568 281 246 HindIII A'AGCT_T Cuts at: 748 2534 748 Size: 1786 3990 HpaI GTT'AAC Cuts at: 3366 3366 Size: 5776 KpnI G_GTAC'C Cuts at: 3101 3101 Size: 5776 MfeI C'AATT_G Cuts at: 3375 3375 Size: 5776 MluI A'CGCG_T Cuts at: 5652 5652 Size: 5776 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2206 2769 4890 5074 1305 Size: 901 563 2121 184 2007 Fragments arranged by size: 2121 2007 901 563 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2325 10 Size: 54 1425 343 493 3461 Fragments arranged by size: 3461 1425 493 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2334 3734 4093 4252 518 Size: 648 1168 1400 359 159 2042 Fragments arranged by size: 2042 1400 1168 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2903 3192 3869 5017 5262 1606 Size: 1297 289 677 1148 245 2120 Fragments arranged by size: 2120 1297 1148 677 289 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2121 2490 513 Size: 552 939 117 369 3799 Fragments arranged by size: 3799 939 552 369 117 NdeI CA'TA_TG Cuts at: 387 2730 387 Size: 2343 3433 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5776 NotI GC'GGCC_GC Cuts at: 3223 3223 Size: 5776 NspI r_CATG'y Cuts at: 1132 1229 2595 2837 3134 4679 1132 Size: 97 1366 242 297 1545 2229 Fragments arranged by size: 2229 1545 1366 297 242 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5776 PciI A'CATG_T Cuts at: 2591 4675 2591 Size: 2084 3692 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5776 PfoI T'CCnGG_A Cuts at: 2463 2463 Size: 5776 PmeI GTTT'AAAC Cuts at: 3080 3080 Size: 5776 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3845 3877 5358 5390 3845 Size: 32 1481 32 4231 Fragments arranged by size: 4231 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5776 PsiI TTA'TAA Cuts at: 2030 3346 2030 Size: 1316 4460 PspOMI G'GGCC_C Cuts at: 2196 2196 Size: 5776 PstI C_TGCA'G Cuts at: 830 3128 830 Size: 2298 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5776 SalI G'TCGA_C Cuts at: 1936 3118 1936 Size: 1182 4594 SbfI CC_TGCA'GG Cuts at: 3128 3128 Size: 5776 ScaI AGT'ACT Cuts at: 2475 4024 5736 2475 Size: 1549 1712 2515 Fragments arranged by size: 2515 1712 1549 SfcI C'TryA_G Cuts at: 826 1045 2603 2838 3124 4259 4940 5131 826 Size: 219 1558 235 286 1135 681 191 1471 Fragments arranged by size: 1558 1471 1135 681 286 235 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5776 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5776 SmaI CCC'GGG Cuts at: 1872 3103 1872 Size: 1231 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3880 4781 5043 820 Size: 197 926 1937 901 262 1553 Fragments arranged by size: 1937 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3567 493 Size: 3074 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5776 SphI G_CATG'C Cuts at: 1132 2837 3134 1132 Size: 1705 297 3774 Fragments arranged by size: 3774 1705 297 SspI AAT'ATT Cuts at: 5 52 2953 3700 5 Size: 47 2901 747 2081 Fragments arranged by size: 2901 2081 747 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5776 StyI C'CwwG_G Cuts at: 513 1065 2004 2121 2490 3215 513 Size: 552 939 117 369 725 3074 Fragments arranged by size: 3074 939 725 552 369 117 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3803 3820 3973 4158 4603 325 Size: 1255 2223 17 153 185 445 1498 Fragments arranged by size: 2223 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2473 4022 5734 Size: 274 80 33 51 1443 496 1549 1712 Cuts at: 5734 96 Size: 138 Fragments arranged by size: 1712 1549 1443 496 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2163 2195 2790 2822 5736 5768 2163 Size: 32 595 32 2914 32 2171 Fragments arranged by size: 2914 2171 595 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5776 XbaI T'CTAG_A Cuts at: 3112 3112 Size: 5776 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2333 2735 2333 Size: 402 5374 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5776 XmaI C'CCGG_G Cuts at: 1870 3101 1870 Size: 1231 4545 XmnI GAAnn'nnTTC Cuts at: 2784 3905 2784 Size: 1121 4655 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3584 276 Size: 53 83 186 936 2050 2468 Fragments arranged by size: 2468 2050 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI