################################## # NO CUT ENZYMES ( 94 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AflII AhdI Alw44I AocI ApaLI AscI AseI AsnI Asp718I AspEI AvrII BbeI BbrPI BbvCI BcgI BciVI BfrI BglII BlnI BsaAI BsaHI BseAI BsiEI BsiWI BsmBI Bsp1407I BspEI BspHI BspMI BsrBI BsrGI BssSI Bst1107I BstBI BstZ17I Bsu36I CpoI DraIII EagI Eam1105I EclXI Eco52I Eco81I EcoNI EcoT22I FseI HgaI Hin1I HpaI KasI KpnI KspI MluI MroI NarI NcoI NheI NotI NruI NsiI NspV PacI PaeR7I PmaCI PmeI PmlI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SnaBI Sse8387I SspBI SwaI XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 60 enzymes) # ################################### AccI 994 GTCTAC GT^[AC][GT]AC AflIII 435 ACATGT A^C[GA][CT]GT AgeI 654 ACCGGT A^CCGGT Aor51HI 146 AGCGCT AGC^GCT ApaI 2141 GGGCCC GGGCC^C Asp700I 1895 GAAGTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaI 584 CCCGAG C^[CT]CG[GA]G AviII 1113 TGCGCA TGC^GCA BalI 268 TGGCCA TGG^CCA BamHI 1797 GGATCC G^GATCC BclI 362 TGATCA T^GATCA BlpI 833 GCTTAGC GC^T[ACGT]AGC Bpu1102I 833 GCTTAGC GC^T[ACGT]AGC BsaBI 2725 GATCAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsgI 121 CTGCAC GTGCAG(16/14) BsoBI 584 CCCGAG C^[CT]CG[GA]G BspDI 2695 ATCGAT AT^CGAT BspLU11I 435 ACATGT A^CATGT BssHII 2097 GCGCGC G^CGCGC BstAPI 2113 GCAGCAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 1263 GGTTACC G^GT[ACGT]ACC BstPI 1263 GGTTACC G^GT[ACGT]ACC BstXI 1809 CCAGACAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 833 GCTTAGC GC^T[ACGT]AGC ClaI 2695 ATCGAT AT^CGAT DsaI 2155 CCACGG C^C[GA][CT]GG EarI 2641 CTCTTC CTCTTC(1/4) Eco47III 146 AGCGCT AGC^GCT EcoO65I 1263 GGTTACC G^GT[ACGT]ACC EcoRV 1327 GATATC GAT^ATC FauI 556 GCGGG CCCGC(4/6) FbaI 362 TGATCA T^GATCA FspI 1113 TGCGCA TGC^GCA HaeII 146 AGCGCT [GA]GCGC^[CT] HincII 2170 GTCAAC GT[CT]^[GA]AC HindII 2170 GTCAAC GT[CT]^[GA]AC HindIII 2815 AAGCTT A^AGCTT Ksp632I 2641 CTCTTC CTCTTC(1/4) MaeII 700 ACGT A^CGT MamI 2725 GATCAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 635 CAATTG C^AATTG MluNI 268 TGGCCA TGG^CCA MscI 268 TGGCCA TGG^CCA MslI 430 CATTCACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MunI 635 CAATTG C^AATTG NaeI 1303 GCCGGC GCC^GGC NdeI 2165 CATATG CA^TATG NgoMIV 1303 GCCGGC G^CCGGC PinAI 654 ACCGGT A^CCGGT PpuMI 1374 GGGACCC [GA]G^G[AT]CC[CT] PshAI 2244 GACGGAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 2102 CTGCAG CTGCA^G SacI 2638 GAGCTC GAGCT^C SapI 2640 GCTCTTC GCTCTTC(1/4) SexAI 542 ACCAGGT A^CC[AT]GGT SmlI 172 CTTGAG C^T[CT][GA]AG SpeI 781 ACTAGT A^CTAGT SphI 1886 GCATGC GCATG^C StuI 1344 AGGCCT AGG^CCT XmnI 1895 GAAGTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AccII 154 CGCG CG^CG 2098 CGCG AspI 100 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC 1361 GACTAGGTC BglI 1298 GCCCTGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1303 GCCGGCCTGGC BsaI 2450 GAGACC GGTCTC(1/5) 767 GGTCTC BsaWI 484 ACCGGA [AT]^CCGG[AT] 654 ACCGGT BseRI 1240 CTCCTC GAGGAG(10/8) 142 GAGGAG BsmI 1425 GAATGC GAATGC(1/-1) 1914 GAATGC BsrFI 654 ACCGGT [GA]^CCGG[CT] 1303 GCCGGC BstUI 154 CGCG CG^CG 2098 CGCG Cfr10I 654 ACCGGT [GA]^CCGG[CT] 1303 GCCGGC DraI 721 TTTAAA TTT^AAA 1707 TTTAAA DraII 1374 GGGACCC [GA]G^G[ACGT]CC[CT] 2140 AGGGCCC EcoO109I 1374 GGGACCC [GA]G^G[ACGT]CC[CT] 2140 AGGGCCC EcoRI 593 GAATTC G^AATTC 644 GAATTC HphI 1921 GGTGA GGTGA(8/7) 2241 GGTGA MvnI 154 CGCG CG^CG 2098 CGCG PflFI 100 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC 1361 GACTAGGTC PvuII 2117 CAGCTG CAG^CTG 2303 CAGCTG SfcI 551 CTATAG C^T[GA][CT]AG 2102 CTGCAG Tth111I 100 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC 1361 GACTAGGTC ######################################### # 3 OR MORE CUT ENZYMES ( 84 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI AlwNI ApoI AspHI AvaII BanI BanII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaJI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrDI BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DrdI EaeI EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII ItaI MaeI MaeIII MboI MboII MflI MnlI MseI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI RsaI Sau3AI Sau96I ScrFI SfaNI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII