(Circular) (Six-base) MAPSORT of: pFN21AE5930.seq Check: 1411 from: 1 to: 5638 pFN21AE5930 5638 bp With 182 enzymes: SgfI * December 8, 2014 13:47 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2059 2726 2059 Size: 667 4971 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3448 278 Size: 53 83 186 936 1912 2468 Fragments arranged by size: 2468 1912 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2981 1937 Size: 1044 4594 Acc65I G'GTAC_C Cuts at: 2959 2959 Size: 5638 AclI AA'CG_TT Cuts at: 1563 3765 4138 1563 Size: 2202 373 3063 Fragments arranged by size: 3063 2202 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2207 2670 3701 5085 5353 1632 Size: 575 463 1031 1384 268 1917 Fragments arranged by size: 1917 1384 1031 575 463 268 AfeI AGC'GCT Cuts at: 2112 2112 Size: 5638 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5441 AflIII A'CryG_T Cuts at: 1203 2484 4537 5514 1203 Size: 1281 2053 977 1327 Fragments arranged by size: 2053 1327 1281 977 AgeI A'CCGG_T Cuts at: 4469 4469 Size: 5638 AhdI GACnn_n'nnGTC Cuts at: 4367 4367 Size: 5638 AleI CACnn'nnGTG Cuts at: 1166 2021 1166 Size: 855 4783 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5638 AlwNI CAG_nnn'CTG Cuts at: 2074 2228 3381 4953 2074 Size: 154 1153 1572 2759 Fragments arranged by size: 2759 1572 1153 154 ApaLI G'TGCA_C Cuts at: 3695 4851 3695 Size: 1156 4482 ApoI r'AATT_y Cuts at: 1493 2030 2947 3173 3325 5434 1493 Size: 537 917 226 152 2109 1697 Fragments arranged by size: 2109 1697 917 537 226 152 AseI AT'TA_AT Cuts at: 160 1032 4192 160 Size: 872 3160 1606 Fragments arranged by size: 3160 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5638 AvaI C'yCGr_G Cuts at: 1870 1943 2963 1870 Size: 73 1020 4545 Fragments arranged by size: 4545 1020 73 BamHI G'GATC_C Cuts at: 2968 3335 2968 Size: 367 5271 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2959 4414 618 Size: 325 217 627 1172 1455 1842 Fragments arranged by size: 1842 1455 1172 627 325 217 BanII G_rGCy'C Cuts at: 1391 2609 2766 2957 1391 Size: 1218 157 191 4072 Fragments arranged by size: 4072 1218 191 157 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4667 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3829 3863 1903 Size: 34 1892 34 3678 Fragments arranged by size: 3678 1892 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3530 4740 3530 Size: 1210 4428 BclI T'GATC_A Cuts at: 1548 1548 Size: 5638 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4249 136 Size: 107 122 71 631 605 2577 1525 Fragments arranged by size: 2577 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5633 5633 Size: 5638 BlpI GC'TnA_GC Cuts at: 2328 2447 3055 2328 Size: 119 608 4911 Fragments arranged by size: 4911 608 119 Bme1580I G_kGCm'C Cuts at: 1790 3699 4855 1790 Size: 1909 1156 2573 Fragments arranged by size: 2573 1909 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5638 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3369 4327 442 Size: 416 53 83 399 24 1952 958 1753 Fragments arranged by size: 1952 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5638 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4298 1239 Size: 3059 2579 Bpu10I CC'TnA_GC Cuts at: 2603 2603 Size: 5638 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2280 3763 4628 4926 2280 Size: 1483 865 298 2992 Fragments arranged by size: 2992 1483 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4301 882 Size: 617 2802 2219 Fragments arranged by size: 2802 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3429 493 Size: 711 2225 2702 Fragments arranged by size: 2702 2225 711 BsaBI GATnn'nnATC Cuts at: 5632 5632 Size: 5638 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3445 3827 4462 275 Size: 53 83 186 936 1912 382 635 1451 Fragments arranged by size: 1912 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4071 4469 4743 4890 5301 4071 Size: 398 274 147 411 4408 Fragments arranged by size: 4408 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5413 Fragments arranged by size: 5413 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5638 BseYI C'CCAG_C Cuts at: 2727 4841 5337 2727 Size: 2114 496 3028 Fragments arranged by size: 3028 2114 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2060 1163 Size: 573 324 4741 Fragments arranged by size: 4741 573 324 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3088 3849 4877 1540 Size: 275 186 1087 761 1028 2301 Fragments arranged by size: 2301 1087 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2499 2609 2676 3699 3784 4855 2499 Size: 110 67 1023 85 1071 3282 Fragments arranged by size: 3282 1071 1023 110 85 67 BsmI GAATG_Cn' Cuts at: 3149 3242 3149 Size: 93 5545 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2499 2609 2676 2766 2957 3699 3784 Size: 399 709 110 67 90 191 742 85 Cuts at: 3784 4855 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 709 399 191 110 90 85 67 BspEI T'CCGG_A Cuts at: 5301 5301 Size: 5638 BspHI T'CATG_A Cuts at: 2637 3527 5257 2637 Size: 890 1730 3018 Fragments arranged by size: 3018 1730 890 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2059 2133 2726 2993 844 Size: 1215 74 593 267 3489 Fragments arranged by size: 3489 1215 593 267 74 BsrBI CCG'CTC Cuts at: 1394 1823 3525 1394 Size: 429 1702 3507 Fragments arranged by size: 3507 1702 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2133 4133 4307 65 Size: 1183 885 2000 174 1396 Fragments arranged by size: 2000 1396 1183 885 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4282 4469 1700 Size: 110 2472 187 2869 Fragments arranged by size: 2869 2472 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5638 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5638 BssSI C'ACGA_G Cuts at: 3698 4710 3698 Size: 1012 4626 BstAPI GCAn_nnn'nTGC Cuts at: 794 2074 794 Size: 1280 4358 BstBI TT'CG_AA Cuts at: 4442 4442 Size: 5638 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5638 BstYI r'GATC_y Cuts at: 1972 2206 2507 2968 3335 3720 3737 5189 5275 Size: 234 301 461 367 385 17 1452 86 Cuts at: 5275 5287 5633 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 461 385 367 346 301 234 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2776 2776 Size: 5638 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4147 Fragments arranged by size: 4147 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4899 Fragments arranged by size: 4899 683 56 BtsI GCAGTG_nn' Cuts at: 3154 3948 3968 3154 Size: 794 20 4824 Fragments arranged by size: 4824 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5638 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5603 DraI TTT'AAA Cuts at: 2942 2942 Size: 5638 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4645 809 Size: 507 3329 1802 Fragments arranged by size: 3329 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3085 3974 5603 Size: 54 1425 50 275 18 1255 889 1629 Cuts at: 5603 8 Size: 43 Fragments arranged by size: 1629 1425 1255 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3085 1537 Size: 275 1273 4090 Fragments arranged by size: 4090 1273 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3568 1353 Size: 2215 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4225 4599 4745 387 Size: 1064 138 2636 374 146 1280 Fragments arranged by size: 2636 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2607 2607 Size: 5638 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2207 2670 3701 4298 5085 5353 1239 Size: 393 575 463 1031 597 787 268 1524 Fragments arranged by size: 1524 1031 787 597 575 463 393 268 EcoRI G'AATT_C Cuts at: 2030 2947 3325 2030 Size: 917 378 4343 Fragments arranged by size: 4343 917 378 FspI TGC'GCA Cuts at: 4144 4144 Size: 5638 HaeII r_GCGC'y Cuts at: 1678 2114 4785 1678 Size: 436 2671 2531 Fragments arranged by size: 2671 2531 436 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4279 4311 4353 4385 1373 Size: 32 135 32 2707 32 42 32 2626 Fragments arranged by size: 2707 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2982 3228 669 Size: 988 281 1044 246 3079 Fragments arranged by size: 3079 1044 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5638 HpaI GTT'AAC Cuts at: 3228 3228 Size: 5638 KpnI G_GTAC'C Cuts at: 2963 2963 Size: 5638 MfeI C'AATT_G Cuts at: 2784 3237 2784 Size: 453 5185 MluI A'CGCG_T Cuts at: 5514 5514 Size: 5638 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4752 4936 1305 Size: 3447 184 2007 Fragments arranged by size: 3447 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3816 Fragments arranged by size: 3816 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2021 2171 3596 3955 4114 518 Size: 648 855 150 1425 359 159 2042 Fragments arranged by size: 2042 1425 855 648 359 159 150 MspA1I CmG'CkG Cuts at: 1606 3054 3731 4879 5124 1606 Size: 1448 677 1148 245 2120 Fragments arranged by size: 2120 1448 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4147 Fragments arranged by size: 4147 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5638 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5638 NotI GC'GGCC_GC Cuts at: 3085 3085 Size: 5638 NspI r_CATG'y Cuts at: 1132 1229 2488 2996 4541 1132 Size: 97 1259 508 1545 2229 Fragments arranged by size: 2229 1545 1259 508 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5638 PciI A'CATG_T Cuts at: 2484 4537 2484 Size: 2053 3585 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5638 PmeI GTTT'AAAC Cuts at: 2942 2942 Size: 5638 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3707 3739 5220 5252 3707 Size: 32 1481 32 4093 Fragments arranged by size: 4093 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5638 PsiI TTA'TAA Cuts at: 3208 3208 Size: 5638 PstI C_TGCA'G Cuts at: 830 2070 2990 830 Size: 1240 920 3478 Fragments arranged by size: 3478 1240 920 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5638 SacI G_AGCT'C Cuts at: 2609 2609 Size: 5638 SalI G'TCGA_C Cuts at: 1936 2980 1936 Size: 1044 4594 SbfI CC_TGCA'GG Cuts at: 2990 2990 Size: 5638 ScaI AGT'ACT Cuts at: 3886 5598 3886 Size: 1712 3926 SfcI C'TryA_G Cuts at: 826 1045 2066 2986 4121 4802 4993 826 Size: 219 1021 920 1135 681 191 1471 Fragments arranged by size: 1471 1135 1021 920 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5638 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5638 SmaI CCC'GGG Cuts at: 1872 2965 1872 Size: 1093 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2295 3742 4643 4905 820 Size: 197 926 352 1447 901 262 1553 Fragments arranged by size: 1553 1447 926 901 352 262 197 SnaBI TAC'GTA Cuts at: 493 3429 493 Size: 2936 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5638 SphI G_CATG'C Cuts at: 1132 2996 1132 Size: 1864 3774 SspI AAT'ATT Cuts at: 5 52 3562 5 Size: 47 3510 2081 Fragments arranged by size: 3510 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5638 StyI C'CwwG_G Cuts at: 513 1065 2004 3077 513 Size: 552 939 1073 3074 Fragments arranged by size: 3074 1073 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2185 3665 3682 3835 4020 4465 325 Size: 1255 605 1480 17 153 185 445 1498 Fragments arranged by size: 1498 1480 1255 605 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2175 3884 5596 Size: 274 80 33 51 1443 198 1709 1712 Cuts at: 5596 96 Size: 138 Fragments arranged by size: 1712 1709 1443 274 198 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5598 5630 5598 Size: 32 5606 Tth111I GACn'n_nGTC Cuts at: 1316 2694 1316 Size: 1378 4260 XbaI T'CTAG_A Cuts at: 2974 2974 Size: 5638 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5638 XmaI C'CCGG_G Cuts at: 1870 2963 1870 Size: 1093 4545 XmnI GAAnn'nnTTC Cuts at: 2683 3767 2683 Size: 1084 4554 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3446 276 Size: 53 83 186 936 1912 2468 Fragments arranged by size: 2468 1912 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I DraIII EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI