(Circular) (Six-base) MAPSORT of: pFN21AE2540.seq Check: 5825 from: 1 to: 7627 pFN21AE2540 7627 bp With 182 enzymes: SgfI * September 19, 2014 18:12 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5437 278 Size: 53 83 186 936 3901 2468 Fragments arranged by size: 3901 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3000 4970 1937 Size: 1063 1970 4594 Fragments arranged by size: 4594 1970 1063 Acc65I G'GTAC_C Cuts at: 4948 4948 Size: 7627 AclI AA'CG_TT Cuts at: 1563 5754 6127 1563 Size: 4191 373 3063 Fragments arranged by size: 4191 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2223 2306 2429 3327 3879 4633 4691 4760 Size: 591 83 123 898 552 754 58 69 Cuts at: 4760 5690 7074 7342 1632 Size: 930 1384 268 1917 Fragments arranged by size: 1917 1384 930 898 754 591 552 268 123 83 69 58 AfeI AGC'GCT Cuts at: 2153 2153 Size: 7627 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7430 AflIII A'CryG_T Cuts at: 1203 2440 6526 7503 1203 Size: 1237 4086 977 1327 Fragments arranged by size: 4086 1327 1237 977 AgeI A'CCGG_T Cuts at: 2659 6458 2659 Size: 3799 3828 AhdI GACnn_n'nnGTC Cuts at: 6356 6356 Size: 7627 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7627 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7627 AlwNI CAG_nnn'CTG Cuts at: 4046 4124 4738 5370 6942 4046 Size: 78 614 632 1572 4731 Fragments arranged by size: 4731 1572 632 614 78 ApaI G_GGCC'C Cuts at: 4150 4150 Size: 7627 ApaLI G'TGCA_C Cuts at: 5684 6840 5684 Size: 1156 6471 ApoI r'AATT_y Cuts at: 1493 2598 2649 4490 4936 5162 5314 7423 1493 Size: 1105 51 1841 446 226 152 2109 1697 Fragments arranged by size: 2109 1841 1697 1105 446 226 152 51 AseI AT'TA_AT Cuts at: 160 1032 6181 160 Size: 872 5149 1606 Fragments arranged by size: 5149 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7627 AvaI C'yCGr_G Cuts at: 1870 1943 2589 4952 1870 Size: 73 646 2363 4545 Fragments arranged by size: 4545 2363 646 73 BamHI G'GATC_C Cuts at: 3802 4957 5324 3802 Size: 1155 367 6105 Fragments arranged by size: 6105 1155 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3011 3499 4766 4948 6403 Size: 325 217 627 1224 488 1267 182 1455 Cuts at: 6403 618 Size: 1842 Fragments arranged by size: 1842 1455 1267 1224 627 488 325 217 182 BanII G_rGCy'C Cuts at: 1391 2025 4150 4385 4647 4946 1391 Size: 634 2125 235 262 299 4072 Fragments arranged by size: 4072 2125 634 299 262 235 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2232 3050 3136 928 Size: 971 333 818 86 5419 Fragments arranged by size: 5419 971 818 333 86 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5818 5852 1903 Size: 34 3881 34 3678 Fragments arranged by size: 3881 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5519 6729 5519 Size: 1210 6417 BclI T'GATC_A Cuts at: 1548 2367 1548 Size: 819 6808 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3309 3314 6238 Size: 107 122 71 631 605 1637 5 2924 Cuts at: 6238 136 Size: 1525 Fragments arranged by size: 2924 1637 1525 631 605 122 107 71 5 BglII A'GATC_T Cuts at: 7622 7622 Size: 7627 BlpI GC'TnA_GC Cuts at: 2839 5044 2839 Size: 2205 5422 Bme1580I G_kGCm'C Cuts at: 1790 2091 3014 3502 3612 3624 4150 5688 6844 Size: 301 923 488 110 12 526 1538 1156 Cuts at: 6844 1790 Size: 2573 Fragments arranged by size: 2573 1538 1156 923 526 488 301 110 12 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7627 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2078 2967 4667 Size: 416 53 83 399 24 661 889 1700 Cuts at: 4667 5358 6316 442 Size: 691 958 1753 Fragments arranged by size: 1753 1700 958 889 691 661 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7627 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2990 3624 3717 4074 4297 4471 6287 1239 Size: 1751 634 93 357 223 174 1816 2579 Fragments arranged by size: 2579 1816 1751 634 357 223 174 93 Bpu10I CC'TnA_GC Cuts at: 4515 4641 4515 Size: 126 7501 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2198 5752 6617 6915 2198 Size: 3554 865 298 2910 Fragments arranged by size: 3554 2910 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2778 4449 6290 882 Size: 617 1279 1671 1841 2219 Fragments arranged by size: 2219 1841 1671 1279 617 BsaAI yAC'GTr Cuts at: 493 1204 5418 493 Size: 711 4214 2702 Fragments arranged by size: 4214 2702 711 BsaBI GATnn'nnATC Cuts at: 4734 7621 4734 Size: 2887 4740 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5434 5816 6451 275 Size: 53 83 186 936 3901 382 635 1451 Fragments arranged by size: 3901 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2489 2659 6060 6458 6732 6879 7290 2489 Size: 170 3401 398 274 147 411 2826 Fragments arranged by size: 3401 2826 411 398 274 170 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2039 2069 3765 3795 1178 Size: 30 165 30 636 30 1696 30 5010 Fragments arranged by size: 5010 1696 636 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2162 3236 1186 Size: 976 1074 5577 Fragments arranged by size: 5577 1074 976 BseYI C'CCAG_C Cuts at: 2831 3292 3323 3482 4128 4747 4923 6830 7326 Size: 461 31 159 646 619 176 1907 496 Cuts at: 7326 2831 Size: 3132 Fragments arranged by size: 3132 1907 646 619 496 461 176 159 31 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2111 1163 Size: 573 375 6679 Fragments arranged by size: 6679 573 375 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5077 5838 6866 1540 Size: 275 186 3076 761 1028 2301 Fragments arranged by size: 3076 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2864 2953 4422 4647 5688 5773 6844 2864 Size: 89 1469 225 1041 85 1071 3647 Fragments arranged by size: 3647 1469 1071 1041 225 89 85 BsmI GAATG_Cn' Cuts at: 3436 3925 5138 5231 3436 Size: 489 1213 93 5832 Fragments arranged by size: 5832 1213 489 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2025 2091 2864 2953 3014 3502 3612 Size: 399 235 66 773 89 61 488 110 Cuts at: 3612 3624 4150 4385 4422 4647 4946 5688 5773 Size: 12 526 235 37 225 299 742 85 Cuts at: 5773 6844 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 773 742 526 488 399 299 235 235 225 110 89 85 66 61 37 12 BspEI T'CCGG_A Cuts at: 7290 7290 Size: 7627 BspHI T'CATG_A Cuts at: 5516 7246 5516 Size: 1730 5897 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4982 844 Size: 4138 3489 BsrBI CCG'CTC Cuts at: 1394 1823 5514 1394 Size: 429 3691 3507 Fragments arranged by size: 3691 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3114 4603 4902 6122 6296 65 Size: 1183 1866 1489 299 1220 174 1396 Fragments arranged by size: 1866 1489 1396 1220 1183 299 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2659 3308 6271 6458 1700 Size: 110 849 649 2963 187 2869 Fragments arranged by size: 2963 2869 849 649 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7627 BssHII G'CGCG_C Cuts at: 1925 4102 1925 Size: 2177 5450 BssSI C'ACGA_G Cuts at: 5687 6699 5687 Size: 1012 6615 BstAPI GCAn_nnn'nTGC Cuts at: 794 4124 794 Size: 3330 4297 BstBI TT'CG_AA Cuts at: 6431 6431 Size: 7627 BstEII G'GTnAC_C Cuts at: 1184 3268 1184 Size: 2084 5543 BstXI CCAn_nnnn'nTGG Cuts at: 3821 3821 Size: 7627 BstYI r'GATC_y Cuts at: 1972 2311 2955 3103 3802 4735 4957 5324 5709 Size: 339 644 148 699 933 222 367 385 Cuts at: 5709 5726 7178 7264 7276 7622 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 933 699 644 385 367 346 339 222 148 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 4160 513 Size: 552 939 2156 3980 Fragments arranged by size: 3980 2156 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6888 Fragments arranged by size: 6888 683 56 BtsI GCAGTG_nn' Cuts at: 3268 5143 5937 5957 3268 Size: 1875 794 20 4938 Fragments arranged by size: 4938 1875 794 20 ClaI AT'CG_AT Cuts at: 1554 4701 1554 Size: 3147 4480 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7592 DraI TTT'AAA Cuts at: 2728 3714 4931 2728 Size: 986 1217 5424 Fragments arranged by size: 5424 1217 986 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2108 3823 4479 6634 809 Size: 507 792 1715 656 2155 1802 Fragments arranged by size: 2155 1802 1715 792 656 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2273 4918 Size: 54 1425 50 275 18 177 266 2645 Cuts at: 4918 5074 5963 7592 8 Size: 156 889 1629 43 Fragments arranged by size: 2645 1629 1425 889 275 266 177 156 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5074 1537 Size: 275 3262 4090 Fragments arranged by size: 4090 3262 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4652 5557 1353 Size: 3299 905 3423 Fragments arranged by size: 3423 3299 905 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6214 6588 6734 387 Size: 1064 138 4625 374 146 1280 Fragments arranged by size: 4625 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 4645 4645 Size: 7627 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2223 2306 2429 2990 3327 3624 3717 Size: 393 591 83 123 561 337 297 93 Cuts at: 3717 3879 4074 4297 4471 4633 4691 4760 5690 Size: 162 195 223 174 162 58 69 930 Cuts at: 5690 6287 7074 7342 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 930 787 597 591 561 393 337 297 268 223 195 174 162 162 123 93 83 69 58 EcoO109I rG'GnC_Cy Cuts at: 3380 4146 3380 Size: 766 6861 EcoRI G'AATT_C Cuts at: 2598 2649 4936 5314 2598 Size: 51 2287 378 4911 Fragments arranged by size: 4911 2287 378 51 EcoRV GAT'ATC Cuts at: 3334 3334 Size: 7627 FspI TGC'GCA Cuts at: 3120 6133 3120 Size: 3013 4614 HaeII r_GCGC'y Cuts at: 1678 2155 6774 1678 Size: 477 4619 2531 Fragments arranged by size: 4619 2531 477 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2039 2071 2484 2516 3778 Size: 32 135 32 467 32 413 32 1262 Cuts at: 3778 3810 3821 3853 3931 3963 4370 4402 6268 Size: 32 11 32 78 32 407 32 1866 Cuts at: 6268 6300 6342 6374 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1866 1262 467 413 407 135 78 42 32 32 32 32 32 32 32 32 32 32 11 HincII GTy'rAC Cuts at: 669 1657 1938 4177 4971 5217 669 Size: 988 281 2239 794 246 3079 Fragments arranged by size: 3079 2239 988 794 281 246 HindIII A'AGCT_T Cuts at: 748 4820 748 Size: 4072 3555 HpaI GTT'AAC Cuts at: 5217 5217 Size: 7627 KpnI G_GTAC'C Cuts at: 4952 4952 Size: 7627 MfeI C'AATT_G Cuts at: 2640 5226 2640 Size: 2586 5041 MluI A'CGCG_T Cuts at: 7503 7503 Size: 7627 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2025 2082 2541 4431 6741 6925 1305 Size: 720 57 459 1890 2310 184 2007 Fragments arranged by size: 2310 2007 1890 720 459 184 57 MscI TGG'CCA Cuts at: 10 64 1489 1832 2275 10 Size: 54 1425 343 443 5362 Fragments arranged by size: 5362 1425 443 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2439 5585 5944 6103 518 Size: 648 1273 3146 359 159 2042 Fragments arranged by size: 3146 2042 1273 648 359 159 MspA1I CmG'CkG Cuts at: 1606 4124 4310 4923 5043 5720 6868 7113 1606 Size: 2518 186 613 120 677 1148 245 2120 Fragments arranged by size: 2518 2120 1148 677 613 245 186 120 NaeI GCC'GGC Cuts at: 3310 3310 Size: 7627 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 6136 Fragments arranged by size: 6136 939 552 NdeI CA'TA_TG Cuts at: 387 4171 387 Size: 3784 3843 NgoMIV G'CCGG_C Cuts at: 3308 3308 Size: 7627 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7627 NotI GC'GGCC_GC Cuts at: 5074 5074 Size: 7627 NspI r_CATG'y Cuts at: 1132 1229 2444 3321 3725 3895 4183 4985 6530 Size: 97 1215 877 404 170 288 802 1545 Cuts at: 6530 1132 Size: 2229 Fragments arranged by size: 2229 1545 1215 877 802 404 288 170 97 PasI CC'CwG_GG Cuts at: 1348 2014 1348 Size: 666 6961 PciI A'CATG_T Cuts at: 2440 6526 2440 Size: 4086 3541 PflMI CCAn_nnn'nTGG Cuts at: 1108 2645 3473 3495 1108 Size: 1537 828 22 5240 Fragments arranged by size: 5240 1537 828 22 PfoI T'CCnGG_A Cuts at: 4854 4854 Size: 7627 PmeI GTTT'AAAC Cuts at: 4931 4931 Size: 7627 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2935 2967 4533 4565 5696 5728 7209 7241 2935 Size: 32 1566 32 1131 32 1481 32 3321 Fragments arranged by size: 3321 1566 1481 1131 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3380 3380 Size: 7627 PshAI GACnn'nnGTC Cuts at: 1613 4253 1613 Size: 2640 4987 PsiI TTA'TAA Cuts at: 5197 5197 Size: 7627 PspOMI G'GGCC_C Cuts at: 4146 4146 Size: 7627 PstI C_TGCA'G Cuts at: 830 4111 4979 830 Size: 3281 868 3478 Fragments arranged by size: 3478 3281 868 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7627 PvuII CAG'CTG Cuts at: 4124 4310 4124 Size: 186 7441 SacI G_AGCT'C Cuts at: 4647 4647 Size: 7627 SalI G'TCGA_C Cuts at: 1936 4969 1936 Size: 3033 4594 SanDI GG'GwC_CC Cuts at: 3380 3380 Size: 7627 SapI GCTCTTCn'nnn_ Cuts at: 4652 4652 Size: 7627 SbfI CC_TGCA'GG Cuts at: 4979 4979 Size: 7627 ScaI AGT'ACT Cuts at: 5875 7587 5875 Size: 1712 5915 SexAI A'CCwGG_T Cuts at: 2547 2547 Size: 7627 SfcI C'TryA_G Cuts at: 826 1045 2556 4107 4975 6110 6791 6982 826 Size: 219 1511 1551 868 1135 681 191 1471 Fragments arranged by size: 1551 1511 1471 1135 868 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7627 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7627 SmaI CCC'GGG Cuts at: 1872 4954 1872 Size: 3082 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2177 5731 6632 6894 820 Size: 197 926 234 3554 901 262 1553 Fragments arranged by size: 3554 1553 926 901 262 234 197 SnaBI TAC'GTA Cuts at: 493 5418 493 Size: 4925 2702 SpeI A'CTAG_T Cuts at: 152 2786 152 Size: 2634 4993 SphI G_CATG'C Cuts at: 1132 3895 4985 1132 Size: 2763 1090 3774 Fragments arranged by size: 3774 2763 1090 SspI AAT'ATT Cuts at: 5 52 2467 2713 3592 5551 5 Size: 47 2415 246 879 1959 2081 Fragments arranged by size: 2415 2081 1959 879 246 47 StuI AGG'CCT Cuts at: 1517 3351 1517 Size: 1834 5793 StyI C'CwwG_G Cuts at: 513 1065 2004 2358 3352 3954 5066 513 Size: 552 939 354 994 602 1112 3074 Fragments arranged by size: 3074 1112 994 939 602 552 354 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3496 3701 4473 5654 5671 5824 6009 Size: 1255 1916 205 772 1181 17 153 185 Cuts at: 6009 6454 325 Size: 445 1498 Fragments arranged by size: 1916 1498 1255 1181 772 445 205 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 5873 7585 96 Size: 274 80 33 51 1443 3896 1712 138 Fragments arranged by size: 3896 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3765 3797 3910 3942 7587 7619 3765 Size: 32 113 32 3645 32 3773 Fragments arranged by size: 3773 3645 113 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2108 3369 1316 Size: 792 1261 5574 Fragments arranged by size: 5574 1261 792 XbaI T'CTAG_A Cuts at: 4963 4963 Size: 7627 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7627 XmaI C'CCGG_G Cuts at: 1870 4952 1870 Size: 3082 4545 XmnI GAAnn'nnTTC Cuts at: 3904 5756 3904 Size: 1852 5775 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5435 276 Size: 53 83 186 936 3901 2468 Fragments arranged by size: 3901 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SfiI SfoI SrfI SwaI XcmI