Restriction map for
pFN21AE5977
(Circular) (Six-base) MAPSORT of: pFN21AE5977.seq Check: 1311 from: 1 to: 5641
pFN21AE5977 5641 bp
With 182 enzymes: SgfI *
December 8, 2014 13:49 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3451 278
Size: 53 83 186 936 1915 2468
Fragments arranged by size:
2468 1915 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2984 1937
Size: 1047 4594
Acc65I G'GTAC_C
Cuts at: 2962 2962
Size: 5641
AclI AA'CG_TT
Cuts at: 1563 3768 4141 1563
Size: 2205 373 3063
Fragments arranged by size:
3063 2205 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3704 5088 5356 1632
Size: 2072 1384 268 1917
Fragments arranged by size:
2072 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5444
AflIII A'CryG_T
Cuts at: 1203 4540 5517 1203
Size: 3337 977 1327
Fragments arranged by size:
3337 1327 977
AgeI A'CCGG_T
Cuts at: 4472 4472
Size: 5641
AhdI GACnn_n'nnGTC
Cuts at: 4370 4370
Size: 5641
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5641
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5641
AlwNI CAG_nnn'CTG
Cuts at: 3384 4956 3384
Size: 1572 4069
ApaLI G'TGCA_C
Cuts at: 3698 4854 3698
Size: 1156 4485
ApoI r'AATT_y
Cuts at: 1493 2017 2304 2421 2950 3176 3328 5437 1493
Size: 524 287 117 529 226 152 2109 1697
Fragments arranged by size:
2109 1697 529 524 287 226 152 117
AseI AT'TA_AT
Cuts at: 160 1032 4195 160
Size: 872 3163 1606
Fragments arranged by size:
3163 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5641
AvaI C'yCGr_G
Cuts at: 1870 1943 2517 2661 2966 1870
Size: 73 574 144 305 4545
Fragments arranged by size:
4545 574 305 144 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2220 2253 2220
Size: 33 5608
BamHI G'GATC_C
Cuts at: 2451 2971 3338 2451
Size: 520 367 4754
Fragments arranged by size:
4754 520 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2962 4417 618
Size: 325 217 627 1175 1455 1842
Fragments arranged by size:
1842 1455 1175 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2417 2462 2960 1391
Size: 1026 45 498 4072
Fragments arranged by size:
4072 1026 498 45
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4670
BbvCI CC'TCA_GC
Cuts at: 2192 2192
Size: 5641
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3832 3866 1903
Size: 34 1895 34 3678
Fragments arranged by size:
3678 1895 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3533 4743 3533
Size: 1210 4431
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5641
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4252 136
Size: 107 122 71 631 605 2580 1525
Fragments arranged by size:
2580 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2913 5636 2913
Size: 2723 2918
BlpI GC'TnA_GC
Cuts at: 3058 3058
Size: 5641
Bme1580I G_kGCm'C
Cuts at: 1790 3702 4858 1790
Size: 1912 1156 2573
Fragments arranged by size:
2573 1912 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5641
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3372 4330 442
Size: 416 53 83 399 24 1955 958 1753
Fragments arranged by size:
1955 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5641
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2715 2955 4301 1239
Size: 1476 240 1346 2579
Fragments arranged by size:
2579 1476 1346 240
Bpu10I CC'TnA_GC
Cuts at: 2192 2192
Size: 5641
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3766 4631 4929 3766
Size: 865 298 4478
Fragments arranged by size:
4478 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2520 4304 882
Size: 617 1021 1784 2219
Fragments arranged by size:
2219 1784 1021 617
BsaAI yAC'GTr
Cuts at: 493 1204 3432 493
Size: 711 2228 2702
Fragments arranged by size:
2702 2228 711
BsaBI GATnn'nnATC
Cuts at: 5635 5635
Size: 5641
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3448 3830 4465 275
Size: 53 83 186 936 1915 382 635 1451
Fragments arranged by size:
1915 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4074 4472 4746 4893 5304 4074
Size: 398 274 147 411 4411
Fragments arranged by size:
4411 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2534 2564 1178
Size: 30 165 30 1131 30 4255
Fragments arranged by size:
4255 1131 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2135 2177 2180 1186
Size: 949 42 3 4647
Fragments arranged by size:
4647 949 42 3
BseYI C'CCAG_C
Cuts at: 2163 4844 5340 2163
Size: 2681 496 2464
Fragments arranged by size:
2681 2464 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2212 1163
Size: 573 476 4592
Fragments arranged by size:
4592 573 476
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3091 3852 4880 1540
Size: 275 186 1090 761 1028 2301
Fragments arranged by size:
2301 1090 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3702 3787 4858 3702
Size: 85 1071 4485
Fragments arranged by size:
4485 1071 85
BsmI GAATG_Cn'
Cuts at: 2342 2903 3152 3245 2342
Size: 561 249 93 4738
Fragments arranged by size:
4738 561 249 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2417 2462 2960 3702 3787 4858 1391
Size: 399 627 45 498 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 627 498 399 85 45
BspEI T'CCGG_A
Cuts at: 5304 5304
Size: 5641
BspHI T'CATG_A
Cuts at: 2409 2907 3530 5260 2409
Size: 498 623 1730 2790
Fragments arranged by size:
2790 1730 623 498
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2996 844
Size: 2152 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3528 1394
Size: 429 1705 3507
Fragments arranged by size:
3507 1705 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2646 4136 4310 65
Size: 1183 1398 1490 174 1396
Fragments arranged by size:
1490 1398 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4285 4472 1700
Size: 110 2475 187 2869
Fragments arranged by size:
2869 2475 187 110
BsrGI T'GTAC_A
Cuts at: 96 2778 96
Size: 2682 2959
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5641
BssSI C'ACGA_G
Cuts at: 3701 4713 3701
Size: 1012 4629
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5641
BstBI TT'CG_AA
Cuts at: 4445 4445
Size: 5641
BstEII G'GTnAC_C
Cuts at: 1184 2893 1184
Size: 1709 3932
BstXI CCAn_nnnn'nTGG
Cuts at: 2284 2284
Size: 5641
BstYI r'GATC_y
Cuts at: 1972 2451 2690 2913 2971 3338 3723 3740 5192
Size: 479 239 223 58 367 385 17 1452
Cuts at: 5192 5278 5290 5636 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 479 385 367 346 239 223
86 58 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4150
Fragments arranged by size:
4150 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4902
Fragments arranged by size:
4902 683 56
BtsI GCAGTG_nn'
Cuts at: 2321 2373 2578 3157 3951 3971 2321
Size: 52 205 579 794 20 3991
Fragments arranged by size:
3991 794 579 205 52 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5641
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5606
DraI TTT'AAA
Cuts at: 2945 2945
Size: 5641
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4648 809
Size: 507 3332 1802
Fragments arranged by size:
3332 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2100 3088 3977
Size: 54 1425 50 275 18 270 988 889
Cuts at: 3977 5606 8
Size: 1629 43
Fragments arranged by size:
1629 1425 988 889 275 270 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3088 1537
Size: 275 1276 4090
Fragments arranged by size:
4090 1276 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2090 3571 1353
Size: 737 1481 3423
Fragments arranged by size:
3423 1481 737
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4228 4602 4748 387
Size: 1064 138 2639 374 146 1280
Fragments arranged by size:
2639 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2715 2955 3704 4301 5088 5356 1239
Size: 393 1083 240 749 597 787 268 1524
Fragments arranged by size:
1524 1083 787 749 597 393 268 240
EcoRI G'AATT_C
Cuts at: 2017 2950 3328 2017
Size: 933 378 4330
Fragments arranged by size:
4330 933 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2266 2266
Size: 5641
FspI TGC'GCA
Cuts at: 4147 4147
Size: 5641
HaeII r_GCGC'y
Cuts at: 1678 4788 1678
Size: 3110 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2534 2566 4282 4314 4356
Size: 32 135 32 962 32 1716 32 42
Cuts at: 4356 4388 1373
Size: 32 2626
Fragments arranged by size:
2626 1716 962 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2985 3231 669
Size: 988 281 1047 246 3079
Fragments arranged by size:
3079 1047 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2712 748
Size: 1964 3677
HpaI GTT'AAC
Cuts at: 3231 3231
Size: 5641
KpnI G_GTAC'C
Cuts at: 2966 2966
Size: 5641
MfeI C'AATT_G
Cuts at: 3240 3240
Size: 5641
MluI A'CGCG_T
Cuts at: 5517 5517
Size: 5641
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2058 4755 4939 1305
Size: 753 2697 184 2007
Fragments arranged by size:
2697 2007 753 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2102 10
Size: 54 1425 343 270 3549
Fragments arranged by size:
3549 1425 343 270 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2177 2282 2684 2852 3599 3958 4117
Size: 648 1011 105 402 168 747 359 159
Cuts at: 4117 518
Size: 2042
Fragments arranged by size:
2042 1011 747 648 402 359 168 159
105
MspA1I CmG'CkG
Cuts at: 1606 3057 3734 4882 5127 1606
Size: 1451 677 1148 245 2120
Fragments arranged by size:
2120 1451 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4150
Fragments arranged by size:
4150 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5641
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5641
NotI GC'GGCC_GC
Cuts at: 3088 3088
Size: 5641
NspI r_CATG'y
Cuts at: 1132 1229 2999 4544 1132
Size: 97 1770 1545 2229
Fragments arranged by size:
2229 1770 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5641
PciI A'CATG_T
Cuts at: 4540 4540
Size: 5641
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5641
PfoI T'CCnGG_A
Cuts at: 2447 2447
Size: 5641
PmeI GTTT'AAAC
Cuts at: 2945 2945
Size: 5641
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3710 3742 5223 5255 3710
Size: 32 1481 32 4096
Fragments arranged by size:
4096 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5641
PsiI TTA'TAA
Cuts at: 3211 3211
Size: 5641
PspXI vC'TCGA_Gb
Cuts at: 2661 2661
Size: 5641
PstI C_TGCA'G
Cuts at: 830 2993 830
Size: 2163 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5641
SalI G'TCGA_C
Cuts at: 1936 2983 1936
Size: 1047 4594
SbfI CC_TGCA'GG
Cuts at: 2993 2993
Size: 5641
ScaI AGT'ACT
Cuts at: 3889 5601 3889
Size: 1712 3929
SfcI C'TryA_G
Cuts at: 826 1045 2872 2989 4124 4805 4996 826
Size: 219 1827 117 1135 681 191 1471
Fragments arranged by size:
1827 1471 1135 681 219 191 117
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5641
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5641
SmaI CCC'GGG
Cuts at: 1872 2968 1872
Size: 1096 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2661 3745 4646 4908 820
Size: 197 926 718 1084 901 262 1553
Fragments arranged by size:
1553 1084 926 901 718 262 197
SnaBI TAC'GTA
Cuts at: 493 3432 493
Size: 2939 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5641
SphI G_CATG'C
Cuts at: 1132 2999 1132
Size: 1867 3774
SspI AAT'ATT
Cuts at: 5 52 2586 3565 5
Size: 47 2534 979 2081
Fragments arranged by size:
2534 2081 979 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5641
StyI C'CwwG_G
Cuts at: 513 1065 2004 3080 513
Size: 552 939 1076 3074
Fragments arranged by size:
3074 1076 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2087 2177 3668 3685 3838 4023 4468
Size: 1255 507 90 1491 17 153 185 445
Cuts at: 4468 325
Size: 1498
Fragments arranged by size:
1498 1491 1255 507 445 185 153 90
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2754 2778 3887
Size: 274 80 33 51 1443 777 24 1109
Cuts at: 3887 5599 96
Size: 1712 138
Fragments arranged by size:
1712 1443 1109 777 274 138 80 51
33 24
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5601 5633 5601
Size: 32 5609
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5641
XbaI T'CTAG_A
Cuts at: 2977 2977
Size: 5641
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2110 2110
Size: 5641
XhoI C'TCGA_G
Cuts at: 1943 2661 1943
Size: 718 4923
XmaI C'CCGG_G
Cuts at: 1870 2966 1870
Size: 1096 4545
XmnI GAAnn'nnTTC
Cuts at: 2340 2595 3770 2340
Size: 255 1175 4211
Fragments arranged by size:
4211 1175 255
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3449 276
Size: 53 83 186 936 1915 2468
Fragments arranged by size:
2468 1915 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
MslI MspA1I NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PshAI PsiI PspXI
PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BbeI BfrBI
BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI
EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV
NruI NsiI PacI PmlI PpuMI PspOMI PsrI PvuII
RsrII SacI SacII SanDI SapI SexAI SfiI SfoI
SrfI SwaI