################################## # NO CUT ENZYMES ( 143 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AviII AvrII BanI BbeI BbrPI BbvCI BcgI BciVI BfrI BlnI BlpI Bpu1102I BsaBI BsaI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraII DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FseI FspI HaeII HhaI HinP1I HindIII KasI KpnI KspI MamI MluI MroI MspA1I MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI TaqI TthHB8I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AciI 459 CCGC CCGC(-3/-1) AcsI 427 GAATTT [GA]^AATT[CT] AcyI 250 GACGCC G[GA]^CG[CT]C AlwNI 374 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG ApoI 427 GAATTT [GA]^AATT[CT] AspI 346 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 608 CCCGGG C^[CT]CG[GA]G AvaII 345 GGACC G^G[AT]CC BamHI 604 GGATCC G^GATCC BbsI 922 GTCTTC GAAGAC(2/6) BclI 731 TGATCA T^GATCA BglI 442 GCCACCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 368 AGATCT A^GATCT BpmI 941 CTCCAG CTGGAG(16/14) BpuAI 922 GTCTTC GAAGAC(2/6) BsaHI 250 GACGCC G[GA]^CG[CT]C BsoBI 608 CCCGGG C^[CT]CG[GA]G BspHI 500 TCATGA T^CATGA Cac8I 22 GCCAGC GC[ACGT]^[ACGT]GC EcoNI 342 CCTGGACCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT14I 446 CCATGG C^C[AT][AT]GG FbaI 731 TGATCA T^GATCA HgaI 250 GACGC GACGC(5/10) Hin1I 250 GACGCC G[GA]^CG[CT]C HincII 1008 GTTAAC GT[CT]^[GA]AC HindII 1008 GTTAAC GT[CT]^[GA]AC HpaI 1008 GTTAAC GTT^AAC MfeI 289 CAATTG C^AATTG MunI 289 CAATTG C^AATTG NcoI 446 CCATGG C^CATGG NspI 872 ACATGC [GA]CATG^[CT] PflFI 346 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 348 CCAGGTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshAI 831 GACTGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 500 TCATGA T^CATGA SexAI 347 ACCAGGT A^CC[AT]GGT SfcI 963 CTATAG C^T[GA][CT]AG SmaI 608 CCCGGG CCC^GGG StuI 803 AGGCCT AGG^CCT StyI 446 CCATGG C^C[AT][AT]GG Tth111I 346 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 348 CCAGGTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmaCI 608 CCCGGG C^CCGGG XmaI 608 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### BanII 549 GGGCTC G[GA]GC[CT]^C 992 GGGCTC BbvI 536 GCTGC GCAGC(8/12) 856 GCTGC BcnI 608 CCCGG CC^[GC]GG 609 CCGGG BfaI 415 CTAG C^TAG 439 CTAG BsaAI 406 TACGTG [CT]AC^GT[GA] 463 TACGTG BsmAI 294 GTCTC GTCTC(1/5) 518 GTCTC BsmFI 57 GGGAC GGGAC(10/14) 141 GGGAC BstYI 368 AGATCT [GA]^GATC[CT] 604 GGATCC Cfr13I 345 GGACC G^G[ACGT]CC 792 GGGCC Fnu4HI 536 GCTGC GC^[ACGT]GC 856 GCTGC HapII 609 CCGG C^CGG 795 CCGG HpaII 609 CCGG C^CGG 795 CCGG ItaI 536 GCTGC GC^[ACGT]GC 856 GCTGC MaeI 415 CTAG C^TAG 439 CTAG MaeII 407 ACGT A^CGT 464 ACGT MflI 368 AGATCT [GA]^GATC[CT] 604 GGATCC MseI 958 TTAA T^TAA 1009 TTAA MspI 609 CCGG C^CGG 795 CCGG MwoI 330 GCCCAAGATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 442 GCCACCATGGC NciI 608 CCCGG CC^[GC]GG 609 CCGGG PleI 59 GACTC GAGTC(4/5) 143 GACTC Sau96I 345 GGACC G^G[ACGT]CC 792 GGGCC SfaNI 336 GATGC GCATC(5/9) 390 GATGC TfiI 615 GATTC G^A[AT]TC 651 GAATC Tru9I 958 TTAA T^TAA 1009 TTAA TseI 536 GCTGC G^C[AT]GC 856 GCTGC Tsp509I 290 AATT ^AATT 428 AATT XcmI 74 CCAAACATCCAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 332 CCAAGATGCTCCTGG XhoII 368 AGATCT [GA]^GATC[CT] 604 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 42 enzymes) # ######################################### AfaI AluI AlwI BalI BmyI BsaJI BseRI BsiYI BslI Bsp1286I BspMI BsrI BssKI BstF5I BstNI DdeI DpnII DsaI EaeI EarI EcoRII FokI HaeIII HinfI HphI Ksp632I MaeIII MboI MboII MluNI MnlI MscI MslI MvaI NdeII NlaIII NlaIV RsaI Sau3AI ScrFI Tsp45I TspRI