################################## # NO CUT ENZYMES ( 149 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwI Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaII AviII BamHI BbeI BbrPI BbsI BbvCI BciVI BclI BcnI BfrI BglI BpmI BpuAI BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmBI BsmI BspEI BspLU11I BsrBI BsrDI BsrFI BsrI BssHII BssSI Bst1107I BstBI BstUI BstZ17I CfoI Cfr10I CpoI DraI DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I EcoRV EcoT22I FbaI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HindIII HpaI HpaII KasI KpnI KspI MamI MfeI MluI MroI MseI MspA1I MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PstI PvuI PvuII RsrII SacI SacII SapI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SmlI SpeI SphI Sse8387I SspI StuI SwaI TfiI Tru9I Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### AccI 247 GTCGAC GT^[AC][GT]AC AcsI 659 GAATTC [GA]^AATT[CT] AlwNI 323 CAGGGGCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 896 GGGCCC GGGCC^C ApoI 659 GAATTC [GA]^AATT[CT] AvrII 342 CCTAGG C^CTAGG BalI 713 TGGCCA TGG^CCA BanI 540 GGTGCC G^G[CT][GA]CC BcgI 537 CGAGGTGCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfaI 343 CTAG C^TAG BglII 416 AGATCT A^GATCT BlnI 342 CCTAGG C^CTAGG BlpI 186 GCTCAGC GC^T[ACGT]AGC Bpu1102I 186 GCTCAGC GC^T[ACGT]AGC BsmAI 586 GAGAC GTCTC(1/5) BsmFI 206 GGGAC GGGAC(10/14) Bsp1407I 767 TGTACA T^GTACA BspDI 460 ATCGAT AT^CGAT BspHI 404 TCATGA T^CATGA BspMI 718 ACCTGC ACCTGC(4/8) BsrGI 767 TGTACA T^GTACA BstAPI 413 GCAAGATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 939 GGTAACC G^GT[ACGT]ACC BstPI 939 GGTAACC G^GT[ACGT]ACC BstYI 416 AGATCT [GA]^GATC[CT] CelII 186 GCTCAGC GC^T[ACGT]AGC ClaI 460 ATCGAT AT^CGAT DraIII 730 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG EaeI 713 TGGCCA [CT]^GGCC[GA] EarI 748 CTCTTC CTCTTC(1/4) EcoNI 900 CCTACAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 939 GGTAACC G^GT[ACGT]ACC EcoRI 659 GAATTC G^AATTC EcoT14I 342 CCTAGG C^C[AT][AT]GG FauI 158 CCCGC CCCGC(4/6) HincII 247 GTCGAC GT[CT]^[GA]AC HindII 247 GTCGAC GT[CT]^[GA]AC Ksp632I 748 CTCTTC CTCTTC(1/4) MaeI 343 CTAG C^TAG MflI 416 AGATCT [GA]^GATC[CT] MluNI 713 TGGCCA TGG^CCA MscI 713 TGGCCA TGG^CCA PshAI 347 GACTCATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 404 TCATGA T^CATGA SalI 247 GTCGAC G^TCGAC SexAI 251 ACCAGGT A^CC[AT]GGT SfcI 901 CTACAG C^T[GA][CT]AG SnaBI 367 TACGTA TAC^GTA SspBI 767 TGTACA T^GTACA StyI 342 CCTAGG C^C[AT][AT]GG TspRI 291 CACTG CA[GC]TG[ACGT][ACGT]^ XhoII 416 AGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AfaI 177 GTAC GT^AC 768 GTAC AvaI 512 CTCGGG C^[CT]CG[GA]G 642 CTCGGG BanII 645 GGGCTC G[GA]GC[CT]^C 896 GGGCCC BsaAI 367 TACGTA [CT]AC^GT[GA] 775 TACGTG BsaJI 70 CCCTGG C^C[ACGT][ACGT]GG 342 CCTAGG BseRI 619 CTCCTC GAGGAG(10/8) 723 CTCCTC BsgI 388 CTGCAC GTGCAG(16/14) 564 CTGCAC BsoBI 512 CTCGGG C^[CT]CG[GA]G 642 CTCGGG BstXI 119 CCAGCAACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 236 CCAAAATGCTGG Cfr13I 896 GGGCC G^G[ACGT]CC 897 GGCCC DraII 895 GGGGCCC [GA]G^G[ACGT]CC[CT] 896 GGGCCCT EcoO109I 895 GGGGCCC [GA]G^G[ACGT]CC[CT] 896 GGGCCCT HaeIII 714 GGCC GG^CC 897 GGCC HinfI 154 GACTC G^A[ACGT]TC 347 GACTC PleI 154 GACTC GAGTC(4/5) 347 GACTC RsaI 177 GTAC GT^AC 768 GTAC Sau96I 896 GGGCC G^G[ACGT]CC 897 GGCCC SfaNI 287 GATGC GCATC(5/9) 615 GATGC TaqI 248 TCGA T^CGA 461 TCGA Tsp509I 13 AATT ^AATT 660 AATT TthHB8I 248 TCGA T^CGA 461 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 36 enzymes) # ######################################### AciI AluI AocI BbvI BmyI BsiYI BslI Bsp1286I BssKI BstF5I BstNI Bsu36I Cac8I DdeI DpnII Eco81I EcoRII Fnu4HI FokI HphI ItaI MaeII MaeIII MboI MboII MnlI MslI MvaI MwoI NdeII NlaIII NlaIV Sau3AI ScrFI TseI Tsp45I