Restriction map for
pFN21AE6039
(Linear) (Six-base) MAPSORT of: KIEE6039.seq Check: 7328 from: 1 to: 951
KIEE6039.orf
With 182 enzymes: SgfI *
August 16, 2021 14:16 ..
AlwNI CAG_nnn'CTG
Cuts at: 0 252 951
Size: 252 699
BamHI G'GATC_C
Cuts at: 0 331 951
Size: 331 620
BanI G'GyrC_C
Cuts at: 0 580 951
Size: 580 371
BciVI GTATCCnnnnn_n'
Cuts at: 0 628 951
Size: 628 323
BfrBI ATG'CAT
Cuts at: 0 417 951
Size: 417 534
BlpI GC'TnA_GC
Cuts at: 0 599 951
Size: 599 352
Bme1580I G_kGCm'C
Cuts at: 0 583 951
Size: 583 368
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 903 951
Size: 903 48
BsaI GGTCTCn'nnnn_
Cuts at: 0 660 951
Size: 660 291
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 648 951
Size: 648 303
BseYI C'CCAG_C
Cuts at: 0 245 951
Size: 245 706
BsiHKAI G_wGCw'C
Cuts at: 0 98 560 951
Size: 98 462 391
Fragments arranged by size:
462 391 98
BsmI GAATG_Cn'
Cuts at: 0 718 951
Size: 718 233
Bsp1286I G_dGCh'C
Cuts at: 0 98 560 583 951
Size: 98 462 23 368
Fragments arranged by size:
462 368 98 23
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 28 736 951
Size: 28 708 215
Fragments arranged by size:
708 215 28
BsrDI GCAATG_nn'
Cuts at: 0 668 951
Size: 668 283
BstEII G'GTnAC_C
Cuts at: 0 750 849 951
Size: 750 99 102
Fragments arranged by size:
750 102 99
BstXI CCAn_nnnn'nTGG
Cuts at: 0 147 477 951
Size: 147 330 474
Fragments arranged by size:
474 330 147
BstYI r'GATC_y
Cuts at: 0 331 805 951
Size: 331 474 146
Fragments arranged by size:
474 331 146
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 505 951
Size: 505 446
BtsI GCAGTG_nn'
Cuts at: 0 240 686 951
Size: 240 446 265
Fragments arranged by size:
446 265 240
DraIII CAC_nnn'GTG
Cuts at: 0 341 681 951
Size: 341 340 270
Fragments arranged by size:
341 340 270
EarI CTCTTCn'nnn_
Cuts at: 0 841 951
Size: 841 110
EcoO109I rG'GnC_Cy
Cuts at: 0 549 725 951
Size: 549 176 226
Fragments arranged by size:
549 226 176
HindIII A'AGCT_T
Cuts at: 0 169 951
Size: 169 782
MslI CAynn'nnrTG
Cuts at: 0 20 778 951
Size: 20 758 173
Fragments arranged by size:
758 173 20
MspA1I CmG'CkG
Cuts at: 0 221 504 951
Size: 221 283 447
Fragments arranged by size:
447 283 221
NdeI CA'TA_TG
Cuts at: 0 176 951
Size: 176 775
NsiI A_TGCA'T
Cuts at: 0 419 951
Size: 419 532
NspI r_CATG'y
Cuts at: 0 861 951
Size: 861 90
PasI CC'CwG_GG
Cuts at: 0 327 951
Size: 327 624
PflMI CCAn_nnn'nTGG
Cuts at: 0 252 327 951
Size: 252 75 624
Fragments arranged by size:
624 252 75
PpuMI rG'GwC_Cy
Cuts at: 0 549 725 951
Size: 549 176 226
Fragments arranged by size:
549 226 176
PshAI GACnn'nnGTC
Cuts at: 0 748 951
Size: 748 203
PstI C_TGCA'G
Cuts at: 0 503 791 951
Size: 503 288 160
Fragments arranged by size:
503 288 160
PvuII CAG'CTG
Cuts at: 0 504 951
Size: 504 447
SfcI C'TryA_G
Cuts at: 0 499 787 951
Size: 499 288 164
Fragments arranged by size:
499 288 164
SmlI C'TyrA_G
Cuts at: 0 882 951
Size: 882 69
SphI G_CATG'C
Cuts at: 0 861 951
Size: 861 90
SspI AAT'ATT
Cuts at: 0 945 951
Size: 945 6
StyI C'CwwG_G
Cuts at: 0 437 951
Size: 437 514
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 144 951
Size: 144 807
XmnI GAAnn'nnTTC
Cuts at: 0 718 951
Size: 718 233
Enzymes that do cut:
AlwNI BamHI BanI BciVI BfrBI BlpI Bme1580I BpuEI
BsaI BseRI BseYI BsiHKAI BsmI Bsp1286I BspMI BsrDI
BstEII BstXI BstYI BtgZI BtsI DraIII EarI EcoO109I
HindIII MslI MspA1I NdeI NsiI NspI PasI PflMI
PpuMI PshAI PstI PvuII SfcI SmlI SphI SspI
StyI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI ApaI ApaLI
ApoI AscI AseI AsiSI AvaI AvrII BaeI BanII
BbeI BbsI BbvCI BcgI BclI BglI BglII BmgBI
BmrI BmtI BplI BpmI Bpu10I BsaAI BsaBI BsaHI
BsaWI BsaXI BsgI BsiEI BsiWI BsmBI BspEI BspHI
BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstZ17I
Bsu36I BtgI ClaI CspCI DraI DrdI EaeI EagI
EciI EcoICRI Eco57MI EcoNI EcoRI EcoRV FalI FseI
FspI FspAI HaeII Hin4I HincII HpaI KasI KpnI
MfeI MluI MmeI MscI NaeI NarI NcoI NgoMIV
NheI NotI NruI PacI PciI PfoI PmeI PmlI
PpiI PsiI PspOMI PspXI PsrI PvuI RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI StuI
SwaI TaqII TatI TstI Tth111I XbaI XhoI XmaI
ZraI