################################## # NO CUT ENZYMES ( 169 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AspI AviII AvrII BanI BbeI BbrPI BbsI BcgI BciVI BclI BcnI BfaI BfrI BglI BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmBI BspDI BspEI BspLU11I BspMI BsrBI BsrFI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstUI BstZ17I Bsu36I Cac8I CelII CfoI Cfr10I ClaI CpoI DraI DraII DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HpaI HpaII KasI KpnI KspI MaeI MaeII MamI MfeI MluI MroI MseI MspA1I MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfaNI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I StuI StyI SwaI Tru9I Tth111I Van91I XbaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 30 enzymes) # ################################### BalI 95 TGGCCA TGG^CCA BamHI 446 GGATCC G^GATCC BbvCI 186 CCTCAGC CCTCAGC(-5/-2) BglII 908 AGATCT A^GATCT BsaI 509 GGTCTC GGTCTC(1/5) BsgI 222 CTGCAC GTGCAG(16/14) BsmAI 510 GTCTC GTCTC(1/5) BsmFI 198 GTCCC GGGAC(10/14) Bsp1407I 773 TGTACA T^GTACA BsrDI 642 CATTGC GCAATG(2/0) BsrGI 773 TGTACA T^GTACA BstEII 888 GGTGACC G^GT[ACGT]ACC BstPI 888 GGTGACC G^GT[ACGT]ACC BstXI 272 CCATTTCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG EaeI 95 TGGCCA [CT]^GGCC[GA] EarI 79 CTCTTC CTCTTC(1/4) EcoO65I 888 GGTGACC G^GT[ACGT]ACC EcoRI 12 GAATTC G^AATTC HindIII 707 AAGCTT A^AGCTT Ksp632I 79 CTCTTC CTCTTC(1/4) MluNI 95 TGGCCA TGG^CCA MscI 95 TGGCCA TGG^CCA NlaIV 446 GGATCC GG[ACGT]^[ACGT]CC PaeR7I 656 CTCGAG C^TCGAG SfcI 867 CTACAG C^T[GA][CT]AG SmlI 656 CTCGAG C^T[CT][GA]AG SspBI 773 TGTACA T^GTACA SspI 579 AATATT AAT^ATT XcmI 98 CCATCTTTTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 656 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AciI 363 CCGC CCGC(-3/-1) 876 CCGC Asp700I 331 GAATGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 586 GAAAAGATTC AvaI 512 CTCGGG C^[CT]CG[GA]G 656 CTCGAG AvaII 90 GGTCC G^G[AT]CC 207 GGACC BanII 408 GAGCCC G[GA]GC[CT]^C 453 GGGCTC BbvI 263 GCAGC GCAGC(8/12) 760 GCAGC BmyI 408 GAGCCC G[AGT]GC[ACT]^C 453 GGGCTC BpmI 689 CTGGAG CTGGAG(16/14) 929 CTGGAG BsaJI 42 CCTGGG C^C[ACGT][ACGT]GG 450 CCTGGG BsmI 331 GAATGC GAATGC(1/-1) 898 GCATTC BsoBI 512 CTCGGG C^[CT]CG[GA]G 656 CTCGAG Bsp1286I 408 GAGCCC G[AGT]GC[ACT]^C 453 GGGCTC BspHI 404 TCATGA T^CATGA 902 TCATGA Fnu4HI 263 GCAGC GC^[ACGT]GC 760 GCAGC HphI 888 GGTGA GGTGA(8/7) 729 TCACC ItaI 263 GCAGC GC^[ACGT]GC 760 GCAGC RcaI 404 TCATGA T^CATGA 902 TCATGA TaqI 16 TCGA T^CGA 657 TCGA TseI 263 GCAGC G^C[AT]GC 760 GCAGC TthHB8I 16 TCGA T^CGA 657 TCGA XmnI 331 GAATGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 586 GAAAAGATTC ######################################### # 3 OR MORE CUT ENZYMES ( 38 enzymes) # ######################################### AcsI AfaI AluI AlwI ApoI BseRI BsiYI BslI BssKI BstF5I BstNI BstYI Cfr13I DdeI DpnII EcoRII FokI HaeIII HinfI MaeIII MboI MboII MflI MnlI MslI MvaI MwoI NdeII NlaIII RsaI Sau3AI Sau96I ScrFI TfiI Tsp45I Tsp509I TspRI XhoII