################################## # NO CUT ENZYMES ( 183 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspI AvaI AvaII AviII AvrII BamHI BanI BbeI BbrPI BbsI BbvCI BcgI BclI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaJI BsaWI BseAI BseRI BsiEI BsiWI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstNI BstPI BstUI BstXI BstZ17I Bsu36I CelII CfoI Cfr10I ClaI CpoI DraI DraIII DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRII EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HaeII HhaI Hin1I HinP1I HindIII HpaI KasI KpnI Ksp632I KspI MaeIII MamI MfeI MluI MroI MseI MspA1I MunI MvaI MvnI NaeI NarI NcoI NdeI NgoMIV NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfaNI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI Sse8387I SspBI SspI StyI SwaI TaqI Tru9I Tsp45I Tth111I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 33 enzymes) # ################################### AccI 670 GTCTAC GT^[AC][GT]AC AciI 675 CCGC CCGC(-3/-1) AfaI 775 GTAC GT^AC ApaI 893 GGGCCC GGGCC^C Asp700I 915 GAAAATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BalI 368 TGGCCA TGG^CCA BanII 893 GGGCCC G[GA]GC[CT]^C BciVI 491 GTATCC GTATCC(6/5) BcnI 654 CCGGG CC^[GC]GG BfaI 556 CTAG C^TAG BsgI 561 CTGCAC GTGCAG(16/14) BssKI 654 CCGGG ^CC[ACGT]GG BstAPI 754 GCACCCTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC DrdI 568 GACACTTGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC HapII 654 CCGG C^CGG HgaI 552 GACGC GACGC(5/10) HincII 549 GTTGAC GT[CT]^[GA]AC HindII 549 GTTGAC GT[CT]^[GA]AC HpaII 654 CCGG C^CGG MaeI 556 CTAG C^TAG MaeII 777 ACGT A^CGT MluNI 368 TGGCCA TGG^CCA MscI 368 TGGCCA TGG^CCA MspI 654 CCGG C^CGG MwoI 754 GCACCCTTTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NciI 654 CCGGG CC^[GC]GG NheI 555 GCTAGC G^CTAGC PstI 686 CTGCAG CTGCA^G RsaI 775 GTAC GT^AC ScrFI 654 CCGGG CC^[ACGT]GG SphI 677 GCATGC GCATG^C StuI 704 AGGCCT AGG^CCT XmnI 915 GAAAATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AlwI 785 GATCC GGATC(4/5) 436 GGATC AspHI 467 GTGCTC G[AT]GC[AT]^C 585 GAGCAC BbvI 602 GCAGC GCAGC(8/12) 333 GCTGC BsiHKAI 467 GTGCTC G[AT]GC[AT]^C 585 GAGCAC BsiYI 625 CCTTTCACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 647 CCTATGGCCGG BslI 625 CCTTTCACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 647 CCTATGGCCGG BsrI 280 ACTGG ACTGG(1/-1) 631 ACTGG BstYI 436 GGATCT [GA]^GATC[CT] 784 AGATCC Cfr13I 893 GGGCC G^G[ACGT]CC 894 GGCCC DraII 892 GGGGCCC [GA]G^G[ACGT]CC[CT] 893 GGGCCCT EaeI 368 TGGCCA [CT]^GGCC[GA] 651 TGGCCG EcoO109I 892 GGGGCCC [GA]G^G[ACGT]CC[CT] 893 GGGCCCT Fnu4HI 333 GCTGC GC^[ACGT]GC 602 GCAGC ItaI 333 GCTGC GC^[ACGT]GC 602 GCAGC MflI 436 GGATCT [GA]^GATC[CT] 784 AGATCC NlaIV 892 GGGGCC GG[ACGT]^[ACGT]CC 893 GGGCCC NspI 640 GCATGT [GA]CATG^[CT] 677 GCATGC Sau96I 893 GGGCC G^G[ACGT]CC 894 GGCCC TseI 333 GCTGC G^C[AT]GC 602 GCAGC XhoII 436 GGATCT [GA]^GATC[CT] 784 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 22 enzymes) # ######################################### AluI BmyI Bsp1286I BstF5I Cac8I DdeI DpnII FokI HaeIII HinfI HphI MboI MboII MnlI MslI NdeII NlaIII Sau3AI SfcI TfiI Tsp509I TspRI