################################## # NO CUT ENZYMES ( 112 enzymes) # ################################## AatII Acc65I AccIII AcsI AflII AflIII AgeI AhdI Alw44I Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BamHI BbrPI BciVI BfrI BlnI BlpI Bpu1102I BsaAI BsaBI BsaI BsaWI BseAI BsiEI BsiWI BsmAI BsmBI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrGI BssHII BssSI BstAPI CelII ClaI CpoI DraII DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoO109I EcoRI EcoRV EcoT22I FseI FspI HindIII HpaI KpnI MamI MfeI MluI MroI MunI NcoI NdeI NheI NotI NruI NsiI NspI PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI PstI PvuI RsrII SacI SalI SapI SbfI ScaI SfcI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tth111I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AccI 907 GTATAC GT^[AC][GT]AC AclI 413 AACGTT AA^CGTT AlwI 953 GATCC GGATC(4/5) AocI 306 CCTGAGG CC^T[ACGT]AGG Asp700I 706 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaI 6 CTCGGG C^[CT]CG[GA]G BbeI 72 GGCGCC GGCGC^C BbsI 928 GTCTTC GAAGAC(2/6) BcgI 362 GCAGCCTCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BcnI 112 CCCGG CC^[GC]GG BfaI 556 CTAG C^TAG BglI 460 GCCTGGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 708 AGATCT A^GATCT BpmI 462 CTGGAG CTGGAG(16/14) BpuAI 928 GTCTTC GAAGAC(2/6) BseRI 232 CTCCTC GAGGAG(10/8) BsgI 561 CTGCAC GTGCAG(16/14) BsmI 689 GAATGC GAATGC(1/-1) BsoBI 6 CTCGGG C^[CT]CG[GA]G BspHI 932 TCATGA T^CATGA Bst1107I 907 GTATAC GTA^TAC BstXI 657 CCATTGCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstZ17I 907 GTATAC GTA^TAC Bsu36I 306 CCTGAGG CC^T[ACGT]AGG DraI 738 TTTAAA TTT^AAA Eco81I 306 CCTGAGG CC^T[ACGT]AGG EcoNI 302 CCTGCCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT14I 581 CCAAGG C^C[AT][AT]GG HgaI 164 GCGTC GACGC(5/10) KasI 72 GGCGCC G^GCGCC KspI 53 CCGCGG CCGC^GG MaeI 556 CTAG C^TAG NarI 72 GGCGCC GG^CGCC NciI 112 CCCGG CC^[GC]GG PflMI 1015 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Psp1406I 413 AACGTT AA^CGTT PvuII 1019 CAGCTG CAG^CTG RcaI 932 TCATGA T^CATGA SacII 53 CCGCGG CCGC^GG SexAI 851 ACCTGGT A^CC[AT]GGT SfiI 459 GGCCTGGAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 72 GGCGCC GGC^GCC SgrAI 74 CGCCGGCG C[GA]^CCGG[CT]G StuI 997 AGGCCT AGG^CCT StyI 581 CCAAGG C^C[AT][AT]GG Van91I 1015 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 149 CCATTGGGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 706 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 34 enzymes) # ################################### AcyI 72 GGCGCC G[GA]^CG[CT]C 163 GGCGTC AluI 965 AGCT AG^CT 1020 AGCT AlwNI 964 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG 1016 CAGCAGCTG AspHI 184 GTGCTC G[AT]GC[AT]^C 509 GAGCAC AvaII 429 GGTCC G^G[AT]CC 1172 GGTCC BalI 666 TGGCCA TGG^CCA 758 TGGCCA BanII 90 GAGCCC G[GA]GC[CT]^C 144 GGGCTC BsaHI 72 GGCGCC G[GA]^CG[CT]C 163 GGCGTC BsiHKAI 184 GTGCTC G[AT]GC[AT]^C 509 GAGCAC BspMI 176 ACCTGC ACCTGC(4/8) 260 ACCTGC BsrDI 384 CATTGC GCAATG(2/0) 658 CATTGC BsrFI 69 GCCGGC [GA]^CCGG[CT] 75 GCCGGC BstBI 686 TTCGAA TT^CGAA 947 TTCGAA BstEII 282 GGTCACC G^GT[ACGT]ACC 847 GGTCACC BstPI 282 GGTCACC G^GT[ACGT]ACC 847 GGTCACC BstYI 708 AGATCT [GA]^GATC[CT] 952 AGATCC Cfr10I 69 GCCGGC [GA]^CCGG[CT] 75 GCCGGC EcoO65I 282 GGTCACC G^GT[ACGT]ACC 847 GGTCACC Hin1I 72 GGCGCC G[GA]^CG[CT]C 163 GGCGTC HincII 241 GTCAAC GT[CT]^[GA]AC 1147 GTTGAC HindII 241 GTCAAC GT[CT]^[GA]AC 1147 GTTGAC HinfI 595 GATTC G^A[ACGT]TC 1135 GAATC MaeII 414 ACGT A^CGT 545 ACGT MflI 708 AGATCT [GA]^GATC[CT] 952 AGATCC MluNI 666 TGGCCA TGG^CCA 758 TGGCCA MscI 666 TGGCCA TGG^CCA 758 TGGCCA NaeI 69 GCCGGC GCC^GGC 75 GCCGGC NgoMIV 69 GCCGGC G^CCGGC 75 GCCGGC NspV 686 TTCGAA TT^CGAA 947 TTCGAA SfaNI 586 GATGC GCATC(5/9) 804 GATGC SfuI 686 TTCGAA TT^CGAA 947 TTCGAA TfiI 595 GATTC G^A[AT]TC 1135 GAATC Tsp509I 448 AATT ^AATT 720 AATT XhoII 708 AGATCT [GA]^GATC[CT] 952 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 64 enzymes) # ######################################### AccII AciI AfaI BanI BbvCI BbvI BclI BmyI BsaJI BsiYI BslI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII DsaI EaeI EarI EcoRII FauI FbaI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HpaII HphI ItaI Ksp632I MaeIII MboI MboII MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI TaqI Tru9I TseI Tsp45I TspRI TthHB8I