(Circular) (Six-base) MAPSORT of: pFN21AE6039.seq Check: 479 from: 1 to: 5656 pFN21AE6039 5656 bp With 182 enzymes: SgfI * August 16, 2021 15:37 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3466 278 Size: 53 83 186 936 1930 2468 Fragments arranged by size: 2468 1930 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2999 1937 Size: 1062 4594 Acc65I G'GTAC_C Cuts at: 2977 2977 Size: 5656 AclI AA'CG_TT Cuts at: 1563 3783 4156 1563 Size: 2220 373 3063 Fragments arranged by size: 3063 2220 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3719 5103 5371 1632 Size: 2087 1384 268 1917 Fragments arranged by size: 2087 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5459 AflIII A'CryG_T Cuts at: 1203 4555 5532 1203 Size: 3352 977 1327 Fragments arranged by size: 3352 1327 977 AgeI A'CCGG_T Cuts at: 4487 4487 Size: 5656 AhdI GACnn_n'nnGTC Cuts at: 4385 4385 Size: 5656 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5656 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5656 AlwNI CAG_nnn'CTG Cuts at: 2257 3399 4971 2257 Size: 1142 1572 2942 Fragments arranged by size: 2942 1572 1142 ApaLI G'TGCA_C Cuts at: 3713 4869 3713 Size: 1156 4500 ApoI r'AATT_y Cuts at: 1493 2965 3191 3343 5452 1493 Size: 1472 226 152 2109 1697 Fragments arranged by size: 2109 1697 1472 226 152 AseI AT'TA_AT Cuts at: 160 1032 4210 160 Size: 872 3178 1606 Fragments arranged by size: 3178 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5656 AvaI C'yCGr_G Cuts at: 1870 1943 2981 1870 Size: 73 1038 4545 Fragments arranged by size: 4545 1038 73 BamHI G'GATC_C Cuts at: 2336 2986 3353 2336 Size: 650 367 4639 Fragments arranged by size: 4639 650 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2585 2977 4432 618 Size: 325 217 627 798 392 1455 1842 Fragments arranged by size: 1842 1455 798 627 392 325 217 BanII G_rGCy'C Cuts at: 1391 2975 1391 Size: 1584 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4685 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3847 3881 1903 Size: 34 1910 34 3678 Fragments arranged by size: 3678 1910 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2633 3548 4758 2633 Size: 915 1210 3531 Fragments arranged by size: 3531 1210 915 BclI T'GATC_A Cuts at: 1548 1548 Size: 5656 BfrBI ATG'CAT Cuts at: 2422 2422 Size: 5656 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4267 136 Size: 107 122 71 631 605 2595 1525 Fragments arranged by size: 2595 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5651 5651 Size: 5656 BlpI GC'TnA_GC Cuts at: 2604 3073 2604 Size: 469 5187 Bme1580I G_kGCm'C Cuts at: 1790 2588 3717 4873 1790 Size: 798 1129 1156 2573 Fragments arranged by size: 2573 1156 1129 798 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5656 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3387 4345 442 Size: 416 53 83 399 24 1970 958 1753 Fragments arranged by size: 1970 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5656 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4316 1239 Size: 3077 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2908 3781 4646 4944 2908 Size: 873 865 298 3620 Fragments arranged by size: 3620 873 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2665 4319 882 Size: 617 1166 1654 2219 Fragments arranged by size: 2219 1654 1166 617 BsaAI yAC'GTr Cuts at: 493 1204 3447 493 Size: 711 2243 2702 Fragments arranged by size: 2702 2243 711 BsaBI GATnn'nnATC Cuts at: 5650 5650 Size: 5656 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3463 3845 4480 275 Size: 53 83 186 936 1930 382 635 1451 Fragments arranged by size: 1930 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4089 4487 4761 4908 5319 4089 Size: 398 274 147 411 4426 Fragments arranged by size: 4426 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5431 Fragments arranged by size: 5431 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2653 1186 Size: 1467 4189 BseYI C'CCAG_C Cuts at: 2250 4859 5355 2250 Size: 2609 496 2551 Fragments arranged by size: 2609 2551 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5083 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3106 3867 4895 1540 Size: 275 186 1105 761 1028 2301 Fragments arranged by size: 2301 1105 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2103 2565 3717 3802 4873 2103 Size: 462 1152 85 1071 2886 Fragments arranged by size: 2886 1152 1071 462 85 BsmI GAATG_Cn' Cuts at: 2723 3167 3260 2723 Size: 444 93 5119 Fragments arranged by size: 5119 444 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2103 2565 2588 2975 3717 3802 4873 Size: 399 313 462 23 387 742 85 1071 Cuts at: 4873 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 462 399 387 313 85 23 BspEI T'CCGG_A Cuts at: 5319 5319 Size: 5656 BspHI T'CATG_A Cuts at: 3545 5275 3545 Size: 1730 3926 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2033 2741 3011 844 Size: 1189 708 270 3489 Fragments arranged by size: 3489 1189 708 270 BsrBI CCG'CTC Cuts at: 1394 1823 3543 1394 Size: 429 1720 3507 Fragments arranged by size: 3507 1720 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2673 4151 4325 65 Size: 1183 1425 1478 174 1396 Fragments arranged by size: 1478 1425 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4300 4487 1700 Size: 110 2490 187 2869 Fragments arranged by size: 2869 2490 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5656 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5656 BssSI C'ACGA_G Cuts at: 3716 4728 3716 Size: 1012 4644 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5656 BstBI TT'CG_AA Cuts at: 4460 4460 Size: 5656 BstEII G'GTnAC_C Cuts at: 1184 2755 2854 1184 Size: 1571 99 3986 Fragments arranged by size: 3986 1571 99 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2152 2482 2011 Size: 141 330 5185 Fragments arranged by size: 5185 330 141 BstYI r'GATC_y Cuts at: 1972 2336 2810 2986 3353 3738 3755 5207 5293 Size: 364 474 176 367 385 17 1452 86 Cuts at: 5293 5305 5651 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 474 385 367 364 346 176 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4165 Fragments arranged by size: 4165 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2510 487 Size: 683 56 1284 3633 Fragments arranged by size: 3633 1284 683 56 BtsI GCAGTG_nn' Cuts at: 2245 2691 3172 3966 3986 2245 Size: 446 481 794 20 3915 Fragments arranged by size: 3915 794 481 446 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5656 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5621 DraI TTT'AAA Cuts at: 2960 2960 Size: 5656 DraIII CAC_nnn'GTG Cuts at: 2346 2686 2346 Size: 340 5316 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4663 809 Size: 507 3347 1802 Fragments arranged by size: 3347 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3103 3992 5621 Size: 54 1425 50 275 18 1273 889 1629 Cuts at: 5621 8 Size: 43 Fragments arranged by size: 1629 1425 1273 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3103 1537 Size: 275 1291 4090 Fragments arranged by size: 4090 1291 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2846 3586 1353 Size: 1493 740 3423 Fragments arranged by size: 3423 1493 740 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4243 4617 4763 387 Size: 1064 138 2654 374 146 1280 Fragments arranged by size: 2654 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3719 4316 5103 5371 1239 Size: 393 2087 597 787 268 1524 Fragments arranged by size: 2087 1524 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2554 2730 2554 Size: 176 5480 EcoRI G'AATT_C Cuts at: 2965 3343 2965 Size: 378 5278 FspI TGC'GCA Cuts at: 4162 4162 Size: 5656 HaeII r_GCGC'y Cuts at: 1678 4803 1678 Size: 3125 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4297 4329 4371 4403 1373 Size: 32 135 32 2725 32 42 32 2626 Fragments arranged by size: 2725 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3000 3246 669 Size: 988 281 1062 246 3079 Fragments arranged by size: 3079 1062 988 281 246 HindIII A'AGCT_T Cuts at: 748 2174 748 Size: 1426 4230 HpaI GTT'AAC Cuts at: 3246 3246 Size: 5656 KpnI G_GTAC'C Cuts at: 2981 2981 Size: 5656 MfeI C'AATT_G Cuts at: 3255 3255 Size: 5656 MluI A'CGCG_T Cuts at: 5532 5532 Size: 5656 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4770 4954 1305 Size: 3465 184 2007 Fragments arranged by size: 3465 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3834 Fragments arranged by size: 3834 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2025 2783 3614 3973 4132 518 Size: 648 843 16 758 831 359 159 2042 Fragments arranged by size: 2042 843 831 758 648 359 159 16 MspA1I CmG'CkG Cuts at: 1606 2226 2509 3072 3749 4897 5142 1606 Size: 620 283 563 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 620 563 283 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4165 Fragments arranged by size: 4165 939 552 NdeI CA'TA_TG Cuts at: 387 2181 387 Size: 1794 3862 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5656 NotI GC'GGCC_GC Cuts at: 3103 3103 Size: 5656 NsiI A_TGCA'T Cuts at: 2424 2424 Size: 5656 NspI r_CATG'y Cuts at: 1132 1229 2866 3014 4559 1132 Size: 97 1637 148 1545 2229 Fragments arranged by size: 2229 1637 1545 148 97 PasI CC'CwG_GG Cuts at: 1348 2332 1348 Size: 984 4672 PciI A'CATG_T Cuts at: 4555 4555 Size: 5656 PflMI CCAn_nnn'nTGG Cuts at: 1108 2257 2332 1108 Size: 1149 75 4432 Fragments arranged by size: 4432 1149 75 PmeI GTTT'AAAC Cuts at: 2960 2960 Size: 5656 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3725 3757 5238 5270 3725 Size: 32 1481 32 4111 Fragments arranged by size: 4111 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2554 2730 2554 Size: 176 5480 PshAI GACnn'nnGTC Cuts at: 1613 2753 1613 Size: 1140 4516 PsiI TTA'TAA Cuts at: 3226 3226 Size: 5656 PstI C_TGCA'G Cuts at: 830 2508 2796 3008 830 Size: 1678 288 212 3478 Fragments arranged by size: 3478 1678 288 212 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5656 PvuII CAG'CTG Cuts at: 2509 2509 Size: 5656 SalI G'TCGA_C Cuts at: 1936 2998 1936 Size: 1062 4594 SbfI CC_TGCA'GG Cuts at: 3008 3008 Size: 5656 ScaI AGT'ACT Cuts at: 3904 5616 3904 Size: 1712 3944 SfcI C'TryA_G Cuts at: 826 1045 2504 2792 3004 4139 4820 5011 826 Size: 219 1459 288 212 1135 681 191 1471 Fragments arranged by size: 1471 1459 1135 681 288 219 212 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5656 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5656 SmaI CCC'GGG Cuts at: 1872 2983 1872 Size: 1111 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2887 3760 4661 4923 820 Size: 197 926 944 873 901 262 1553 Fragments arranged by size: 1553 944 926 901 873 262 197 SnaBI TAC'GTA Cuts at: 493 3447 493 Size: 2954 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5656 SphI G_CATG'C Cuts at: 1132 2866 3014 1132 Size: 1734 148 3774 Fragments arranged by size: 3774 1734 148 SspI AAT'ATT Cuts at: 5 52 2950 3580 5 Size: 47 2898 630 2081 Fragments arranged by size: 2898 2081 630 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5656 StyI C'CwwG_G Cuts at: 513 1065 2004 2442 3095 513 Size: 552 939 438 653 3074 Fragments arranged by size: 3074 939 653 552 438 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3683 3700 3853 4038 4483 325 Size: 1255 2103 17 153 185 445 1498 Fragments arranged by size: 2103 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3902 5614 96 Size: 274 80 33 51 1443 1925 1712 138 Fragments arranged by size: 1925 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5616 5648 5616 Size: 32 5624 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5656 XbaI T'CTAG_A Cuts at: 2992 2992 Size: 5656 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2149 2149 Size: 5656 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5656 XmaI C'CCGG_G Cuts at: 1870 2981 1870 Size: 1111 4545 XmnI GAAnn'nnTTC Cuts at: 2723 3785 2723 Size: 1062 4594 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3464 276 Size: 53 83 186 936 1930 2468 Fragments arranged by size: 2468 1930 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI