Restriction map for
pFN21AE6033
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# NO CUT ENZYMES ( 151 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaI AviII AvrII BamHI BbeI
BbrPI BbsI BcgI BclI BfrI
BglII BlnI BpuAI BsaBI BsaHI
BsaI BsaWI BseAI BseRI BsiEI
BsiWI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BsrGI BssHII Bst1107I BstAPI
BstBI BstF5I BstUI BstZ17I Bsu36I
ClaI CpoI DraIII EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoRI EcoRV EcoT22I FbaI
FokI FseI FspI HaeII Hin1I
HincII HindII HindIII HpaI KasI
KpnI Ksp632I KspI MamI MfeI
MluI MroI MunI MvnI NarI
NdeI NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SfaNI SfcI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SphI Sse8387I SspBI SspI
SwaI TaqI TfiI Tth111I TthHB8I
Van91I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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BanI 540 GGTGCC G^G[CT][GA]CC
BanII 321 GGGCTC G[GA]GC[CT]^C
BciVI 570 GGATAC GTATCC(6/5)
BcnI 627 CCCGG CC^[GC]GG
BglI 346 GCCGTCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 447 GCTCAGC GC^T[ACGT]AGC
BpmI 156 CTCCAG CTGGAG(16/14)
Bpu1102I 447 GCTCAGC GC^T[ACGT]AGC
BsaAI 304 TACGTG [CT]AC^GT[GA]
BsgI 490 GTGCAG GTGCAG(16/14)
BssSI 198 CTCGTG CACGAG(-5/-1)
BstEII 246 GGTTACC G^GT[ACGT]ACC
BstPI 246 GGTTACC G^GT[ACGT]ACC
BstXI 311 CCATGGGGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 796 AGATCC [GA]^GATC[CT]
CelII 447 GCTCAGC GC^T[ACGT]AGC
CfoI 390 GCGC GCG^C
DraI 852 TTTAAA TTT^AAA
DrdI 717 GACCTGTTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 311 CCATGG C^C[GA][CT]GG
EcoO65I 246 GGTTACC G^GT[ACGT]ACC
EcoT14I 311 CCATGG C^C[AT][AT]GG
FauI 694 GCGGG CCCGC(4/6)
HgaI 424 GCGTC GACGC(5/10)
HhaI 390 GCGC GCG^C
HinP1I 390 GCGC G^CGC
MflI 796 AGATCC [GA]^GATC[CT]
NaeI 380 GCCGGC GCC^GGC
NciI 627 CCCGG CC^[GC]GG
NcoI 311 CCATGG C^CATGG
NgoMIV 380 GCCGGC G^CCGGC
SexAI 731 ACCTGGT A^CC[AT]GGT
SpeI 138 ACTAGT A^CTAGT
StuI 707 AGGCCT AGG^CCT
StyI 311 CCATGG C^C[AT][AT]GG
Tsp45I 841 GTCAC ^GT[GC]AC
XcmI 702 CCAAAAGGCCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 796 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AlwI 797 GATCC GGATC(4/5)
522 GGATC
AlwNI 238 CAGTTGCTG CAG[ACGT][ACGT][ACGT]^CTG
414 CAGGTTCTG
AspHI 477 GTGCTC G[AT]GC[AT]^C
547 GTGCTC
BalI 308 TGGCCA TGG^CCA
602 TGGCCA
BbvCI 61 CCTCAGC CCTCAGC(-5/-2)
186 CCTCAGC
BfaI 67 CTAG C^TAG
139 CTAG
BsiHKAI 477 GTGCTC G[AT]GC[AT]^C
547 GTGCTC
BsmBI 425 CGTCTC CGTCTC(1/5)
725 CGTCTC
Cac8I 380 GCCGGC GC[ACGT]^[ACGT]GC
694 GCGGGC
HinfI 154 GACTC G^A[ACGT]TC
161 GACTC
MaeI 67 CTAG C^TAG
139 CTAG
MaeII 104 ACGT A^CGT
305 ACGT
MboII 36 TCTTC GAAGA(8/7)
182 TCTTC
MluNI 308 TGGCCA TGG^CCA
602 TGGCCA
MscI 308 TGGCCA TGG^CCA
602 TGGCCA
MseI 676 TTAA T^TAA
853 TTAA
MslI 168 CACTCCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
765 CATATTCATG
PleI 154 GACTC GAGTC(4/5)
161 GACTC
PpuMI 715 GGGACCT [GA]G^G[AT]CC[CT]
904 AGGACCC
PvuII 493 CAGCTG CAG^CTG
689 CAGCTG
Tru9I 676 TTAA T^TAA
853 TTAA
Tsp509I 471 AATT ^AATT
621 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AciI AfaI AluI AvaII BbvI
BmyI BsaJI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrFI BsrI BssKI
BstNI Cfr10I Cfr13I DdeI DpnII
DraII EaeI EcoO109I EcoRII Fnu4HI
HaeIII HapII HpaII HphI ItaI
MaeIII MboI MnlI MspA1I MspI
MvaI MwoI NdeII NlaIII NlaIV
RsaI Sau3AI Sau96I ScrFI SmlI
TseI TspRI