(Circular) (Six-base) MAPSORT of: pFN21AE6084.seq Check: 999 from: 1 to: 5677 pFN21AE6084 5677 bp With 182 enzymes: SgfI * December 8, 2014 13:51 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3487 278 Size: 53 83 186 936 1951 2468 Fragments arranged by size: 2468 1951 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3020 1937 Size: 1083 4594 Acc65I G'GTAC_C Cuts at: 2400 2998 2400 Size: 598 5079 AclI AA'CG_TT Cuts at: 1563 3804 4177 1563 Size: 2241 373 3063 Fragments arranged by size: 3063 2241 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2582 3740 5124 5392 1632 Size: 950 1158 1384 268 1917 Fragments arranged by size: 1917 1384 1158 950 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5480 AflIII A'CryG_T Cuts at: 1203 2580 2726 4576 5553 1203 Size: 1377 146 1850 977 1327 Fragments arranged by size: 1850 1377 1327 977 146 AgeI A'CCGG_T Cuts at: 4508 4508 Size: 5677 AhdI GACnn_n'nnGTC Cuts at: 2263 4406 2263 Size: 2143 3534 AleI CACnn'nnGTG Cuts at: 1166 2783 1166 Size: 1617 4060 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5677 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2917 2949 2917 Size: 32 5645 AlwNI CAG_nnn'CTG Cuts at: 3420 4992 3420 Size: 1572 4105 ApaLI G'TGCA_C Cuts at: 3734 4890 3734 Size: 1156 4521 ApoI r'AATT_y Cuts at: 1493 2339 2986 3212 3364 5473 1493 Size: 846 647 226 152 2109 1697 Fragments arranged by size: 2109 1697 846 647 226 152 AseI AT'TA_AT Cuts at: 160 1032 4231 160 Size: 872 3199 1606 Fragments arranged by size: 3199 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5677 AvaI C'yCGr_G Cuts at: 1870 1943 2078 2520 3002 1870 Size: 73 135 442 482 4545 Fragments arranged by size: 4545 482 442 135 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2215 2248 2215 Size: 33 5644 BamHI G'GATC_C Cuts at: 3007 3374 3007 Size: 367 5310 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2400 2998 4453 618 Size: 325 217 627 613 598 1455 1842 Fragments arranged by size: 1842 1455 627 613 598 325 217 BanII G_rGCy'C Cuts at: 1391 2996 1391 Size: 1605 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2012 2092 928 Size: 971 113 80 4513 Fragments arranged by size: 4513 971 113 80 BbvCI CC'TCA_GC Cuts at: 2700 2700 Size: 5677 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3868 3902 1903 Size: 34 1931 34 3678 Fragments arranged by size: 3678 1931 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2209 3569 4779 2209 Size: 1360 1210 3107 Fragments arranged by size: 3107 1360 1210 BclI T'GATC_A Cuts at: 1548 2964 1548 Size: 1416 4261 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4288 136 Size: 107 122 71 631 605 2616 1525 Fragments arranged by size: 2616 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2307 5672 2307 Size: 3365 2312 BlpI GC'TnA_GC Cuts at: 2195 3094 2195 Size: 899 4778 Bme1580I G_kGCm'C Cuts at: 1790 3738 4894 1790 Size: 1948 1156 2573 Fragments arranged by size: 2573 1948 1156 BmgBI CAC'GTC Cuts at: 1318 2583 1318 Size: 1265 4412 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3408 4366 442 Size: 416 53 83 399 24 1991 958 1753 Fragments arranged by size: 1991 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5677 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2009 2237 2793 4337 1239 Size: 770 228 556 1544 2579 Fragments arranged by size: 2579 1544 770 556 228 Bpu10I CC'TnA_GC Cuts at: 2700 2700 Size: 5677 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2902 3802 4667 4965 2902 Size: 900 865 298 3614 Fragments arranged by size: 3614 900 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2360 2588 4340 882 Size: 617 861 228 1752 2219 Fragments arranged by size: 2219 1752 861 617 228 BsaAI yAC'GTr Cuts at: 493 1204 2727 3468 493 Size: 711 1523 741 2702 Fragments arranged by size: 2702 1523 741 711 BsaBI GATnn'nnATC Cuts at: 5671 5671 Size: 5677 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3484 3866 4501 275 Size: 53 83 186 936 1951 382 635 1451 Fragments arranged by size: 1951 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4110 4508 4782 4929 5340 4110 Size: 398 274 147 411 4447 Fragments arranged by size: 4447 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2147 2177 2917 2947 1178 Size: 30 165 30 744 30 740 30 3908 Fragments arranged by size: 3908 744 740 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2399 1186 Size: 1213 4464 BseYI C'CCAG_C Cuts at: 2350 2551 4880 5376 2350 Size: 201 2329 496 2651 Fragments arranged by size: 2651 2329 496 201 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2319 1163 Size: 573 583 4521 Fragments arranged by size: 4521 583 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3127 3888 4916 1540 Size: 275 186 1126 761 1028 2301 Fragments arranged by size: 2301 1126 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3738 3823 4894 3738 Size: 85 1071 4521 Fragments arranged by size: 4521 1071 85 BsmI GAATG_Cn' Cuts at: 3188 3281 3188 Size: 93 5584 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2996 3738 3823 4894 1391 Size: 399 1206 742 85 1071 2174 Fragments arranged by size: 2174 1206 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5340 5340 Size: 5677 BspHI T'CATG_A Cuts at: 2139 2412 3566 5296 2139 Size: 273 1154 1730 2520 Fragments arranged by size: 2520 1730 1154 273 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3032 844 Size: 2188 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3564 1394 Size: 429 1741 3507 Fragments arranged by size: 3507 1741 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4172 4346 65 Size: 1183 2924 174 1396 Fragments arranged by size: 2924 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2706 4321 4508 1700 Size: 110 896 1615 187 2869 Fragments arranged by size: 2869 1615 896 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5677 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5677 BssSI C'ACGA_G Cuts at: 3737 4749 3737 Size: 1012 4665 BstAPI GCAn_nnn'nTGC Cuts at: 794 2427 794 Size: 1633 4044 BstBI TT'CG_AA Cuts at: 4481 4481 Size: 5677 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5677 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2113 2011 Size: 102 5575 BstYI r'GATC_y Cuts at: 1972 2307 2516 2547 3007 3374 3759 3776 5228 Size: 335 209 31 460 367 385 17 1452 Cuts at: 5228 5314 5326 5672 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 460 385 367 346 335 209 86 31 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4186 Fragments arranged by size: 4186 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4938 Fragments arranged by size: 4938 683 56 BtsI GCAGTG_nn' Cuts at: 3193 3987 4007 3193 Size: 794 20 4863 Fragments arranged by size: 4863 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5677 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5642 DraI TTT'AAA Cuts at: 2981 2981 Size: 5677 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4684 809 Size: 507 3368 1802 Fragments arranged by size: 3368 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3124 4013 5642 Size: 54 1425 50 275 18 1294 889 1629 Cuts at: 5642 8 Size: 43 Fragments arranged by size: 1629 1425 1294 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3124 1537 Size: 275 1312 4090 Fragments arranged by size: 4090 1312 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3607 1353 Size: 2254 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4264 4638 4784 387 Size: 1064 138 2675 374 146 1280 Fragments arranged by size: 2675 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2009 2237 2582 2793 3740 4337 5124 Size: 393 377 228 345 211 947 597 787 Cuts at: 5124 5392 1239 Size: 268 1524 Fragments arranged by size: 1524 947 787 597 393 377 345 268 228 211 EcoNI CCTnn'n_nnAGG Cuts at: 2267 2267 Size: 5677 EcoO109I rG'GnC_Cy Cuts at: 2257 2257 Size: 5677 EcoRI G'AATT_C Cuts at: 2339 2986 3364 2339 Size: 647 378 4652 Fragments arranged by size: 4652 647 378 FspI TGC'GCA Cuts at: 4183 4183 Size: 5677 HaeII r_GCGC'y Cuts at: 1678 4824 1678 Size: 3146 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2249 2281 4318 4350 4392 Size: 32 135 32 677 32 2037 32 42 Cuts at: 4392 4424 1373 Size: 32 2626 Fragments arranged by size: 2626 2037 677 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3021 3267 669 Size: 988 281 1083 246 3079 Fragments arranged by size: 3079 1083 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5677 HpaI GTT'AAC Cuts at: 3267 3267 Size: 5677 KpnI G_GTAC'C Cuts at: 2404 3002 2404 Size: 598 5079 MfeI C'AATT_G Cuts at: 3276 3276 Size: 5677 MluI A'CGCG_T Cuts at: 5553 5553 Size: 5677 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4791 4975 1305 Size: 3486 184 2007 Fragments arranged by size: 3486 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3855 Fragments arranged by size: 3855 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2111 2156 2180 2744 2783 2855 Size: 648 843 102 45 24 564 39 72 Cuts at: 2855 2951 3635 3994 4153 518 Size: 96 684 359 159 2042 Fragments arranged by size: 2042 843 684 648 564 359 159 102 96 72 45 39 24 MspA1I CmG'CkG Cuts at: 1606 3093 3770 4918 5163 1606 Size: 1487 677 1148 245 2120 Fragments arranged by size: 2120 1487 1148 677 245 NaeI GCC'GGC Cuts at: 2708 2708 Size: 5677 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4186 Fragments arranged by size: 4186 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5677 NgoMIV G'CCGG_C Cuts at: 2706 2706 Size: 5677 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5677 NotI GC'GGCC_GC Cuts at: 3124 3124 Size: 5677 NspI r_CATG'y Cuts at: 1132 1229 3035 4580 1132 Size: 97 1806 1545 2229 Fragments arranged by size: 2229 1806 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5677 PciI A'CATG_T Cuts at: 4576 4576 Size: 5677 PflMI CCAn_nnn'nTGG Cuts at: 1108 2743 1108 Size: 1635 4042 PfoI T'CCnGG_A Cuts at: 2253 2253 Size: 5677 PmeI GTTT'AAAC Cuts at: 2981 2981 Size: 5677 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2917 2949 3746 3778 5259 5291 2917 Size: 32 797 32 1481 32 3303 Fragments arranged by size: 3303 1481 797 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2257 2257 Size: 5677 PshAI GACnn'nnGTC Cuts at: 1613 2489 1613 Size: 876 4801 PsiI TTA'TAA Cuts at: 3247 3247 Size: 5677 PstI C_TGCA'G Cuts at: 830 2905 3029 830 Size: 2075 124 3478 Fragments arranged by size: 3478 2075 124 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5677 SalI G'TCGA_C Cuts at: 1936 3019 1936 Size: 1083 4594 SbfI CC_TGCA'GG Cuts at: 3029 3029 Size: 5677 ScaI AGT'ACT Cuts at: 2919 3925 5637 2919 Size: 1006 1712 2959 Fragments arranged by size: 2959 1712 1006 SfcI C'TryA_G Cuts at: 826 1045 2512 2875 2901 3025 4160 4841 5032 Size: 219 1467 363 26 124 1135 681 191 Cuts at: 5032 826 Size: 1471 Fragments arranged by size: 1471 1467 1135 681 363 219 191 124 26 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5677 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5677 SmaI CCC'GGG Cuts at: 1872 2080 3004 1872 Size: 208 924 4545 Fragments arranged by size: 4545 924 208 SmlI C'TyrA_G Cuts at: 820 1017 1943 2881 3781 4682 4944 820 Size: 197 926 938 900 901 262 1553 Fragments arranged by size: 1553 938 926 901 900 262 197 SnaBI TAC'GTA Cuts at: 493 3468 493 Size: 2975 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5677 SphI G_CATG'C Cuts at: 1132 3035 1132 Size: 1903 3774 SspI AAT'ATT Cuts at: 5 52 3601 5 Size: 47 3549 2081 Fragments arranged by size: 3549 2081 47 StuI AGG'CCT Cuts at: 1517 2055 1517 Size: 538 5139 StyI C'CwwG_G Cuts at: 513 1065 2004 2268 2466 3116 513 Size: 552 939 264 198 650 3074 Fragments arranged by size: 3074 939 650 552 264 198 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2078 2194 3704 3721 3874 4059 4504 Size: 1255 498 116 1510 17 153 185 445 Cuts at: 4504 325 Size: 1498 Fragments arranged by size: 1510 1498 1255 498 445 185 153 116 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2184 2917 3923 Size: 274 80 33 51 1443 207 733 1006 Cuts at: 3923 5635 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1006 733 274 207 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2147 2179 5637 5669 2147 Size: 32 3458 32 2155 Fragments arranged by size: 3458 2155 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5677 XbaI T'CTAG_A Cuts at: 3013 3013 Size: 5677 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2113 2287 2113 Size: 174 5503 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5677 XmaI C'CCGG_G Cuts at: 1870 2078 3002 1870 Size: 208 924 4545 Fragments arranged by size: 4545 924 208 XmnI GAAnn'nnTTC Cuts at: 3806 3806 Size: 5677 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3485 276 Size: 53 83 186 936 1951 2468 Fragments arranged by size: 2468 1951 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI