################################## # NO CUT ENZYMES ( 172 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcsI AcyI AflIII AgeI AhdI Alw44I AlwNI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AvaII AviII AvrII BalI BamHI BanI BbeI BbrPI BbvCI BcgI BciVI BclI BfaI BglI BglII BlnI BlpI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsiEI BsiWI BsmAI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrFI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I CelII CfoI Cfr10I ClaI CpoI DraI DsaI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI KpnI KspI MaeI MamI MfeI MluI MluNI MroI MscI MspA1I MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AciI 399 CCGC CCGC(-3/-1) AfaI 484 GTAC GT^AC AflII 232 CTTAAG C^TTAAG AluI 191 AGCT AG^CT AocI 870 CCTGAGG CC^T[ACGT]AGG ApaI 893 GGGCCC GGGCC^C AspHI 467 GTGCTC G[AT]GC[AT]^C BanII 893 GGGCCC G[GA]GC[CT]^C BbsI 823 GTCTTC GAAGAC(2/6) BbvI 719 GCAGC GCAGC(8/12) BcnI 654 CCGGG CC^[GC]GG BfrI 232 CTTAAG C^TTAAG BpuAI 823 GTCTTC GAAGAC(2/6) BsgI 323 GTGCAG GTGCAG(16/14) BsiHKAI 467 GTGCTC G[AT]GC[AT]^C BsmFI 538 GTCCC GGGAC(10/14) BspMI 715 ACCTGC ACCTGC(4/8) BstYI 784 AGATCC [GA]^GATC[CT] Bsu36I 870 CCTGAGG CC^T[ACGT]AGG Cac8I 559 GCCTGC GC[ACGT]^[ACGT]GC DraIII 279 CACTGGGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 568 GACACCTGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 651 TGGCCG [CT]^GGCC[GA] Eco81I 870 CCTGAGG CC^T[ACGT]AGG Fnu4HI 719 GCAGC GC^[ACGT]GC HapII 654 CCGG C^CGG HpaII 654 CCGG C^CGG ItaI 719 GCAGC GC^[ACGT]GC MaeII 777 ACGT A^CGT MflI 784 AGATCC [GA]^GATC[CT] MseI 233 TTAA T^TAA MspI 654 CCGG C^CGG MwoI 343 GCATCTATGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NciI 654 CCGGG CC^[GC]GG RsaI 484 GTAC GT^AC SmlI 232 CTTAAG C^T[CT][GA]AG TaqI 454 TCGA T^CGA Tru9I 233 TTAA T^TAA TseI 719 GCAGC G^C[AT]GC Tsp45I 550 GTGAC ^GT[GC]AC TthHB8I 454 TCGA T^CGA XhoII 784 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AccI 670 GTCTAC GT^[AC][GT]AC 763 GTCTAC BsaJI 509 CCAGGG C^C[ACGT][ACGT]GG 572 CCTGGG BsrI 115 ACTGG ACTGG(1/-1) 280 ACTGG BssSI 902 CACGAG CACGAG(-5/-1) 616 CTCGTG BstF5I 81 CATCC GGATG(2/0) 396 CATCC BstNI 509 CCAGG CC^[AT]GG 572 CCTGG DdeI 184 CTTAG C^T[ACGT]AG 871 CTGAG DraII 892 GGGGCCC [GA]G^G[ACGT]CC[CT] 893 GGGCCCT EarI 76 CTCTTC CTCTTC(1/4) 383 CTCTTC EcoO109I 892 GGGGCCC [GA]G^G[ACGT]CC[CT] 893 GGGCCCT EcoRII 509 CCAGG ^CC[AT]GG 572 CCTGG FokI 81 CATCC GGATG(9/13) 396 CATCC HphI 549 GGTGA GGTGA(8/7) 885 GGTGA Ksp632I 76 CTCTTC CTCTTC(1/4) 383 CTCTTC MvaI 509 CCAGG CC^[AT]GG 572 CCTGG NlaIV 892 GGGGCC GG[ACGT]^[ACGT]CC 893 GGGCCC PleI 552 GACTC GAGTC(4/5) 909 GAGTC PstI 686 CTGCAG CTGCA^G 717 CTGCAG SfaNI 241 GCATC GCATC(5/9) 343 GCATC ######################################### # 3 OR MORE CUT ENZYMES ( 25 enzymes) # ######################################### AlwI BmyI BsiYI BslI Bsp1286I BsrDI BssKI Cfr13I DpnII HaeIII HinfI MaeIII MboI MboII MnlI MslI NdeII NlaIII Sau3AI Sau96I ScrFI SfcI TfiI Tsp509I TspRI