Restriction map for
pFN21AE6039
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# NO CUT ENZYMES ( 153 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp718I AspEI AspI AvaI
AviII AvrII BalI BanII BbeI
BbrPI BbsI BbvCI BcgI BclI
BfrI BglI BglII BlnI BpmI
BpuAI BsaAI BsaBI BsaHI BsaWI
BseAI BsgI BsiEI BsiWI BsmBI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrFI BsrGI BsrI
BssHII BssSI Bst1107I BstAPI BstBI
BstUI BstZ17I Bsu36I CfoI Cfr10I
ClaI CpoI DraI DrdI DsaI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoRI EcoRV
FbaI FseI FspI HaeII HhaI
Hin1I HinP1I HincII HindII HpaI
KasI KpnI KspI MamI MfeI
MluI MluNI MroI MscI MunI
MvnI NaeI NarI NcoI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflFI PinAI PleI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SspBI StuI SwaI
TaqI Tsp509I Tth111I TthHB8I XbaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AlwNI 247 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 714 GAAAGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 331 GGATCC G^GATCC
BanI 580 GGCACC G^G[CT][GA]CC
BciVI 617 GTATCC GTATCC(6/5)
BcnI 351 CCCGG CC^[GC]GG
BlpI 598 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 598 GCTGAGC GC^T[ACGT]AGC
BsaI 654 GGTCTC GGTCTC(1/5)
BseRI 657 CTCCTC GAGGAG(10/8)
BsmI 718 GCATTC GAATGC(1/-1)
BsrDI 669 CATTGC GCAATG(2/0)
CelII 598 GCTGAGC GC^T[ACGT]AGC
EarI 835 CTCTTC CTCTTC(1/4)
EcoT14I 437 CCTTGG C^C[AT][AT]GG
EcoT22I 415 ATGCAT ATGCA^T
FauI 854 CCCGC CCCGC(4/6)
HaeIII 435 GGCC GG^CC
HapII 352 CCGG C^CGG
HgaI 344 GCGTC GACGC(5/10)
HindIII 169 AAGCTT A^AGCTT
HinfI 105 GATTC G^A[ACGT]TC
HpaII 352 CCGG C^CGG
Ksp632I 835 CTCTTC CTCTTC(1/4)
MaeII 586 ACGT A^CGT
MspI 352 CCGG C^CGG
NciI 351 CCCGG CC^[GC]GG
NdeI 175 CATATG CA^TATG
NsiI 415 ATGCAT ATGCA^T
NspI 857 GCATGC [GA]CATG^[CT]
PshAI 744 GACAGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 502 CAGCTG CAG^CTG
SmlI 882 CTTGAG C^T[CT][GA]AG
SphI 857 GCATGC GCATG^C
SspI 943 AATATT AAT^ATT
StyI 437 CCTTGG C^C[AT][AT]GG
TfiI 105 GATTC G^A[AT]TC
XcmI 137 CCACCATCATTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 714 GAAAGCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AfaI 216 GTAC GT^AC
481 GTAC
AspHI 94 GTGCTC G[AT]GC[AT]^C
556 GTGCTC
BsiHKAI 94 GTGCTC G[AT]GC[AT]^C
556 GTGCTC
BspMI 727 ACCTGC ACCTGC(4/8)
37 GCAGGT
BstEII 750 GGTCACC G^GT[ACGT]ACC
849 GGTAACC
BstPI 750 GGTCACC G^GT[ACGT]ACC
849 GGTAACC
BstXI 140 CCATCATTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
470 CCACCACCCTGG
BstYI 331 GGATCC [GA]^GATC[CT]
805 AGATCC
DraII 548 AGGTCCC [GA]G^G[ACGT]CC[CT]
724 GGGACCT
DraIII 336 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
676 CACGCAGTG
EcoO109I 548 AGGTCCC [GA]G^G[ACGT]CC[CT]
724 GGGACCT
EcoO65I 750 GGTCACC G^GT[ACGT]ACC
849 GGTAACC
MflI 331 GGATCC [GA]^GATC[CT]
805 AGATCC
MslI 16 CATACCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
774 CATCTTCATG
MspA1I 219 CCGCTG C[AC]G^C[GT]G
502 CAGCTG
PflMI 246 CCAGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
321 CCATGCCCTGG
PpuMI 548 AGGTCCC [GA]G^G[AT]CC[CT]
724 GGGACCT
PstI 499 CTGCAG CTGCA^G
787 CTGCAG
RsaI 216 GTAC GT^AC
481 GTAC
SfaNI 442 GCATC GCATC(5/9)
519 GCATC
SfcI 499 CTGCAG C^T[GA][CT]AG
787 CTGCAG
Tsp45I 368 GTGAC ^GT[GC]AC
751 GTCAC
Van91I 246 CCAGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
321 CCATGCCCTGG
XhoII 331 GGATCC [GA]^GATC[CT]
805 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 42 enzymes) #
#########################################
AciI AluI AlwI AvaII BbvI
BfaI BmyI BsaJI BsiYI BslI
BsmAI BsmFI Bsp1286I BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
EcoRII Fnu4HI FokI HphI ItaI
MaeI MaeIII MboI MboII MnlI
MseI MvaI MwoI NdeII NlaIII
NlaIV Sau3AI Sau96I ScrFI Tru9I
TseI TspRI