(Circular) (Six-base) MAPSORT of: pFN21AE5495.seq Check: 1429 from: 1 to: 5749 pFN21AE5495 5749 bp With 182 enzymes: SgfI * December 8, 2014 13:46 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2828 2828 Size: 5749 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3559 278 Size: 53 83 186 936 2023 2468 Fragments arranged by size: 2468 2023 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3092 1937 Size: 1155 4594 Acc65I G'GTAC_C Cuts at: 3070 3070 Size: 5749 AclI AA'CG_TT Cuts at: 1563 3876 4249 1563 Size: 2313 373 3063 Fragments arranged by size: 3063 2313 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2053 2137 3023 3812 5196 5464 1632 Size: 421 84 886 789 1384 268 1917 Fragments arranged by size: 1917 1384 886 789 421 268 84 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5552 AflIII A'CryG_T Cuts at: 1203 4648 5625 1203 Size: 3445 977 1327 Fragments arranged by size: 3445 1327 977 AgeI A'CCGG_T Cuts at: 4580 4580 Size: 5749 AhdI GACnn_n'nnGTC Cuts at: 4478 4478 Size: 5749 AleI CACnn'nnGTG Cuts at: 1166 2948 1166 Size: 1782 3967 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5749 AlwNI CAG_nnn'CTG Cuts at: 2384 3492 5064 2384 Size: 1108 1572 3069 Fragments arranged by size: 3069 1572 1108 ApaLI G'TGCA_C Cuts at: 3806 4962 3806 Size: 1156 4593 ApoI r'AATT_y Cuts at: 1493 2432 3058 3284 3436 5545 1493 Size: 939 626 226 152 2109 1697 Fragments arranged by size: 2109 1697 939 626 226 152 AseI AT'TA_AT Cuts at: 160 1032 4303 160 Size: 872 3271 1606 Fragments arranged by size: 3271 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5749 AvaI C'yCGr_G Cuts at: 1870 1943 2613 3074 1870 Size: 73 670 461 4545 Fragments arranged by size: 4545 670 461 73 BamHI G'GATC_C Cuts at: 2609 3079 3446 2609 Size: 470 367 4912 Fragments arranged by size: 4912 470 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3070 4525 618 Size: 325 217 627 1283 1455 1842 Fragments arranged by size: 1842 1455 1283 627 325 217 BanII G_rGCy'C Cuts at: 1391 2558 3001 3068 1391 Size: 1167 443 67 4072 Fragments arranged by size: 4072 1167 443 67 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2920 928 Size: 971 1021 3757 Fragments arranged by size: 3757 1021 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3940 3974 1903 Size: 34 2003 34 3678 Fragments arranged by size: 3678 2003 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3641 4851 3641 Size: 1210 4539 BclI T'GATC_A Cuts at: 1548 2736 1548 Size: 1188 4561 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2453 4360 136 Size: 107 122 71 631 605 781 1907 1525 Fragments arranged by size: 1907 1525 781 631 605 122 107 71 BglII A'GATC_T Cuts at: 2373 5744 2373 Size: 3371 2378 BlpI GC'TnA_GC Cuts at: 3166 3166 Size: 5749 Bme1580I G_kGCm'C Cuts at: 1790 2337 3810 4966 1790 Size: 547 1473 1156 2573 Fragments arranged by size: 2573 1473 1156 547 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5749 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3480 4438 442 Size: 416 53 83 399 24 2063 958 1753 Fragments arranged by size: 2063 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5749 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2931 4409 1239 Size: 1692 1478 2579 Fragments arranged by size: 2579 1692 1478 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3874 4739 5037 3874 Size: 865 298 4586 Fragments arranged by size: 4586 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4412 882 Size: 617 2913 2219 Fragments arranged by size: 2913 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2413 2470 3540 493 Size: 711 1209 57 1070 2702 Fragments arranged by size: 2702 1209 1070 711 57 BsaBI GATnn'nnATC Cuts at: 5743 5743 Size: 5749 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2256 3556 3938 4573 Size: 53 83 186 936 723 1300 382 635 Cuts at: 4573 275 Size: 1451 Fragments arranged by size: 1451 1300 936 723 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4182 4580 4854 5001 5412 4182 Size: 398 274 147 411 4519 Fragments arranged by size: 4519 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2563 2593 2748 2778 1178 Size: 30 165 30 1160 30 155 30 4149 Fragments arranged by size: 4149 1160 165 155 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2711 2756 2815 1186 Size: 1525 45 59 4120 Fragments arranged by size: 4120 1525 59 45 BseYI C'CCAG_C Cuts at: 2885 4952 5448 2885 Size: 2067 496 3186 Fragments arranged by size: 3186 2067 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5176 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3199 3960 4988 1540 Size: 275 186 1198 761 1028 2301 Fragments arranged by size: 2301 1198 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3810 3895 4966 3810 Size: 85 1071 4593 Fragments arranged by size: 4593 1071 85 BsmI GAATG_Cn' Cuts at: 3260 3353 3260 Size: 93 5656 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2337 2558 3001 3068 3810 3895 4966 Size: 399 547 221 443 67 742 85 1071 Cuts at: 4966 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 547 443 399 221 85 67 BspEI T'CCGG_A Cuts at: 5412 5412 Size: 5749 BspHI T'CATG_A Cuts at: 2505 3638 5368 2505 Size: 1133 1730 2886 Fragments arranged by size: 2886 1730 1133 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2414 2828 3034 3104 844 Size: 1570 414 206 70 3489 Fragments arranged by size: 3489 1570 414 206 70 BsrBI CCG'CTC Cuts at: 1394 1823 3636 1394 Size: 429 1813 3507 Fragments arranged by size: 3507 1813 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4244 4418 65 Size: 1183 2996 174 1396 Fragments arranged by size: 2996 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4393 4580 1700 Size: 110 2583 187 2869 Fragments arranged by size: 2869 2583 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5749 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5749 BssSI C'ACGA_G Cuts at: 3809 4821 3809 Size: 1012 4737 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5749 BstBI TT'CG_AA Cuts at: 4553 4553 Size: 5749 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5749 BstYI r'GATC_y Cuts at: 1972 2373 2609 3079 3446 3831 3848 5300 5386 Size: 401 236 470 367 385 17 1452 86 Cuts at: 5386 5398 5744 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 470 401 385 367 346 236 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2451 2605 2776 513 Size: 552 939 447 154 171 3486 Fragments arranged by size: 3486 939 552 447 171 154 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5010 Fragments arranged by size: 5010 683 56 BtsI GCAGTG_nn' Cuts at: 3265 4059 4079 3265 Size: 794 20 4935 Fragments arranged by size: 4935 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5749 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5714 DraI TTT'AAA Cuts at: 3053 3053 Size: 5749 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4756 809 Size: 507 3440 1802 Fragments arranged by size: 3440 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2025 2091 2109 Size: 54 1425 50 275 18 195 66 18 Cuts at: 2109 2472 3196 4085 5714 8 Size: 363 724 889 1629 43 Fragments arranged by size: 1629 1425 889 724 363 275 195 66 54 50 43 18 18 EagI C'GGCC_G Cuts at: 1537 1812 3196 1537 Size: 275 1384 4090 Fragments arranged by size: 4090 1384 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2072 2156 2531 2855 3679 1353 Size: 719 84 375 324 824 3423 Fragments arranged by size: 3423 824 719 375 324 84 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4336 4710 4856 387 Size: 1064 138 2747 374 146 1280 Fragments arranged by size: 2747 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2053 2137 2931 3023 3812 4409 5196 Size: 393 421 84 794 92 789 597 787 Cuts at: 5196 5464 1239 Size: 268 1524 Fragments arranged by size: 1524 794 789 787 597 421 393 268 92 84 EcoNI CCTnn'n_nnAGG Cuts at: 2351 2351 Size: 5749 EcoRI G'AATT_C Cuts at: 3058 3436 3058 Size: 378 5371 FspI TGC'GCA Cuts at: 4255 4255 Size: 5749 HaeII r_GCGC'y Cuts at: 1678 4896 1678 Size: 3218 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4390 4422 4464 4496 1373 Size: 32 135 32 2818 32 42 32 2626 Fragments arranged by size: 2818 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3015 3093 3339 669 Size: 988 281 1077 78 246 3079 Fragments arranged by size: 3079 1077 988 281 246 78 HindIII A'AGCT_T Cuts at: 748 748 Size: 5749 HpaI GTT'AAC Cuts at: 3015 3339 3015 Size: 324 5425 KpnI G_GTAC'C Cuts at: 3074 3074 Size: 5749 MfeI C'AATT_G Cuts at: 2294 3348 2294 Size: 1054 4695 MluI A'CGCG_T Cuts at: 5625 5625 Size: 5749 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2025 2109 4863 5047 1305 Size: 720 84 2754 184 2007 Fragments arranged by size: 2754 2007 720 184 84 MscI TGG'CCA Cuts at: 10 64 1489 1832 2027 2093 2111 2474 10 Size: 54 1425 343 195 66 18 363 3285 Fragments arranged by size: 3285 1425 363 343 195 66 54 18 MslI CAynn'nnrTG Cuts at: 518 1166 2021 2057 2105 2195 2273 2309 2363 Size: 648 855 36 48 90 78 36 54 Cuts at: 2363 2588 2729 2876 2948 3707 4066 4225 518 Size: 225 141 147 72 759 359 159 2042 Fragments arranged by size: 2042 855 759 648 359 225 159 147 141 90 78 72 54 48 36 36 MspA1I CmG'CkG Cuts at: 1606 3165 3842 4990 5235 1606 Size: 1559 677 1148 245 2120 Fragments arranged by size: 2120 1559 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2451 513 Size: 552 939 447 3811 Fragments arranged by size: 3811 939 552 447 NdeI CA'TA_TG Cuts at: 387 387 Size: 5749 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5749 NotI GC'GGCC_GC Cuts at: 3196 3196 Size: 5749 NspI r_CATG'y Cuts at: 1132 1229 2881 3107 4652 1132 Size: 97 1652 226 1545 2229 Fragments arranged by size: 2229 1652 1545 226 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5749 PciI A'CATG_T Cuts at: 4648 4648 Size: 5749 PflMI CCAn_nnn'nTGG Cuts at: 1108 2359 1108 Size: 1251 4498 PfoI T'CCnGG_A Cuts at: 2346 2346 Size: 5749 PmeI GTTT'AAAC Cuts at: 3053 3053 Size: 5749 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3818 3850 5331 5363 3818 Size: 32 1481 32 4204 Fragments arranged by size: 4204 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2840 1613 Size: 1227 4522 PsiI TTA'TAA Cuts at: 3319 3319 Size: 5749 PstI C_TGCA'G Cuts at: 830 3101 830 Size: 2271 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5749 SalI G'TCGA_C Cuts at: 1936 3091 1936 Size: 1155 4594 SbfI CC_TGCA'GG Cuts at: 3101 3101 Size: 5749 ScaI AGT'ACT Cuts at: 3997 5709 3997 Size: 1712 4037 SexAI A'CCwGG_T Cuts at: 2352 2352 Size: 5749 SfcI C'TryA_G Cuts at: 826 1045 2968 3097 4232 4913 5104 826 Size: 219 1923 129 1135 681 191 1471 Fragments arranged by size: 1923 1471 1135 681 219 191 129 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5749 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5749 SmaI CCC'GGG Cuts at: 1872 2615 3076 1872 Size: 743 461 4545 Fragments arranged by size: 4545 743 461 SmlI C'TyrA_G Cuts at: 820 1017 1943 3853 4754 5016 820 Size: 197 926 1910 901 262 1553 Fragments arranged by size: 1910 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3540 493 Size: 3047 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5749 SphI G_CATG'C Cuts at: 1132 3107 1132 Size: 1975 3774 SspI AAT'ATT Cuts at: 5 52 3673 5 Size: 47 3621 2081 Fragments arranged by size: 3621 2081 47 StuI AGG'CCT Cuts at: 1517 2810 1517 Size: 1293 4456 StyI C'CwwG_G Cuts at: 513 1065 2004 2451 3188 513 Size: 552 939 447 737 3074 Fragments arranged by size: 3074 939 737 552 447 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2351 3776 3793 3946 4131 4576 325 Size: 1255 771 1425 17 153 185 445 1498 Fragments arranged by size: 1498 1425 1255 771 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2277 2697 3995 Size: 274 80 33 51 1443 300 420 1298 Cuts at: 3995 5707 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1298 420 300 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2563 2595 5709 5741 2563 Size: 32 3114 32 2571 Fragments arranged by size: 3114 2571 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2354 1316 Size: 1038 4711 XbaI T'CTAG_A Cuts at: 3085 3085 Size: 5749 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2086 2344 2086 Size: 258 5491 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5749 XmaI C'CCGG_G Cuts at: 1870 2613 3074 1870 Size: 743 461 4545 Fragments arranged by size: 4545 743 461 XmnI GAAnn'nnTTC Cuts at: 3878 3878 Size: 5749 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3557 276 Size: 53 83 186 936 2023 2468 Fragments arranged by size: 2468 2023 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI