################################## # NO CUT ENZYMES ( 139 enzymes) # ################################## AatII Acc65I AccII AccIII AcyI AflII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BamHI BanI BbeI BbrPI BbvCI BciVI BclI BcnI BfrI BglI BlnI BlpI Bpu1102I BsaAI BsaHI BsaI BsaWI BseAI BseRI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspMI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstPI BstUI BstXI Bsu36I CelII CfoI Cfr10I ClaI CpoI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRV FauI FbaI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HpaI HpaII KasI KpnI KspI MluI MroI MspI MvnI NaeI NarI NciI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI PvuI RcaI RsrII SacI SacII SapI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI StuI SwaI Tth111I Van91I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AciI 4 GCGG CCGC(-3/-1) AclI 334 AACGTT AA^CGTT AflIII 1531 ACATGT A^C[GA][CT]GT AlwI 237 GATCC GGATC(4/5) AlwNI 229 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG AspHI 350 GTGCTC G[AT]GC[AT]^C AvaI 1677 CTCGGG C^[CT]CG[GA]G AvaII 231 GGACC G^G[AT]CC BanII 1649 GGGCTC G[GA]GC[CT]^C BcgI 453 CGAAATGCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BpmI 1439 CTCCAG CTGGAG(16/14) BsiHKAI 350 GTGCTC G[AT]GC[AT]^C BsmAI 1097 GTCTC GTCTC(1/5) BsmFI 43 GGGAC GGGAC(10/14) BsoBI 1677 CTCGGG C^[CT]CG[GA]G BspLU11I 1531 ACATGT A^CATGT BsrDI 1030 GCAATG GCAATG(2/0) Bst1107I 1596 GTATAC GTA^TAC BstZ17I 1596 GTATAC GTA^TAC DraI 775 TTTAAA TTT^AAA DraII 230 AGGACCT [GA]G^G[ACGT]CC[CT] DsaI 869 CCATGG C^C[GA][CT]GG EcoNI 1320 CCTTTCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 230 AGGACCT [GA]G^G[ACGT]CC[CT] EcoRI 1813 GAATTC G^AATTC EcoT22I 1500 ATGCAT ATGCA^T HincII 1231 GTCGAC GT[CT]^[GA]AC HindII 1231 GTCGAC GT[CT]^[GA]AC MaeII 335 ACGT A^CGT MfeI 1025 CAATTG C^AATTG MslI 928 CATCCTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 71 CAGCTG C[AC]G^C[GT]G MunI 1025 CAATTG C^AATTG MwoI 1347 GCAGTATAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NcoI 869 CCATGG C^CATGG NsiI 1500 ATGCAT ATGCA^T PleI 1640 GACTC GAGTC(4/5) PpuMI 230 AGGACCT [GA]G^G[AT]CC[CT] Psp1406I 334 AACGTT AA^CGTT PstI 355 CTGCAG CTGCA^G PvuII 71 CAGCTG CAG^CTG SalI 1231 GTCGAC G^TCGAC SfaNI 885 GATGC GCATC(5/9) SmlI 719 CTCAAG C^T[CT][GA]AG SphI 1761 GCATGC GCATG^C TaqI 1232 TCGA T^CGA TthHB8I 1232 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AccI 1231 GTCGAC GT^[AC][GT]AC 1596 GTATAC BalI 1140 TGGCCA TGG^CCA 1904 TGGCCA BglII 1358 AGATCT A^GATCT 1796 AGATCT BmyI 350 GTGCTC G[AGT]GC[ACT]^C 1649 GGGCTC BsaBI 161 GATTGCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1405 GATTTAGATC Bsp1286I 350 GTGCTC G[AGT]GC[ACT]^C 1649 GGGCTC BstYI 1358 AGATCT [GA]^GATC[CT] 1796 AGATCT Cfr13I 231 GGACC G^G[ACGT]CC 1735 GGGCC EaeI 1140 TGGCCA [CT]^GGCC[GA] 1904 TGGCCA EarI 263 CTCTTC CTCTTC(1/4) 340 GAAGAG EcoT14I 869 CCATGG C^C[AT][AT]GG 1143 CCAAGG HindIII 299 AAGCTT A^AGCTT 944 AAGCTT Ksp632I 263 CTCTTC CTCTTC(1/4) 340 GAAGAG MamI 161 GATTGCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1405 GATTTAGATC MflI 1358 AGATCT [GA]^GATC[CT] 1796 AGATCT MluNI 1140 TGGCCA TGG^CCA 1904 TGGCCA MscI 1140 TGGCCA TGG^CCA 1904 TGGCCA NlaIV 1878 GGCTCC GG[ACGT]^[ACGT]CC 1885 GGAACC Sau96I 231 GGACC G^G[ACGT]CC 1735 GGGCC ScaI 214 AGTACT AGT^ACT 1371 AGTACT SspI 688 AATATT AAT^ATT 1295 AATATT StyI 869 CCATGG C^C[AT][AT]GG 1143 CCAAGG Tsp45I 784 GTCAC ^GT[GC]AC 1471 GTCAC XcmI 314 CCAGTCAGGATATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1636 CCAAGACTCTTGTGG XhoII 1358 AGATCT [GA]^GATC[CT] 1796 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 47 enzymes) # ######################################### AcsI AfaI AluI ApoI Asp700I BbsI BbvI BfaI BpuAI BsaJI BsgI BsiYI BslI BsrI BssKI BstF5I BstNI Cac8I DdeI DpnII EcoRII Fnu4HI FokI HaeIII HinfI HphI ItaI MaeI MaeIII MboI MboII MnlI MseI MvaI NdeII NlaIII NspI RsaI Sau3AI ScrFI SfcI TfiI Tru9I TseI Tsp509I TspRI XmnI