################################## # NO CUT ENZYMES ( 157 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwNI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BanI BbeI BbsI BbvCI BcgI BciVI BcnI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaHI BsaI BsaWI BseAI BsiEI BsiWI BsmBI BsmFI BsmI BsoBI BspDI BspEI BspLU11I BspMI BsrBI BsrFI BssHII BssSI BstAPI BstBI BstEII BstPI BstUI CelII Cfr10I ClaI CpoI DraII DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO109I EcoO65I EcoRI EcoRV EcoT22I FauI FseI FspI HaeII HgaI Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MaeI MfeI MluI MroI MunI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmeI PpuMI PshBI Psp1406I PstI PvuI RsrII SacI SacII SalI SapI SbfI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspI StuI SwaI TaqI TfiI Tsp509I Tth111I TthHB8I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 35 enzymes) # ################################### AccI 216 GTATAC GT^[AC][GT]AC AocI 675 CCTCAGG CC^T[ACGT]AGG AvaII 758 GGTCC G^G[AT]CC BamHI 680 GGATCC G^GATCC BanII 438 GGGCTC G[GA]GC[CT]^C BbrPI 717 CACGTG CAC^GTG BclI 809 TGATCA T^GATCA BsaAI 717 CACGTG [CT]AC^GT[GA] BsaBI 99 GATGTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseRI 723 CTCCTC GAGGAG(10/8) BspHI 404 TCATGA T^CATGA Bst1107I 216 GTATAC GTA^TAC BstZ17I 216 GTATAC GTA^TAC Bsu36I 675 CCTCAGG CC^T[ACGT]AGG CfoI 424 GCGC GCG^C DraI 852 TTTAAA TTT^AAA EarI 65 CTCTTC CTCTTC(1/4) Eco81I 675 CCTCAGG CC^T[ACGT]AGG FbaI 809 TGATCA T^GATCA HapII 699 CCGG C^CGG HhaI 424 GCGC GCG^C HinP1I 424 GCGC G^CGC HpaII 699 CCGG C^CGG Ksp632I 65 CTCTTC CTCTTC(1/4) MamI 99 GATGTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MseI 853 TTAA T^TAA MspI 699 CCGG C^CGG NcoI 350 CCATGG C^CATGG PmaCI 717 CACGTG CAC^GTG PmlI 717 CACGTG CAC^GTG PshAI 361 GACCGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuII 427 CAGCTG CAG^CTG RcaI 404 TCATGA T^CATGA ScaI 332 AGTACT AGT^ACT Tru9I 853 TTAA T^TAA ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AciI 363 CCGC CCGC(-3/-1) 780 CCGC BalI 245 TGGCCA TGG^CCA 461 TGGCCA BpmI 845 CTCCAG CTGGAG(16/14) 205 CTGGAG BsgI 324 CTGCAC GTGCAG(16/14) 564 CTGCAC BsiYI 671 CCATCCTCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 692 CCAGTGGCCGG BslI 671 CCATCCTCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 692 CCAGTGGCCGG BsmAI 895 GAGAC GTCTC(1/5) 646 GTCTC Bsp1407I 101 TGTACA T^GTACA 656 TGTACA BsrDI 465 CATTGC GCAATG(2/0) 540 CATTGC BsrGI 101 TGTACA T^GTACA 656 TGTACA BstXI 236 CCAAAGCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 248 CCATCCTACTGG BstYI 680 GGATCC [GA]^GATC[CT] 804 GGATCT DsaI 350 CCATGG C^C[GA][CT]GG 737 CCGTGG EcoT14I 350 CCATGG C^C[AT][AT]GG 800 CCTTGG HinfI 843 GACTC G^A[ACGT]TC 897 GACTC MaeIII 536 GTGAC ^GT[ACGT]AC 841 GTGAC MflI 680 GGATCC [GA]^GATC[CT] 804 GGATCT MluNI 245 TGGCCA TGG^CCA 461 TGGCCA MscI 245 TGGCCA TGG^CCA 461 TGGCCA MspA1I 363 CCGCTG C[AC]G^C[GT]G 427 CAGCTG PleI 843 GACTC GAGTC(4/5) 897 GACTC SfaNI 796 GATGC GCATC(5/9) 729 GCATC SspBI 101 TGTACA T^GTACA 656 TGTACA StyI 350 CCATGG C^C[AT][AT]GG 800 CCTTGG Tsp45I 536 GTGAC ^GT[GC]AC 841 GTGAC XhoII 680 GGATCC [GA]^GATC[CT] 804 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 40 enzymes) # ######################################### AfaI AluI AlwI AspHI BbvI BmyI BsaJI BsiHKAI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII EaeI EcoRII Fnu4HI FokI HaeIII HphI ItaI MaeII MboI MboII MnlI MslI MvaI MwoI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI TseI TspRI