(Circular) (Six-base) MAPSORT of: pFN21AE5388.seq Check: 723 from: 1 to: 5629 pFN21AE5388 5629 bp With 182 enzymes: SgfI * January 30, 2014 17:18 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3439 278 Size: 53 83 186 936 1903 2468 Fragments arranged by size: 2468 1903 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2222 2972 1937 Size: 285 750 4594 Fragments arranged by size: 4594 750 285 Acc65I G'GTAC_C Cuts at: 2950 2950 Size: 5629 AclI AA'CG_TT Cuts at: 1563 3756 4129 1563 Size: 2193 373 3063 Fragments arranged by size: 3063 2193 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2057 2930 3692 5076 5344 1632 Size: 425 873 762 1384 268 1917 Fragments arranged by size: 1917 1384 873 762 425 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5432 AflIII A'CryG_T Cuts at: 1203 4528 5505 1203 Size: 3325 977 1327 Fragments arranged by size: 3325 1327 977 AgeI A'CCGG_T Cuts at: 4460 4460 Size: 5629 AhdI GACnn_n'nnGTC Cuts at: 4358 4358 Size: 5629 AleI CACnn'nnGTG Cuts at: 1166 2744 2843 1166 Size: 1578 99 3952 Fragments arranged by size: 3952 1578 99 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5629 AlwNI CAG_nnn'CTG Cuts at: 3372 4944 3372 Size: 1572 4057 ApaLI G'TGCA_C Cuts at: 3686 4842 3686 Size: 1156 4473 ApoI r'AATT_y Cuts at: 1493 2938 3164 3316 5425 1493 Size: 1445 226 152 2109 1697 Fragments arranged by size: 2109 1697 1445 226 152 AseI AT'TA_AT Cuts at: 160 1032 4183 160 Size: 872 3151 1606 Fragments arranged by size: 3151 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5629 AvaI C'yCGr_G Cuts at: 1870 1943 2954 1870 Size: 73 1011 4545 Fragments arranged by size: 4545 1011 73 BamHI G'GATC_C Cuts at: 2685 2959 3326 2685 Size: 274 367 4988 Fragments arranged by size: 4988 367 274 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2950 4405 618 Size: 325 217 627 1163 1455 1842 Fragments arranged by size: 1842 1455 1163 627 325 217 BanII G_rGCy'C Cuts at: 1391 2447 2948 1391 Size: 1056 501 4072 Fragments arranged by size: 4072 1056 501 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4658 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3820 3854 1903 Size: 34 1883 34 3678 Fragments arranged by size: 3678 1883 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3521 4731 3521 Size: 1210 4419 BclI T'GATC_A Cuts at: 1548 2814 1548 Size: 1266 4363 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4240 136 Size: 107 122 71 631 605 2568 1525 Fragments arranged by size: 2568 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5624 5624 Size: 5629 BlpI GC'TnA_GC Cuts at: 3046 3046 Size: 5629 Bme1580I G_kGCm'C Cuts at: 1790 2241 2481 3690 4846 1790 Size: 451 240 1209 1156 2573 Fragments arranged by size: 2573 1209 1156 451 240 BmgBI CAC'GTC Cuts at: 1318 2782 2830 1318 Size: 1464 48 4117 Fragments arranged by size: 4117 1464 48 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2057 2270 3360 Size: 416 53 83 399 24 640 213 1090 Cuts at: 3360 4318 442 Size: 958 1753 Fragments arranged by size: 1753 1090 958 640 416 399 213 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5629 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2231 2835 4289 1239 Size: 992 604 1454 2579 Fragments arranged by size: 2579 1454 992 604 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3754 4619 4917 3754 Size: 865 298 4466 Fragments arranged by size: 4466 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4292 882 Size: 617 2793 2219 Fragments arranged by size: 2793 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2724 3420 493 Size: 711 1520 696 2702 Fragments arranged by size: 2702 1520 711 696 BsaBI GATnn'nnATC Cuts at: 2108 5623 2108 Size: 3515 2114 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3436 3818 4453 275 Size: 53 83 186 936 1903 382 635 1451 Fragments arranged by size: 1903 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4062 4460 4734 4881 5292 4062 Size: 398 274 147 411 4399 Fragments arranged by size: 4399 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2204 2234 1178 Size: 30 165 30 801 30 4573 Fragments arranged by size: 4573 801 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2719 1186 Size: 1533 4096 BseYI C'CCAG_C Cuts at: 4832 5328 4832 Size: 496 5133 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2314 2554 1163 Size: 573 578 240 4238 Fragments arranged by size: 4238 578 573 240 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3079 3840 4868 1540 Size: 275 186 1078 761 1028 2301 Fragments arranged by size: 2301 1078 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2156 2729 2751 3690 3775 4846 2156 Size: 573 22 939 85 1071 2939 Fragments arranged by size: 2939 1071 939 573 85 22 BsmI GAATG_Cn' Cuts at: 3140 3233 3140 Size: 93 5536 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2156 2241 2447 2481 2729 2751 2948 Size: 399 366 85 206 34 248 22 197 Cuts at: 2948 3690 3775 4846 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 366 248 206 197 85 85 34 22 BspEI T'CCGG_A Cuts at: 5292 5292 Size: 5629 BspHI T'CATG_A Cuts at: 2409 3518 5248 2409 Size: 1109 1730 2790 Fragments arranged by size: 2790 1730 1109 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2984 844 Size: 2140 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3516 1394 Size: 429 1693 3507 Fragments arranged by size: 3507 1693 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2469 2544 4124 4298 65 Size: 1183 1221 75 1580 174 1396 Fragments arranged by size: 1580 1396 1221 1183 174 75 BsrFI r'CCGG_y Cuts at: 1700 1810 4273 4460 1700 Size: 110 2463 187 2869 Fragments arranged by size: 2869 2463 187 110 BsrGI T'GTAC_A Cuts at: 96 2106 2661 96 Size: 2010 555 3064 Fragments arranged by size: 3064 2010 555 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5629 BssSI C'ACGA_G Cuts at: 3689 4701 3689 Size: 1012 4617 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5629 BstBI TT'CG_AA Cuts at: 4433 4433 Size: 5629 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5629 BstXI CCAn_nnnn'nTGG Cuts at: 2248 2260 2248 Size: 12 5617 BstYI r'GATC_y Cuts at: 1972 2685 2809 2959 3326 3711 3728 5180 5266 Size: 713 124 150 367 385 17 1452 86 Cuts at: 5266 5278 5624 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 713 385 367 346 150 124 86 17 12 BstZ17I GTA'TAC Cuts at: 2223 2223 Size: 5629 Bsu36I CC'TnA_GG Cuts at: 2681 2681 Size: 5629 BtgI C'CryG_G Cuts at: 513 1065 2004 2355 2742 513 Size: 552 939 351 387 3400 Fragments arranged by size: 3400 939 552 387 351 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4890 Fragments arranged by size: 4890 683 56 BtsI GCAGTG_nn' Cuts at: 2241 2481 3145 3939 3959 2241 Size: 240 664 794 20 3911 Fragments arranged by size: 3911 794 664 240 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5629 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5594 DraI TTT'AAA Cuts at: 2859 2933 2859 Size: 74 5555 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4636 809 Size: 507 3320 1802 Fragments arranged by size: 3320 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2250 2466 2701 Size: 54 1425 50 275 18 420 216 235 Cuts at: 2701 3076 3965 5594 8 Size: 375 889 1629 43 Fragments arranged by size: 1629 1425 889 420 375 275 235 216 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3076 1537 Size: 275 1264 4090 Fragments arranged by size: 4090 1264 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2076 3559 1353 Size: 723 1483 3423 Fragments arranged by size: 3423 1483 723 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4216 4590 4736 387 Size: 1064 138 2627 374 146 1280 Fragments arranged by size: 2627 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2057 2231 2835 2930 3692 4289 5076 Size: 393 425 174 604 95 762 597 787 Cuts at: 5076 5344 1239 Size: 268 1524 Fragments arranged by size: 1524 787 762 604 597 425 393 268 174 95 EcoRI G'AATT_C Cuts at: 2938 3316 2938 Size: 378 5251 FspI TGC'GCA Cuts at: 4135 4135 Size: 5629 HaeII r_GCGC'y Cuts at: 1678 4776 1678 Size: 3098 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4270 4302 4344 4376 1373 Size: 32 135 32 2698 32 42 32 2626 Fragments arranged by size: 2698 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2973 3219 669 Size: 988 281 1035 246 3079 Fragments arranged by size: 3079 1035 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5629 HpaI GTT'AAC Cuts at: 3219 3219 Size: 5629 KpnI G_GTAC'C Cuts at: 2954 2954 Size: 5629 MfeI C'AATT_G Cuts at: 3228 3228 Size: 5629 MluI A'CGCG_T Cuts at: 5505 5505 Size: 5629 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4743 4927 1305 Size: 3438 184 2007 Fragments arranged by size: 3438 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2252 2468 10 Size: 54 1425 343 420 216 3171 Fragments arranged by size: 3171 1425 420 343 216 54 MslI CAynn'nnrTG Cuts at: 518 1166 2474 2744 2843 3587 3946 4105 518 Size: 648 1308 270 99 744 359 159 2042 Fragments arranged by size: 2042 1308 744 648 359 270 159 99 MspA1I CmG'CkG Cuts at: 1606 2370 2434 3045 3722 4870 5115 1606 Size: 764 64 611 677 1148 245 2120 Fragments arranged by size: 2120 1148 764 677 611 245 64 NcoI C'CATG_G Cuts at: 513 1065 2004 2355 513 Size: 552 939 351 3787 Fragments arranged by size: 3787 939 552 351 NdeI CA'TA_TG Cuts at: 387 387 Size: 5629 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5629 NotI GC'GGCC_GC Cuts at: 3076 3076 Size: 5629 NspI r_CATG'y Cuts at: 1132 1229 2987 4532 1132 Size: 97 1758 1545 2229 Fragments arranged by size: 2229 1758 1545 97 PasI CC'CwG_GG Cuts at: 1348 2440 1348 Size: 1092 4537 PciI A'CATG_T Cuts at: 4528 4528 Size: 5629 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5629 PfoI T'CCnGG_A Cuts at: 2208 2208 Size: 5629 PmeI GTTT'AAAC Cuts at: 2933 2933 Size: 5629 PmlI CAC'GTG Cuts at: 2724 2724 Size: 5629 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3698 3730 5211 5243 3698 Size: 32 1481 32 4084 Fragments arranged by size: 4084 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2370 1613 Size: 757 4872 PsiI TTA'TAA Cuts at: 3199 3199 Size: 5629 PstI C_TGCA'G Cuts at: 830 2981 830 Size: 2151 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5629 PvuII CAG'CTG Cuts at: 2434 2434 Size: 5629 SalI G'TCGA_C Cuts at: 1936 2971 1936 Size: 1035 4594 SbfI CC_TGCA'GG Cuts at: 2981 2981 Size: 5629 ScaI AGT'ACT Cuts at: 2339 3877 5589 2339 Size: 1538 1712 2379 Fragments arranged by size: 2379 1712 1538 SfcI C'TryA_G Cuts at: 826 1045 2977 4112 4793 4984 826 Size: 219 1932 1135 681 191 1471 Fragments arranged by size: 1932 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5629 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5629 SmaI CCC'GGG Cuts at: 1872 2956 1872 Size: 1084 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3733 4634 4896 820 Size: 197 926 1790 901 262 1553 Fragments arranged by size: 1790 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3420 493 Size: 2927 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5629 SphI G_CATG'C Cuts at: 1132 2987 1132 Size: 1855 3774 SspI AAT'ATT Cuts at: 5 52 3553 5 Size: 47 3501 2081 Fragments arranged by size: 3501 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5629 StyI C'CwwG_G Cuts at: 513 1065 2004 2355 2805 3068 513 Size: 552 939 351 450 263 3074 Fragments arranged by size: 3074 939 552 450 351 263 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2440 3656 3673 3826 4011 4456 325 Size: 1255 860 1216 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1216 860 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2106 2181 2307 Size: 274 80 33 51 1443 129 75 126 Cuts at: 2307 2337 2661 3875 5587 96 Size: 30 324 1214 1712 138 Fragments arranged by size: 1712 1443 1214 324 274 138 129 126 80 75 51 33 30 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2238 2270 5589 5621 2238 Size: 32 3319 32 2246 Fragments arranged by size: 3319 2246 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5629 XbaI T'CTAG_A Cuts at: 2965 2965 Size: 5629 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5629 XmaI C'CCGG_G Cuts at: 1870 2954 1870 Size: 1084 4545 XmnI GAAnn'nnTTC Cuts at: 3758 3758 Size: 5629 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3437 276 Size: 53 83 186 936 1903 2468 Fragments arranged by size: 2468 1903 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI