(Linear) (Six-base) MAPSORT of: KIEE6050.seq Check: 7866 from: 1 to: 954 KIEE6050.orf With 182 enzymes: SgfI * December 5, 2014 13:21 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 742 954 Size: 742 212 AatII G_ACGT'C Cuts at: 0 590 954 Size: 590 364 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 589 954 Size: 589 365 AleI CACnn'nnGTG Cuts at: 0 862 954 Size: 862 92 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 646 954 Size: 646 308 AlwNI CAG_nnn'CTG Cuts at: 0 444 890 954 Size: 444 446 64 Fragments arranged by size: 446 444 64 ApoI r'AATT_y Cuts at: 0 699 954 Size: 699 255 AvaI C'yCGr_G Cuts at: 0 798 954 Size: 798 156 BamHI G'GATC_C Cuts at: 0 308 954 Size: 308 646 BanII G_rGCy'C Cuts at: 0 173 915 954 Size: 173 742 39 Fragments arranged by size: 742 173 39 BbvCI CC'TCA_GC Cuts at: 0 344 954 Size: 344 610 BclI T'GATC_A Cuts at: 0 749 954 Size: 749 205 BglI GCCn_nnn'nGGC Cuts at: 0 460 954 Size: 460 494 BlpI GC'TnA_GC Cuts at: 0 818 954 Size: 818 136 Bme1580I G_kGCm'C Cuts at: 0 251 954 Size: 251 703 BmrI ACTGGGnnnn_n' Cuts at: 0 143 954 Size: 143 811 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 296 359 642 954 Size: 296 63 283 312 Fragments arranged by size: 312 296 283 63 Bpu10I CC'TnA_GC Cuts at: 0 6 344 954 Size: 6 338 610 Fragments arranged by size: 610 338 6 BsaAI yAC'GTr Cuts at: 0 611 954 Size: 611 343 BsaHI Gr'CG_yC Cuts at: 0 587 954 Size: 587 367 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 332 362 954 Size: 332 30 592 Fragments arranged by size: 592 332 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 82 136 145 729 933 954 Size: 82 54 9 584 204 21 Fragments arranged by size: 584 204 82 54 21 9 BseYI C'CCAG_C Cuts at: 0 458 954 Size: 458 496 BsiHKAI G_wGCw'C Cuts at: 0 7 954 Size: 7 947 BsmI GAATG_Cn' Cuts at: 0 144 954 Size: 144 810 BsmBI CGTCTCn'nnnn_ Cuts at: 0 594 954 Size: 594 360 Bsp1286I G_dGCh'C Cuts at: 0 7 173 251 915 954 Size: 7 166 78 664 39 Fragments arranged by size: 664 166 78 39 7 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 742 954 Size: 742 212 BstXI CCAn_nnnn'nTGG Cuts at: 0 274 954 Size: 274 680 BstYI r'GATC_y Cuts at: 0 222 308 954 Size: 222 86 646 Fragments arranged by size: 646 222 86 BtgI C'CryG_G Cuts at: 0 365 954 Size: 365 589 EaeI y'GGCC_r Cuts at: 0 711 728 954 Size: 711 17 226 Fragments arranged by size: 711 226 17 EarI CTCTTCn'nnn_ Cuts at: 0 772 954 Size: 772 182 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 296 359 589 642 954 Size: 296 63 230 53 312 Fragments arranged by size: 312 296 230 63 53 EcoRI G'AATT_C Cuts at: 0 699 954 Size: 699 255 MscI TGG'CCA Cuts at: 0 713 730 954 Size: 713 17 224 Fragments arranged by size: 713 224 17 MslI CAynn'nnrTG Cuts at: 0 187 502 628 790 832 862 954 Size: 187 315 126 162 42 30 92 Fragments arranged by size: 315 187 162 126 92 42 30 MspA1I CmG'CkG Cuts at: 0 414 709 817 954 Size: 414 295 108 137 Fragments arranged by size: 414 295 137 108 NcoI C'CATG_G Cuts at: 0 365 954 Size: 365 589 NspI r_CATG'y Cuts at: 0 85 795 954 Size: 85 710 159 Fragments arranged by size: 710 159 85 PflMI CCAn_nnn'nTGG Cuts at: 0 444 954 Size: 444 510 PvuII CAG'CTG Cuts at: 0 817 954 Size: 817 137 SapI GCTCTTCn'nnn_ Cuts at: 0 772 954 Size: 772 182 SphI G_CATG'C Cuts at: 0 85 954 Size: 85 869 StuI AGG'CCT Cuts at: 0 724 954 Size: 724 230 StyI C'CwwG_G Cuts at: 0 365 725 954 Size: 365 360 229 Fragments arranged by size: 365 360 229 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 392 954 Size: 392 562 TatI w'GTAC_w Cuts at: 0 191 954 Size: 191 763 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 258 954 Size: 258 696 ZraI GAC'GTC Cuts at: 0 588 954 Size: 588 366 Enzymes that do cut: AarI AatII AcuI AleI AlfI AlwNI ApoI AvaI BamHI BanII BbvCI BclI BglI BlpI Bme1580I BmrI BpmI Bpu10I BsaAI BsaHI BsaXI BseRI BseYI BsiHKAI BsmI BsmBI Bsp1286I BspMI BstXI BstYI BtgI EaeI EarI Eco57MI EcoRI MscI MslI MspA1I NcoI NspI PflMI PvuII SapI SphI StuI StyI TaqII TatI XcmI ZraI Enzymes that do not cut: AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AloI ApaI ApaLI AscI AseI AsiSI AvrII BaeI BanI BbeI BbsI BcgI BciVI BfrBI BglII BmgBI BmtI BplI BpuEI BsaI BsaBI BsaWI BsgI BsiEI BsiWI BspEI BspHI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI DraIII DrdI EagI EciI EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI PacI PasI PciI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacI SacII SalI SanDI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SrfI SspI SwaI TstI Tth111I XbaI XhoI XmaI XmnI